Agrobacterium-Mediated Transformation Of Plant Cells

2018 ◽  
Author(s):  
Christine Kronfoth ◽  
◽  
Peter Grayson ◽  
Keyword(s):  
Plant Cells ◽  
Agrobacterium Mediated Transformation

scholarly journals Homologous recombination in plant cells after Agrobacterium-mediated transformation.

1990 ◽  
Vol 2 (5) ◽  
pp. 415-425 ◽  
Author(s):  
K Y Lee ◽  
P Lund ◽  
K Lowe ◽  
P Dunsmuir
Keyword(s):  
Homologous Recombination ◽  
Plant Cells ◽  
Agrobacterium Mediated Transformation

scholarly journals Homologous Recombination in Plant Cells after Agrobacterium-Mediated Transformation

1990 ◽  
Vol 2 (5) ◽  
pp. 415 ◽  
Author(s):  
Kathleen Y. Lee ◽  
Peter Lund ◽  
Keith Lowe ◽  
Pamela Dunsmuir
Keyword(s):  
Homologous Recombination ◽  
Plant Cells ◽  
Agrobacterium Mediated Transformation

scholarly journals Extrachromosomal homologous recombination and gene targeting in plant cells after Agrobacterium mediated transformation.

1990 ◽  
Vol 9 (10) ◽  
pp. 3077-3084 ◽  
Author(s):  
R. Offringa ◽  
M. J. de Groot ◽  
H. J. Haagsman ◽  
M. P. Does ◽  
P. J. van den Elzen ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Gene Targeting ◽  
Plant Cells ◽  
Agrobacterium Mediated Transformation

scholarly journals Overexpression of the HspL Promotes Agrobacterium tumefaciens Virulence in Arabidopsis Under Heat Shock Conditions

2015 ◽  
Vol 105 (2) ◽  
pp. 160-168 ◽  
Author(s):  
Hau-Hsuan Hwang ◽  
Yin-Tzu Liu ◽  
Si-Chi Huang ◽  
Chin-Yi Tung ◽  
Fan-Chen Huang ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Heat Shock ◽  
Transformation Efficiency ◽  
Plant Cells ◽  
Small Heat Shock Protein ◽  
Protein Accumulation ◽  
Transient Transformation ◽  
Root Explants ◽  
Agrobacterium Mediated Transformation ◽  
The Impact

Agrobacterium tumefaciens transfers a specific DNA fragment from the resident tumor-inducing (Ti) plasmid and effector virulence (Vir) proteins to plant cells during infection. A. tumefaciens VirB1-11 and VirD4 proteins assemble as the type IV secretion system (T4SS), which mediates transfer of the T-DNA and effector Vir protein into plant cells, thus resulting in crown gall disease in plants. Previous studies revealed that an α-crystallin-type, small heat-shock protein (HspL) is a more effective VirB8 chaperone than three other small heat-shock proteins (HspC, HspAT1, and HspAT2). Additionally, HspL contributes to efficient T4SS-mediated DNA transfer and tumorigenesis under room-temperature growth. In this study, we aimed to characterize the impact of HspL on Agrobacterium-mediated transformation efficiency under heat-shock treatment. During heat shock, transient transformation efficiency and VirB8 protein accumulation were lower in the hspL deletion mutant than in the wild type. Overexpression of HspL in A. tumefaciens enhanced the transient transformation efficiency in root explants of both susceptible and recalcitrant Arabidopsis ecotypes. In addition, the reduced transient transformation efficiency during heat stress was recovered by overexpression of HspL in A. tumefaciens. HspL may help maintain VirB8 homeostasis and elevate Agrobacterium-mediated transformation efficiency under both heat-shock and nonheat-shock growth.

SEM study of the morphological effects of kinetin and zeatin on the growth and development of the apical meristem in wheat

1995 ◽  
Vol 53 ◽  
pp. 978-979
Author(s):  
G. M. Hutchins ◽  
J. S. Gardner
Keyword(s):  
Growth And Development ◽  
Furfuryl Alcohol ◽  
Apical Meristem ◽  
Plant Cells ◽  
Triticum Aestivum L ◽  
Morphological Development ◽  
Naturally Occurring ◽  
Chinese Spring Wheat ◽  
Sem Study ◽  
Moist Environment

Cytokinins are plant hormones that play a large and incompletely understood role in the life-cycle of plants. The goal of this study was to determine what roles cytokinins play in the morphological development of wheat. To achieve any real success in altering the development and growth of wheat, the cytokinins must be applied directly to the apical meristem, or spike of the plant. It is in this region that the plant cells are actively undergoing mitosis. Kinetin and Zeatin were the two cytokinins chosen for this experiment. Kinetin is an artificial hormone that was originally extracted from old or heated DNA. Kinetin is easily made from the reaction of adenine and furfuryl alcohol. Zeatin is a naturally occurring hormone found in corn, wheat, and many other plants.Chinese Spring Wheat (Triticum aestivum L.) was used for this experiment. Prior to planting, the seeds were germinated in a moist environment for 72 hours.

Confocal microscopy of the cytoskeleton in living plant cells following microinjection of fluorescent probes

1993 ◽  
Vol 51 ◽  
pp. 130-131
Author(s):  
Ann Cleary
Keyword(s):  
Cell Division ◽  
Fluorescent Probes ◽  
Actin Filaments ◽  
Intracellular Transport ◽  
Cell Structure ◽  
Plant Cells ◽  
Cell Plate ◽  
Cell Cortex ◽  
Living Plant ◽  
Rhodamine Phalloidin

Microinjection of fluorescent probes into living plant cells reveals new aspects of cell structure and function. Microtubules and actin filaments are dynamic components of the cytoskeleton and are involved in cell growth, division and intracellular transport. To date, cytoskeletal probes used in microinjection studies have included rhodamine-phalloidin for labelling actin filaments and fluorescently labelled animal tubulin for incorporation into microtubules. From a recent study of Tradescantia stamen hair cells it appears that actin may have a role in defining the plane of cell division. Unlike microtubules, actin is present in the cell cortex and delimits the division site throughout mitosis. Herein, I shall describe actin, its arrangement and putative role in cell plate placement, in another material, living cells of Tradescantia leaf epidermis.The epidermis is peeled from the abaxial surface of young leaves usually without disruption to cytoplasmic streaming or cell division. The peel is stuck to the base of a well slide using 0.1% polyethylenimine and bathed in a solution of 1% mannitol +/− 1 mM probenecid.

Scanning electron microscopy of plant cells using a variable-pressure SEM and cryogenic techniques

1993 ◽  
Vol 51 ◽  
pp. 260-261
Author(s):  
M. Yamada ◽  
K. Ueda ◽  
K. Kuboki ◽  
H. Matsushima ◽  
S. Joens
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Saturated Vapor ◽  
Plant Cells ◽  
Variable Pressure ◽  
Specimen Preparation ◽  
Operating Pressure ◽  
Vapor Pressures ◽  
Low Temperature Microscopy ◽  
Scanning Electron

Use of variable Pressure SEMs is spreading among electron microscopists The variable Pressure SEM does not necessarily require specimen Preparation such as fixation, dehydration, coating, etc which have been required for conventional scanning electron microscopy. The variable Pressure SEM allows operating Pressure of 1˜270 Pa in specimen chamber It does not allow microscopy of water-containing specimens under a saturated vapor Pressure of water. Therefore, it may cause shrink or deformation of water-containing soft specimens such as plant cells due to evaporation of water. A solution to this Problem is to lower the specimen temperature and maintain saturated vapor Pressures of water at low as shown in Fig. 1 On this technique, there is a Published report of experiment to have sufficient signal to noise ratio for scondary electron imaging at a relatively long working distance using an environmental SEM. We report here a new low temperature microscopy of soft Plant cells using a variable Pressure SEM (Hitachi S-225ON).

Efficient initiation of translation at non-AUG triplets in plant cells.

1992 ◽  
Vol 2 (5) ◽  
pp. 809-813 ◽  
Author(s):  
K Gordon ◽  
J Futterer ◽  
T Hohn
Keyword(s):  
Plant Cells ◽  
Initiation Of Translation
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