scholarly journals In vitro propagation of Tilia platyphyllos by axillary shoot proliferation and somatic embryogenesis

2012 ◽  
Vol 49 (No. 12) ◽  
pp. 537-543 ◽  
Author(s):  
V. Chalupa
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
In Vitro Propagation ◽  
Shoot Proliferation ◽  
Tree Age ◽  
Nodal Segments ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation ◽  
Tilia Platyphyllos

In vitro propagation of Tilia platyphyllos Scop. has been achieved by axillary shoot proliferation and somatic embryo-<br />genesis. The influence of tree age, explant source, genotype, and phytohormones on micropropagation of juvenile and mature trees of Tilia platyphyllos has been investigated. Nodal segments and shoot tips were used as initial explants for axillary shoot proliferation. Low concentration of cytokinin (BA, BPA, TDZ) plus auxin (IBA) stimulated fast shoot multiplication. Microshoots<br />excised from proliferating cultures were rooted on low salt medium and produced trees were planted in the field. Embryo-<br />genic tissues were initiated from zygotic embryos cultured on MS medium supplemented with 2,4-D. After transfer of&nbsp; embryogenic tissues with developing embryoids on media lacking 2,4-D and supplemented with low concetration of IBA, the development of somatic embryos was enhanced. Secondary somatic embryogenesis led to the formation of new adventive somatic embryos. Trees produced from somatic embryos were planted in the field and exhibited normal growth and morphology.

scholarly journals Vegetative Propagation of Phytophthora cinnamomi-Tolerant Holm Oak Genotypes by Axillary Budding and Somatic Embryogenesis

Forests ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 841
Author(s):  
Maria Teresa Martínez ◽  
Francisco Javier Vieitez ◽  
Alejandro Solla ◽  
Raúl Tapias ◽  
Noelia Ramírez-Martín ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Phytophthora Cinnamomi ◽  
Shoot Proliferation ◽  
Holm Oak ◽  
Induction Medium ◽  
Axillary Shoot ◽  
Oak Decline ◽  
Shoot Cultures

Holm oak (Quercus ilex) is one of the most widely distributed tree species in the Mediterranean basin. High mortality rates have been observed in holm oak populations in the southwest of the Iberian Peninsula as a result of oak decline syndrome. Selection and propagation of genotypes tolerant to this syndrome could aid the restoration of affected areas. In this article, we report micropropagation and conservation procedures based on axillary budding and somatic embryogenesis (SE) of holm oak plants, selected for their tolerance to Phytophthora cinnamomi—the main biotic factor responsible for oak decline. Forced shoots were obtained from potted plants of eight different genotypes, and used as stock material to establish in vitro shoot proliferation cultures. Reliable shoot proliferation was obtained in seven out the eight genotypes established in vitro, whereas multiplication rates were genotype-dependent. The highest rooting rates were obtained by culturing shoots for 24 h or 48 h on rooting induction medium containing 25 mg L−1 indole-3-butyric acid, followed by transfer to medium supplemented with 20 µM silver thiosulphate. Axillary shoot cultures can be successful conserved by cold storage for 12 months at 4 °C under dim lighting. Shoot tips, excised from axillary shoot cultures established from tolerant plants, were used as initial explants to induce SE. Somatic embryos and/or nodular embryogenic structures were obtained on induction medium with or without indole-acetic acid 4 mg L−1, in two out the three genotypes evaluated, and induction rates ranged between 2 and 4%. Plantlet recovery was 45% after two months cold stratification of somatic embryos and eight weeks of culture on germination medium. Vegetative propagation of P. cinnamomi-tolerant Q. ilex trees is a valuable milestone towards the restoration of disease-affected areas.

scholarly journals In Vitro Propagation of Muscadine Grape by Axillary Shoot Proliferation

1990 ◽  
Vol 115 (2) ◽  
pp. 324-329 ◽  
Author(s):  
Ni Lee ◽  
Hazel Y. Wetzstein
Keyword(s):  
Basal Medium ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation ◽  
Vitis Rotundifolia ◽  
Shoot Production ◽  
Muscadine Grape ◽  
Better Than

Plantlets were recovered from axillary bud cultures of muscadine grape (Vitis rotundifolia, `Summit'). Nodal segments 0.5 to 1.0 cm long were cultured in Murashige and Skoog (MS) basal medium supplemented with 5, 10, 20, or 40 μm BA. Best total shoot production was obtained with 10 μm BA; with higher BA levels, shoots were unexpanded and exhibited high mortalities. MS medium supplemented with IBA enhanced rooting by increasing rooting percentage and number per plantlet. Shoots previously proliferated on medium with 5 μm BA rooted significantly better than those multiplied on 10 μM BA. Shoot vigor during rooting was greater in shoots proliferated on 5 vs. 10 μm BA. Root development was not significantly affected by liquid vs. agar-solidifted medium or shoot length. Chemical names used: N-(phenylmethyl) -1H-purin-6-amine (BA), 1H-indole-3-butyric acid (IBA).

scholarly journals In Vitro Regeneration of Vitex negundo L., a Woody Valuable Medicinal Plant through High Frequency Axillary Shoot Proliferation

1970 ◽  
Vol 43 (3) ◽  
pp. 345-352 ◽  
Author(s):  
Farhana Afroz ◽  
AKM Sayeed Hassan ◽  
Laila Shamroze Bari ◽  
Rebeka Sultana ◽  
John Liton Munshi ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
Axillary Shoot ◽  
Vitex Negundo ◽  
Axillary Shoot Proliferation

An efficient protocol was established for rapid and large scale propagation of woody aromatic medicinal plant Vitex negundo L. by in vitro shoot multiplication from shoot tips and nodal segments of mature plant. Of the four different growth regulators BA, Kn, GA3, NAA and coconut water, MS fortified with BA 1.0 mg/l was found to be the most effective for inducing multiple shoots from nodal explants. The percentage (96%) of shoot multiplication per node (21.83) was highest up to second subculture passages, after which there was a gradual decline in shoot development. Best rooting was induced (93%) in excised shoots on half strength MS medium supplemented with an optimal combination of NAA (0.3 mg/l). Soil, compost and sand (1:1:1) mixture was the most suitable planting substrate for hardening. The survival rate was 80% and the regenerated plants were successfully transferred to the soil.Key words: Vitex negundo, Medicinal plant, Shoot proliferation, Micropropagation, RegenerationDOI = 10.3329/bjsir.v43i3.1149Bangladesh J. Sci. Ind. Res. 43(3), 345-352, 2008

Standard Protocols for in Vitro Propagation of Bamboo with Emphasis on Axillary Shoot Proliferation

2021 ◽  
pp. 63-84
Author(s):  
Víctor M. Jiménez ◽  
Andrea Holst ◽  
Paula Carvajal-Campos ◽  
Eric Guevara
Keyword(s):  
In Vitro Propagation ◽  
Shoot Proliferation ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation

scholarly journals Efficient Axillary Shoot Proliferation and in Vitro Rooting of Apple cv. 'Topaz'

2012 ◽  
Vol 40 (1) ◽  
pp. 113 ◽  
Author(s):  
Snjezana KERESA ◽  
Anita MIHOVILOVIC BOSNJAK ◽  
Marijana BARIC ◽  
Ivanka HABUS JERCIC ◽  
Hrvoje SARCEVIC ◽  
...  
Keyword(s):  
Shoot Proliferation ◽  
Fruit Production ◽  
Nodal Segments ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation ◽  
Efficient Procedure ◽  
Plantlet Production ◽  
Organic Fruit

'Topaz' is a modern Czech apple cultivar well accepted by consumers and scab-resistant, providing reasons for the significant spreadof cv. 'Topaz' in European orchards, especially in the organic fruit production industry. Growing the apple trees on their own rootsprovides some advantages in comparison with grafted trees. Micropropagation is the method of choice for plantlet production for thispurpose as well as for the establishment of healthy mother stock trees as a source of scions. The efficiency of axillary shoot proliferationwas examined on four media differing in plant growth regulators and their concentrations, and from three explant types: intact ordecapitated and defoliated microshoots placed vertically and one-nodal segments placed horizontally. All media consisted of Quoirin and Lepoivre (QL) macroelements and Murashige and Skoog (MS) microelements. Furthermore, rooting efficiency on six different media/treatments was analyzed. Media with 1 mg/L 6-benzylaminopurine (BA) or BA (0.5 mg/L) + 1.5 mg/L kinetin (Kin) produced similarnumber of microshoots per inoculated one (2.5 and 2.4, respectively). Medium with 1 mg/L thidiazuron (TDZ) produced significantlyhigher number of shoots (3.6) but they were fasciated. Three different explant types also produced similar numbers of microshoots.High rooting efficiency (68.7%), a high number of roots per shoot (6.6) and the best quality of shoots were obtained in rooting mediumcontaining 2 mg/L of indole-3-butyric acid (IBA). An efficient method of shoot proliferation was established, and, since rooting was themost critical step, an efficient procedure for rooting apple cv. 'Topaz' was established.

In vitro propagation of Corymbia torelliana × C. citriodora (Myrtaceae) via cytokinin-free node culture

2007 ◽  
Vol 55 (4) ◽  
pp. 471 ◽  
Author(s):  
S. J. Trueman ◽  
D. M. Richardson
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Propagation ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Field Testing ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation ◽  
Free Node ◽  
Node Culture

Hybrids between Corymbia torelliana (F.Muell.) K.D.Hill & L.A.S.Johnson and C. citriodora subsp. variegata (F.Muell.) A.R.Bean & M.W.McDonald are used extensively to establish forestry plantations in subtropical Australia. Methods were developed for in vitro seed germination, shoot multiplication and plantlet formation that could be used to establish in vitro and ex vitro clone banks of juvenile Corymbia hybrids. Effects of sodium hypochlorite concentration and exposure time on seed contamination and germination, and effects of cytokinin and auxin concentrations on shoot multiplication and subsequent rooting, were assessed. A two-step surface sterilisation procedure, involving 70% ethanol followed by 1% sodium hypochlorite, provided almost no contamination and at least 88% germination. A novel method of cytokinin-free node culture proved most effective for in vitro propagation. Lateral bud break of primary shoots was difficult to induce by using cytokinin, but primary shoots rooted prolifically, elongated rapidly and produced multiple nodes in the absence of exogenous cytokinin. Further multiplication was obtained either by elongating lateral shoots of nodal explants in cytokinin-free medium or by inducing organogenic callus and axillary shoot proliferation with 2.2 µm benzyladenine. Plantlets were produced using an in vitro soil-less method that provided extensive rooting in sterile propagation mixture. These methods provide a means for simultaneous laboratory storage and field-testing of clones before selection and multiplication of desired genotypes.

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