Development an immuno real-time PCR assay for the quantitative determination of Clostridium botulinum toxin by using poultry immunoglobulins (IgY)

2016 ◽  
Author(s):  
Kenan Midilli
Keyword(s):  
Botulinum Toxin ◽  
Quantitative Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
Clostridium Botulinum ◽  
Pcr Assay

scholarly journals A one-step method for quantitative determination of uracil in DNA by real-time PCR

2010 ◽  
Vol 38 (21) ◽  
pp. e196-e196 ◽  
Author(s):  
András Horváth ◽  
Beáta G. Vértessy
Keyword(s):  
Quantitative Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
Step Method ◽  
One Step

Real-time PCR-assay in the delivery suite for determination of group B streptococcal colonization in a setting with risk-based antibiotic prophylaxis

2013 ◽  
Vol 27 (4) ◽  
pp. 328-332 ◽  
Author(s):  
Stellan Håkansson ◽  
Karin Källén ◽  
Maria Bullarbo ◽  
Per-Åke Holmgren ◽  
Katarina Bremme ◽  
...  
Keyword(s):  
Antibiotic Prophylaxis ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Group B

scholarly journals Applicability of a duplex and four singleplex real-time PCR assays for the qualitative and quantitative determination of wild boar and domestic pig meat in processed food products

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Maria Kaltenbrunner ◽  
Walter Mayer ◽  
Kirsten Kerkhoff ◽  
Rita Epp ◽  
Hermann Rüggeberg ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Wild Boar ◽  
Real Time ◽  
Real Time Pcr ◽  
Processed Food ◽  
Domestic Pig ◽  
The Real ◽  
Qualitative And Quantitative ◽  
Pcr Assays

Abstract Appropriate analytical methods are needed for the detection of food authentication. We investigated the applicability of a duplex real-time PCR assay targeting chromosome 1 and two singleplex real-time PCR assays targeting chromosome 9, both published recently, for the qualitative and quantitative determination of wild boar and domestic pig in processed food products. In addition, two singleplex real-time PCR assays targeting chromosome 7 were tested for their suitability to differentiate the two subspecies. Even by targeting the three genome loci, the probability of misclassification was not completely eliminated. Application of the real-time PCR assays to a total of 35 commercial meat products, including 22 goulash products, revealed that domestic pig DNA was frequently present, even in 14 out of 15 products declared to consist of 100% wild boar. Quantitative results obtained with the real-time PCR assays for wild boar (p < 0.001) and those for domestic pig (p < 0.001) were significantly different. However, the results obtained with the real-time PCR assays for wild boar (r = 0.673; p < 0.001) and those for domestic pig (r = 0.505; p = 0.002) were found to be significantly correlated. If the rules given in the paper are followed, the real-time PCR assays are applicable for routine analysis.

scholarly journals Determination of Sex Ratio In Bovine Semen Using SYBR Green Real-Time PCR

2022 ◽  
Author(s):  
Vemula Harshini ◽  
S.M.K. Karthickeyan ◽  
K.G P. Kumarasamy ◽  
Tirumurugaan ◽  
C. Jeevan
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sybr Green ◽  
Pcr Assay ◽  
Mean Values ◽  
Bovine Semen ◽  
Routine Basis ◽  
Repeatability And Reproducibility ◽  
The Mean

Abstract A SYBR green real-time PCR assay was developed to find out the sex skewness in bovine sex-sorted semen samples. The qPCR assay of PLP and SRY genes revealed the mean values of X- and Y-bearing spermatozoa as 50.24 ± 0.65 and 49.75 ± 0.62 per cent in unsorted, and 91.80 ± 0.79 and 8.20 ± 0.73 per cent in X-enriched semen samples respectively.. The amplification efficiencies of the PLP and SRY primers were 99.25 and 98.03 per cent respectively. The method was validated by a series of repeatability and reproducibility assays which revealed low co-efficients of variations as 2.19 and 3.12 per cent respectively Thus becoming a reliable and inexpensive tool to evaluate the sorted semen on routine basis and validation of other sperm sexing technologies.

The use of real time PCR for quantitative determination of some propionic bacteria residing on human skin

2012 ◽  
Vol 58 (5) ◽  
pp. 608-612
Author(s):  
A.G. Globa ◽  
Y.I. Alekseev ◽  
V.G. Arzumanyan ◽  
V.A. Zaborova ◽  
A.A. Guridov
Keyword(s):  
Quantitative Determination ◽  
Real Time ◽  
Human Skin ◽  
Real Time Pcr ◽  
Bacterial Species ◽  
Test System ◽  
Control Group ◽  
The Real

A test system has been developed for determination of propionic bacterial species residing on human skin. This system developed in the real time PCR format is applicable for quantitative determination and also detection of genomes of the following Propionibacterium species: P. acnes, P. granulosum and P. avidum. This system was used for analysis of wash samples from the skin of 17 pentathlon sportsmen and 16 students. All three species of propionic bacteria were found in all skin wash samples. However, contamination with P. acnes was two times higher in control group than in the group of pentathlon sportsmen.

scholarly journals A Highly Sensitive Quantitative Real-Time PCR Assay for Determination of Mutant JAK2 Exon 12 Allele Burden

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33100 ◽  
Author(s):  
Lasse Kjær ◽  
Maj Westman ◽  
Caroline Hasselbalch Riley ◽  
Estrid Høgdall ◽  
Ole Weis Bjerrum ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay ◽  
Allele Burden ◽  
Highly Sensitive
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