Ultrosonogarphic and histological charac-teristics of the digestive tract in dogs with parvoviral enteritis

2020 ◽  
Vol 1 ◽  
pp. 115-123
Author(s):  
R.A. Tsyganski ◽  
◽  
A.N. Kvochko ◽  
V.V. Mikhailenko ◽  
◽  
...  

Since the clinical manifestation of enteri-tis of various etiologies has a similar picture, and parvovirus enteritis has a high mortality rate, early diagnosis of this disease is im-portant. A number of publications demon-strate changes in ultrasound parameters dur-ing inflammatory bowel diseases. The article is devoted to comparison of ultrasound and histological changes in the wall of the stom-ach and small intestine with parvovirus en-teritis in order to distinguish the most spe-cific ultrasound markers. Object of the study – 53 dogs of mixed breeds of both sexe with a confirmed diagnosis of parvovirus enteri-tis by polymerase chain reaction in real time from the age of 6 weeks to 7 months. Re-search were conducted at the Stavropol Vet-erinary Center named after Pirogov and Vet-erinary Center of the Stavropol State Agrari-an University, by the use of SIUI Apogee 1100 Omni scanner (Shantou Institute of Ultrasonic Instruments Co., Ltd., Guang-dong, China), according to the generally accepted method using a multi-frequency linear sensor with a frequency of 5-13 MHz in B-mode. The most characteristic ultrason- ic manifestations of canine parvovirus enteri-tis are hypotension and ectasia of the stom-ach and loops of the small intestine with the presence of anechogenic fluid content in their cavity; thickening of the surface layer of the mucosa in the form of a hyperechoic strip, a decrease in the ratio of the mucous layer to the entire wall thickness of the duo-denum and jejunum by more than 2 times and increased echogenicity of the mucous layer. This picture is a result of the partial necrosis and desquamation of villi, prolifera-tion of cell infiltrate in the mucous layer itself, consisting of lymphocytes, macro-phages, a small number of histiocytes and fibroblasts.

1995 ◽  
Vol 6 (7) ◽  
pp. 851-869 ◽  
Author(s):  
C L Wilson ◽  
K J Heppner ◽  
L A Rudolph ◽  
L M Matrisian

To explore the role of the matrix metalloproteinase matrilysin (MAT) in normal tissue remodeling, we cloned the murine homologue of MAT from postpartum uterus using RACE polymerase chain reaction and examined its pattern of expression in embryonic, neonatal, and adult mice. The murine coding sequence and the corresponding predicted protein sequence were found to be 75% and 70% identical to the human sequences, respectively, and organization of the six exons comprising the gene is similar to the human gene. Northern analysis and in situ hybridization revealed that MAT is expressed in the normal cycling, pregnant, and postpartum uterus, with levels of expression highest in the involuting uterus at early time points (6 h to 1.5 days postpartum). The mRNA was confined to epithelial cells lining the lumen and some glandular structures. High constitutive levels of MAT transcripts were also detected in the small intestine, where expression was localized to the epithelial Paneth cells at the base of the crypts. Similarly, MAT expression was found in epithelial cells of the efferent ducts, in the initial segment and cauda of the epididymis, and in an extra-hepatic branch of the bile duct. MAT transcripts were detectable only by reverse transcription-polymerase chain reaction in the colon, kidney, lung, skeletal muscle, skin, stomach, juvenile uterus, and normal, lactating, and involuting mammary gland, as was expression primarily late in embryogenesis. Analysis of MAT expression during postnatal development indicated that although MAT is expressed in the juvenile small intestine and reproductive organs, the accumulation of significant levels of MAT mRNA appears to correlate with organ maturation. These results show that MAT expression is restricted to specific organs in the mouse, where the mRNA is produced exclusively by epithelial cells, and suggest that in addition to matrix degradation and remodeling, MAT may play an important role in the differentiated function of these organs.


2009 ◽  
Vol 3 (1) ◽  
pp. 18
Author(s):  
E. Toporowska-Kowalska ◽  
B. Gebora-Kowalska ◽  
J. Kudzin ◽  
A. Plocek ◽  
E. Biernacka ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document