Multiplex PCR assay to detect rust resistance genes Lr26 and Lr37 in wheat

Multiplex PCR was developed and optimized for simultaneous detection of wheat leaf rust resistance genes Lr26 and Lr37. The presence of the genes was analyzed in 21 winter wheat cultivars registered in the Czech Republic. Gene Lr37 was detected in four tested cultivars (Bakfis, Biscay, Nicol, Mulan), gene Lr26 occurred only in one cultivar (Etela) and three cultivars (Clarus, Orlando and Rapsodia) were found to carry both these genes. Data obtained by PCR markers were compared with results of greenhouse and field tests. Seedling reactions of cultivars possessing Lr26 to seven different leaf rust isolates conformed to the results obtained by the marker analysis, however, there were found some discrepancies in the detections of Lr37, which could be detected in greenhouse seedling tests only with difficulties.

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Identification of Leaf Rust Resistance Genes in Selected Wheat Cultivars and Development of Multiplex PCR

Open Life Sciences ◽

10.1515/biol-2019-0036 ◽

2019 ◽

Vol 14 (1) ◽

pp. 327-334 ◽

Cited By ~ 2

Author(s):

Agnieszka Tomkowiak ◽

Roksana Skowrońska ◽

Alicja Buda ◽

Danuta Kurasiak-Popowska ◽

Jerzy Nawracała ◽

...

Keyword(s):

Multiplex Pcr ◽

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Field Resistance ◽

Wheat Cultivars ◽

Pcr Conditions ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

AbstractTen leading wheat cultivars originating from the Plant Breeding and Acclimatization Institute (IHAR) - National Research Institute (Poland) and the Department of Gene Bank (Czech Republic) were used to establish a field experiment in 2017 and 2018 at the Dłoń Experimental Farm. The analyzed wheat genotypes were characterized by diversified field resistance to leaf rust. Jubilatka, Thatcher and Sparta were the most resistant cultivars in field conditions in both 2017 and 2018. The aim of the work was to identify the Lr11, L13, Lr16 and Lr26 genes encoding resistance to leaf rust using molecular SSR markers (wmc24, wmc261, Xgwm630, Xwmc764 and P6M12) and to develop multiplex PCR conditions to accelerate identification of these genes. Markers of three leaf rust resistance genes have been identified simultaneously in these cultivars. Jubilatka, Thatcher and Sparta cultivars may serve as a good source of the analyzed leaf rust resistance genes. In addition, multiplex PCR conditions have been developed for the simultaneous identification of the Lr11 and Lr16 and Lr11 and Lr26 gene pairs.

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Postulation of seedling leaf rust resistance genes in 84 Chinese winter wheat cultivars

Journal of Integrative Agriculture ◽

10.1016/s2095-3119(14)61002-9 ◽

2015 ◽

Vol 14 (10) ◽

pp. 1992-2001 ◽

Cited By ~ 4

Author(s):

Xiao-li REN ◽

Tai-guo LIU ◽

Bo LIU ◽

Li GAO ◽

Wan-quan CHEN

Keyword(s):

Winter Wheat ◽

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Wheat Cultivars ◽

Winter Wheat Cultivars ◽

Seedling Leaf ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

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Development of wheat genotypes possessing a combination of leaf rust resistance genes Lr19 and Lr24

Plant Soil and Environment ◽

10.17221/4055-pse ◽

2011 ◽

Vol 50 (No. 10) ◽

pp. 434-438 ◽

Cited By ~ 3

Author(s):

S. Šliková ◽

E. Gregová ◽

P. Bartoš ◽

A. Hanzalová ◽

M. Hudcovicová ◽

...

Keyword(s):

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Null Allele ◽

Leaf Rust Resistance ◽

Resistance Testing ◽

Lr Genes ◽

Winter Wheat Cultivars ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

Endopeptidase allele Ep-D1c and DNA marker-assisted selection have been used for the incorporation of Lr19 + Lr24 leaf rust resistance genes combination into adapted commercial winter wheat cultivars. The first step was the transfer of the gene Lr19 from the donor cultivar Agrus into acceptor cultivars Simona and Lívia. The progenies possessing the null allele Ep-D1c linked to the gene Lr19 have been screened for their resistance to leaf rust by isolate 4332 SaBa. The plants homozygous properties at the Ep-D1c locus and resistant against leaf rust were used for crossing with NIL Thatcher/Lr24 – a donor of the gene Lr24. Plants possessing both Lr genes were selected from F2 population by STS and isozyme markers linked to the Lr genes. Progenies of 18 F2 plants have been selected by STS marker and tested for resistance against leaf rust. Results obtained with isozyme and STS markers corresponded with resistance testing. Altogether 6 progenies of F3 generation possessing a resistance gene combination of Lr19 + Lr24 in a homozygous condition were developed.

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Structural and functional analysis of genes with potential involvement in resistance to coffee leaf rust: a functional marker based approach

10.1101/763425 ◽

2019 ◽

Author(s):

Geleta Dugassa Barka ◽

Eveline Teixeira Caixeta ◽

Sávio Siqueira Ferreira ◽

Laércio Zambolim

Keyword(s):

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Function Analysis ◽

Bac Library ◽

Leaf Rust Resistance ◽

Molecular Data ◽

Coffee Leaf Rust ◽

Rust Resistance Genes

AbstractPhysiology-based differentiation of SH genes and Hemileia vastatrix races is the principal method employed for the characterization of coffee leaf rust resistance. Based on the gene-for-gene theory, nine major rust resistance genes (SH1-9) have been proposed. However, these genes have not been characterized at the molecular level. Consequently, the lack of molecular data regarding rust resistance genes or candidates is a major bottleneck in coffee breeding. To address this issue, we screened a BAC library with resistance gene analogs (RGAs), identified RGAs, characterized and explored for any SH related candidate genes. Herein, we report the identification and characterization of a gene (gene 11), which shares conserved sequences with other SH genes and displays a characteristic polymorphic allele conferring different resistance phenotypes. Furthermore, comparative analysis of the two RGAs belonging to CC-NBS-LRR revealed more intense diversifying selection in tomato and grape genomes than in coffee. For the first time, the present study has unveiled novel insights into the molecular nature of the SH genes, thereby opening new avenues for coffee rust resistance molecular breeding. The characterized candidate RGA is of particular importance for further biological function analysis in coffee.

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