Accreditation of a multiplex real time PCR assay for detection and semi-quantitative determination of pathogens responsible of sexually-transmitted infections

2018 ◽  
Vol 76 (4) ◽  
pp. 459-476
Author(s):  
Sylvain Robinet ◽  
François Parisot
Keyword(s):  
Quantitative Determination ◽  
Sexually Transmitted Infections ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Sexually Transmitted

Usefulness of a novel multiplex real-time PCR assay for the diagnosis of sexually-transmitted infections

2016 ◽  
Vol 34 (8) ◽  
pp. 471-476 ◽  
Author(s):  
Gema Fernández ◽  
Elisa Martró ◽  
Victoria González ◽  
Verónica Saludes ◽  
Elisabet Bascuñana ◽  
...  
Keyword(s):  
Sexually Transmitted Infections ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Sexually Transmitted

scholarly journals A one-step method for quantitative determination of uracil in DNA by real-time PCR

2010 ◽  
Vol 38 (21) ◽  
pp. e196-e196 ◽  
Author(s):  
András Horváth ◽  
Beáta G. Vértessy
Keyword(s):  
Quantitative Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
Step Method ◽  
One Step

Real-time PCR-assay in the delivery suite for determination of group B streptococcal colonization in a setting with risk-based antibiotic prophylaxis

2013 ◽  
Vol 27 (4) ◽  
pp. 328-332 ◽  
Author(s):  
Stellan Håkansson ◽  
Karin Källén ◽  
Maria Bullarbo ◽  
Per-Åke Holmgren ◽  
Katarina Bremme ◽  
...  
Keyword(s):  
Antibiotic Prophylaxis ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Group B

scholarly journals Applicability of a duplex and four singleplex real-time PCR assays for the qualitative and quantitative determination of wild boar and domestic pig meat in processed food products

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Maria Kaltenbrunner ◽  
Walter Mayer ◽  
Kirsten Kerkhoff ◽  
Rita Epp ◽  
Hermann Rüggeberg ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Wild Boar ◽  
Real Time ◽  
Real Time Pcr ◽  
Processed Food ◽  
Domestic Pig ◽  
The Real ◽  
Qualitative And Quantitative ◽  
Pcr Assays

Abstract Appropriate analytical methods are needed for the detection of food authentication. We investigated the applicability of a duplex real-time PCR assay targeting chromosome 1 and two singleplex real-time PCR assays targeting chromosome 9, both published recently, for the qualitative and quantitative determination of wild boar and domestic pig in processed food products. In addition, two singleplex real-time PCR assays targeting chromosome 7 were tested for their suitability to differentiate the two subspecies. Even by targeting the three genome loci, the probability of misclassification was not completely eliminated. Application of the real-time PCR assays to a total of 35 commercial meat products, including 22 goulash products, revealed that domestic pig DNA was frequently present, even in 14 out of 15 products declared to consist of 100% wild boar. Quantitative results obtained with the real-time PCR assays for wild boar (p < 0.001) and those for domestic pig (p < 0.001) were significantly different. However, the results obtained with the real-time PCR assays for wild boar (r = 0.673; p < 0.001) and those for domestic pig (r = 0.505; p = 0.002) were found to be significantly correlated. If the rules given in the paper are followed, the real-time PCR assays are applicable for routine analysis.

scholarly journals A comparison of oligonucleotide-based microarray and real-time PCR for the detection of sexually transmitted infections

2013 ◽  
Vol 7 (1) ◽  
pp. 68-74 ◽  
Author(s):  
Gyeong-In Lee ◽  
Jong Pil Yoen ◽  
Jin Seok Kang ◽  
Seung Yong Hwang ◽  
Yu-Min Hong ◽  
...  
Keyword(s):  
Sexually Transmitted Infections ◽  
Real Time ◽  
Real Time Pcr ◽  
Sexually Transmitted

Evaluation of VIASURE Sexually Transmitted Diseases Real Time PCR Detection kit (CerTest Biotec) for the diagnostic of sexually transmitted infections

2021 ◽  
Vol 39 (5) ◽  
pp. 229-233
Author(s):  
Cristina Casañ López ◽  
Belén Rivaya Sánchez ◽  
Gema Fernández Rivas ◽  
Águeda Hernández Rodríguez ◽  
Adrián Antuori Torres ◽  
...  
Keyword(s):  
Sexually Transmitted Infections ◽  
Sexually Transmitted Diseases ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Detection ◽  
Sexually Transmitted

Evaluation of VIASURE Sexually Transmitted Diseases Real Time PCR Detection kit (CerTest Biotec) for the diagnostic of sexually transmitted infections

2020 ◽  
Author(s):  
Cristina Casañ López ◽  
Belén Rivaya Sánchez ◽  
Gema Fernández Rivas ◽  
Águeda Hernández Rodríguez ◽  
Adrián Antouri Torres ◽  
...  
Keyword(s):  
Sexually Transmitted Infections ◽  
Sexually Transmitted Diseases ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Detection ◽  
Sexually Transmitted
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