INHIBIN FROM CULTURES OF RAT SEMINIFEROUS TUBULES

1978 ◽  
Vol 78 (2) ◽  
pp. 217-224 ◽  
Author(s):  
L. W. EDDIE ◽  
H. W. G. BAKER ◽  
A. DULMANIS ◽  
R. E. HIGGINSON ◽  
B. HUDSON
Keyword(s):  
Luteinizing Hormone ◽  
Active Material ◽  
Gel Filtration ◽  
Seminiferous Tubules ◽  
Pituitary Cells ◽  
Basal Secretion ◽  
Luteinizing Hormone Releasing Hormone ◽  
Rat Pituitary ◽  
Rat Pituitary Cells ◽  
Lh Rh

SUMMARY Medium from cultures of mature rat seminiferous tubules contained a substance which suppressed, in a dose-related manner, the luteinizing hormone releasing hormone (LH-RH)-stimulated secretion of FSH by cultured rat pituitary cells. The secretion of LH was suppressed to a lesser extent and the basal secretion of both LH and FSH was inconsistently affected. Gel filtration on Sephadex G-100 did not fractionate the activity. The active material did not inhibit the secretion of TSH or destroy LH-RH and the activity was not due to testosterone or oestradiol in the medium. Control media from liver cultures were inactive. It is concluded that inhibin is present in media from cultures of rat seminiferous tubules.

Melatonin inhibits luteinizing hormone releasing hormone (LHRH) induction of LH release from fetal rat pituitary cells

1995 ◽  
Vol 184 (2) ◽  
pp. 109-112 ◽  
Author(s):  
Atsuhiko Hattori ◽  
Damon C. Herbert ◽  
Mary K. Vaughan ◽  
Ken Yaga ◽  
Russel J. Reiter
Keyword(s):  
Luteinizing Hormone ◽  
Pituitary Cells ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Fetal Rat ◽  
Rat Pituitary ◽  
Lh Release ◽  
Rat Pituitary Cells

RESPONSE OF LUTEINIZING HORMONE FROM COLUMNS OF DISPERSED RAT PITUITARY CELLS TO A HIGHLY POTENT ANALOGUE OF LUTEINIZING HORMONE RELEASING HORMONE

1981 ◽  
Vol 91 (1) ◽  
pp. 33-41 ◽  
Author(s):  
T. YEO ◽  
A. GROSSMAN ◽  
P. BELCHETZ ◽  
G. M. BESSER
Keyword(s):  
Luteinizing Hormone ◽  
Single Pulse ◽  
Pituitary Cells ◽  
Response Curves ◽  
Releasing Hormone ◽  
Rat Pituitary ◽  
Constant Dose ◽  
Rat Pituitary Cells ◽  
Lh Rh ◽  
Lh Releasing Hormone

The response of LH from a perfused column of dispersed rat anterior pituitary cells to LH releasing hormone (LH-RH) and the analogue, d-Ser(But)6-desGly10-Proethylamide9-LH-RH (Hoe 766), was investigated. Dose–response curves showed non-parallelism between LH-RH and the analogue, but it was evident that the analogue was considerably more potent. After a single pulse of LH-RH, LH output returned to basal values in 8 min; this was prolonged to 20 min in the case of the analogue. During this 20 min the cells were refractory to pulses of LH-RH but pulses of the analogue maintained output of LH. During constant-dose perfusion with either synthetic LH-RH or the analogue, output of LH rapidly reached a peak and then gradually fell over several hours to approach baseline values. However, a pulse of 50 mmol potassium chloride/l was still able to release LH at this time. The data are consistent with the view that this analogue of LH-RH is highly potent and is strongly bound by the LH-RH receptor. Furthermore, since it desensitizes the LH-RH receptor, it appears that continued turnover of either LH-RH or the analogue at the receptor is necessary for output of LH to be maintained.

Sensitivity of rat adenohypophyseal cells to estradiol and LHRH during long-term culture

1981 ◽  
Vol 240 (6) ◽  
pp. E602-E608
Author(s):  
L. Lagace ◽  
F. Labrie ◽  
T. Antakly ◽  
G. Pelletier
Keyword(s):  
Luteinizing Hormone ◽  
Cell Types ◽  
Thyroid Stimulating Hormone ◽  
Time Interval ◽  
Pituitary Cells ◽  
Luteinizing Hormone Releasing Hormone ◽  
Lh Release ◽  
Lh Rh ◽  
Stimulating Hormone

To determine possible effects of the time in culture on the responsiveness of the different pituitary cell types to estrogens, rat anterior pituitary cells were incubated up to 20 days in the presence or absence of 10 nM 17 beta-estradiol. Whereas spontaneous luteinizing hormone (LH) and thyroid-stimulating hormone (TSH) release decreased by 85-90%, follicle-stimulating hormone (FSH) and prolactin accumulation in medium were only 50% decreased after 20 days in culture, thus suggesting that the secretion of FSH and prolactin is less dependent on extrinsic stimulatory factors. Estradiol increased spontaneous LH release and its responsiveness to luteinizing hormone-releasing hormone (LH-RH) up to day 16 in culture, whereas the stimulatory effect of the estrogen on FSH secretion was significant only up to day 6. The stimulatory effect of estradiol on basal TSH release was seen up to day 8 in culture, whereas that on spontaneous prolactin release increased progressively after day 8 in culture up to the last time interval studied (20 days). As revealed by immunocytochemistry, the stimulatory effect of estradiol was not due to changes of cell growth.

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