Abstract
This research was carried out to investigate the effects of chemical mutagen applications on producing phenolic compounds in calli obtained from leaf petiole explants of the Royal grape variety.Petioles were cultured in B5 medium containing 0.5 mg/L benzyl amino purine and 0.5 mg/L indole acetic acid to obtain callus. Calli obtained during the period following planting were subcultured in the same culture conditions three times at one month intervals. In the fourth subculture, the calli were transferred to nutrient mediums with the same composition containing ethyl methanesulfonate, sodium azide, azacitidine and acridine orange in three different doses, 0.5 mM, 1.0 mM, and 2.0 mM, and cultured in these mediums for 60, 120 and 180 minutes. Then, they were cultured again for four weeks in mutagen-free nutrient mediums with the same content as their previous mediums. At the end of this period, calli were taken from their nutrient medium, and total phenolic compound, total flavanol, total flavonol and anthocyanin analyzes were performed.As a result of the research, it has been determined that all mutagens applied are effective in increasing the production of phenolic compounds depending on the dose and time.
575 PB 050 EFFICIENT PLANT REGENERATION FROM LEAF AND PETIOLE EXPLANTS IN RED RASPBERRY
