Short-term timecourse of bilateral pudendal nerve injury on leak-point pressure in female rats

Although serotonergic agents have been used to treat patients with stress urinary incontinence, the characteristics of the external urethral sphincter (EUS) activity activated by 5-HT receptors have not been extensively studied. This study examined the effects of the 5-HT1A receptor agonist, 8-hydroxy-2-(di- n-propylamino)tetralin (8-OH-DPAT), on the EUS-electromyography and resistance of the urethra in a rat model with bilateral pudendal nerve injury (BPNI). Two measurements were utilized to assess the effects of the drug on bladder and urethral functions: the simultaneous recordings of transvesical pressure under isovolumetric conditions [isovolumetric intravesical pressure (IVP)] and urethral perfusion pressure, and the simultaneous recordings of IVP during continuously isotonic transvesical infusion with an open urethra (isotonic IVP) and EUS-electromyography. This study also evaluated the urethral continence using leak point pressure testing. The urethral perfusion pressure and leak point pressure measurements of BPNI rats reveal that 8-OH-DPAT significantly increased urethral resistance during the bladder storage phase, yet decreased resistance during the voiding phase. The entire EUS burst period was significantly prolonged, within which the average silent period increased and the frequency of burst discharges decreased. 8-OH-DPAT also improved the voiding efficiency, as evidenced by the detection of decreases in the contraction amplitude and residual volume, with increases in contraction duration and voided volume. These findings suggest that 8-OH-DPAT not only improved continence function, but also elevated the voiding function in a BPNI rat model.

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Urethral compensatory mechanisms to maintain urinary continence after pudendal nerve injury in female rats

International Urogynecology Journal ◽

10.1007/s00192-011-1403-6 ◽

2011 ◽

Vol 22 (8) ◽

pp. 963-970 ◽

Cited By ~ 6

Author(s):

Akira Furuta ◽

Yasuyuki Suzuki ◽

Koji Asano ◽

William C. de Groat ◽

Shin Egawa ◽

...

Keyword(s):

Nerve Injury ◽

Pudendal Nerve ◽

Female Rats ◽

Urinary Continence ◽

Compensatory Mechanisms ◽

Pudendal Nerve Injury

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Pudendal nerve injury reduces urethral outlet resistance in diabetic rats

AJP Renal Physiology ◽

10.1152/ajprenal.00341.2009 ◽

2010 ◽

Vol 299 (6) ◽

pp. F1443-F1450 ◽

Cited By ~ 10

Author(s):

Hui Q. Pan ◽

Dan L. Lin ◽

Christopher Strauch ◽

Robert S. Butler ◽

Vincent M. Monnier ◽

...

Keyword(s):

Nerve Injury ◽

Pudendal Nerve ◽

Outlet Obstruction ◽

Diabetic Rats ◽

Sprague Dawley ◽

External Urethral Sphincter ◽

Nerve Crush ◽

Point Pressure ◽

Functional Outlet Obstruction ◽

Pudendal Nerve Injury

Diabetics have voiding and continence dysfunction to which elevated levels of advanced glycation end products (AGE) may contribute. In addition, pudendal nerve injury is correlated with voiding dysfunction and stress incontinence in rats. The aim of this study was to investigate whether pudendal nerve crush (PNC) in diabetic rats alters urinary function. Female virgin Sprague-Dawley rats (144) were divided equally into diabetic, diuretic, and control groups. Half of the animals in each group were subjected to PNC, and the other half to sham PNC. Diabetes was induced 8 wk before PNC or sham PNC by streptozotocin injection (35 mg/kg). Animals underwent conscious cystometry and leak point pressure (LPP) testing 4 or 13 days after PNC or sham PNC. Tissues of half the animals were tested for levels of AGEs. Qualitative histological assessment was performed in the remaining animals. Diabetic rats 4 days after PNC voided significantly greater volume in a shorter time and with significantly less pressure than after sham PNC, suggesting that diabetic rats have a functional outlet obstruction that is relieved by PNC. LPP was significantly reduced 4 days after PNC in diabetic and diuretic animals and returned to normal 13 days after PNC. Diabetic rats with PNC demonstrated increased muscle fiber disruption and atrophy of the external urethral sphincter. AGEs were significantly elevated in diabetic rats. PNC relieves a functional outlet obstruction in diabetic rats. AGEs are elevated in diabetic rats and could play a role in urinary dysfunction and recovery from PNC.

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Diagnostic and interventional magnetic resonance neurography diagnosis of brachytherapy seed-mediated pudendal nerve injury: a case report

Translational Andrology and Urology ◽

10.21037/tau.2020.03.22 ◽

2020 ◽

Vol 9 (3) ◽

pp. 1442-1447

Author(s):

Luke W. Bonham ◽

Amin S. Herati ◽

Edward F. McCarthy ◽

A. Lee Dellon ◽

Jan Fritz

Keyword(s):

Case Report ◽

Magnetic Resonance ◽

Nerve Injury ◽

Pudendal Nerve ◽

Magnetic Resonance Neurography ◽

Seed Mediated ◽

Pudendal Nerve Injury

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Pudendal Nerve Injury After a Sciatic Nerve Block by the Posterior Approach

Anesthesia & Analgesia ◽

10.1213/ane.0b013e3181e36994 ◽

2010 ◽

Vol 111 (2) ◽

pp. 573-575 ◽

Cited By ~ 5

Author(s):

Attila Bondar ◽

Michael Egan ◽

Denis Jochum ◽

Gérard Amarenco ◽

Hervé Bouaziz

Keyword(s):

Sciatic Nerve ◽

Nerve Injury ◽

Nerve Block ◽

Pudendal Nerve ◽

Posterior Approach ◽

Sciatic Nerve Block ◽

Pudendal Nerve Injury

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Rat Mesenchymal Stem Cell Secretome Promotes Elastogenesis and Facilitates Recovery from Simulated Childbirth Injury

Cell Transplantation ◽

10.3727/096368913x670921 ◽

2014 ◽

Vol 23 (11) ◽

pp. 1395-1406 ◽

Cited By ~ 41

Author(s):

Charuspong Dissaranan ◽

Michelle A. Cruz ◽

Matthew J. Kiedrowski ◽

Brian M. Balog ◽

Bradley C. Gill ◽

...

Keyword(s):

Female Rats ◽

Urethral Sphincter ◽

Leak Point Pressure ◽

External Urethral Sphincter ◽

Paracrine Factors ◽

Pelvic Organs ◽

Point Pressure ◽

Urethral Smooth Muscle ◽

And Function ◽

Vaginal Distension

Vaginal delivery is a risk factor for stress urinary incontinence (SUI). Mesenchymal stem cells (MSCs) home to injured organs and can facilitate repair. The goal of this study was to determine if MSCs home to pelvic organs after simulated childbirth injury and facilitate recovery from SUI via paracrine factors. Three experiments were performed. Eighteen female rats received vaginal distension (VD) or sham VD and labeled intravenous (IV) MSCs to investigate if MSCs home to the pelvic organs. Whole-organ imaging and immunofluorescence were performed 1 week later. Thirty-four female rats received VD and IV MSCs, VD and IV saline, or sham VD and IV saline to investigate if MSCs accelerate recovery of continence. Twenty-nine female rats received VD and periurethral concentrated conditioned media (CCM), VD and periurethral control media, or sham VD and periurethral control media to investigate if factors secreted by MSCs accelerate recovery from VD. Urethral histology and function were assessed 1 week later. Significantly more MSCs were observed in the urethra, vagina, and spleen after VD compared to sham VD. Continence as measured by leak point pressure (LPP) was significantly reduced after VD in rats treated with saline or control media compared to sham VD but not in those given MSCs or CCM. External urethral sphincter (EUS) function as measured by electromyography (EMG) was not improved with MSCs or CCM. Rats treated with MSCs or CCM demonstrated an increase in elastin fibers near the EUS and urethral smooth muscle more similar to that of sham-injured animals than rats treated with saline or control media. MSCs homed to the urethra and vagina and facilitated recovery of continence most likely via secretion of paracrine factors. Both MSCs and CCM have promise as novel noninvasive therapies for SUI.

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Diabetes slows the recovery from urinary incontinence due to simulated childbirth in female rats

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.00686.2006 ◽

2007 ◽

Vol 293 (2) ◽

pp. R950-R955 ◽

Cited By ~ 10

Author(s):

Ja-Hong Kim ◽

Xiao Huang ◽

Guiming Liu ◽

Courtenay Moore ◽

James Bena ◽

...

Keyword(s):

Urinary Incontinence ◽

External Sphincter ◽

Bladder Capacity ◽

Diabetic Rats ◽

Female Rats ◽

Sprague Dawley ◽

Leak Point Pressure ◽

Sprague Dawley Rats ◽

Point Pressure ◽

Vaginal Distension

This study was done to test the hypothesis that simulated vaginal birth by vaginal distension (VD) causes more severe urinary incontinence and slower recovery in diabetic rats. After measuring baseline leak point pressure (LPP) in 16 diabetes mellitus (DM) and 16 age- and weight-matched control (Ct) female Sprague-Dawley rats, these animals underwent either VD or sham VD (sham). Four and ten days after the procedures, LPP and conscious cystometry were assessed. Tissues were then harvested and examined by light microscopy. LPP at baseline was equal among all four groups. Four days after VD, LPP in both VD groups dropped to significantly lower levels than in sham rats ( P < 0.001). Moreover, LPP in the DM+VD group was significantly lower than in the Ct+VD group. At 10 days, LPP in the Ct+VD group had recovered to its baseline value, whereas the LPP in the DM+VD group remained significantly reduced. DM rats had larger bladder capacity and longer voiding intervals than Ct rats. Histological findings included more severe damage to the external sphincter striated musculature of the urethra in DM+VD group compared with Ct+VD. In conclusion, these findings suggest that DM causes increased severity and delayed functional recovery from the effects of simulated childbirth.

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