Summary In 1978 a 10 ha clonal seed orchard of black pine (Pinus nigra Arnold) was established in the area of Koumani in the western part of Peleponnesos, Greece. The orchard comprises 52 clones derived from intensively selected plus trees in the natural forest of black pine of Peloponnesos. In 1991 three open pollinated progeny tests were established in Peloponnesos, proximal to the villages of Raches, Vlachokerasia and Vamvakou. Seedlings from 52 families including a commercial check (CC) were planted in each one of the three locations. Assessments were made when the trees were 4, 7 and 9 years respectively, with the following results. The variation among families for growth characteristics were highly significant in all locations examined. In the combined analyses of variance over the three locations, significant differences among families were also detected, while the family x location interaction effect was not significant. This indicates that the seed produced from the seed orchard can be freely used over the environments of the three experimental plantings, which are representative of the broad area of Peloponnesos. Narrow sense heritability estimates on individual tree basis (h2) were variable depending on the characteristic, age of assessment and the location of the experimental plantings. The estimates of h2 in Vlachokerasia for height (HT) were 0.21, 0.40 and 0.43 at the ages of 4, 7 and 9 years respectively. In Raches the corresponding h2 values for HT were nearly the same in all ages (0.29, 0.28 and 0.31 at 4, 7 and 9 years respectively) and stable but little higher (0.31, 0.28 and 0.31) at the Vamvakou experimental planting. The heritability values for HT estimated over the three location, were relatively low (0.25, 0.23 and 0.19) at the ages 4, 7 and 9 years respectively. Realized genetic gains were calculated for growth characteristics at the age of 9 years, by comparing the performance of the improved (selected) materials to unimproved materials (CC). For the first stage of selection (selection made in natural stands) gain of 6.0% for HT, 8.0% for diameter breast height (DBH) and 24% for volume were estimated. When 20% of the clones, with the lower breeding values are removed from the seed orchard (genetic thinning), an additional gain of 2% for HT, 3% for DBH and 8% for volume over the unrogued seed orchard is resulted. Thus, the total genetic gain from the genetically tested, first generation seed orchard of black pine at Koumani is estimated as 8% for HT, 11% for DBH and 32% for volume. These results indicate that improvement of black pine by selection, establishment of seed orchard and progeny testing the clones, is a very promising profitable operation.
Effective Clone Number and Contribution to Gene Pool in a Clonal Seed Orchard of Anatolian Black Pine (Pinus nigra Arnold subsp. pallasiana (Lamb.) Holmboe) in Kastamonu-Turkey