scholarly journals The Effect of MS Media Strength and Cytokinin in the Induction of Shoots from Shoot Tip Explants of Australian Finger Lime (Citrus australasica cv. Tasty Green)

2021 ◽  
Vol 50 (5) ◽  
pp. 1277-1284
Author(s):  
Dahmendra Sriskanda ◽  
Siong Poi Khoy ◽  
Najwa Amalina Haradzi ◽  
Nurulhikma Md Isa ◽  
Sreeramanan Subramaniam ◽  
...  
Keyword(s):  
Kidney Stones ◽  
East Coast ◽  
Shoot Elongation ◽  
Shoot Length ◽  
Shoot Tip ◽  
Cell Protection ◽  
Axillary Shoots ◽  
Full Strength ◽  
Half Strength ◽  
Shoot Tip Explants

The Australian Finger Lime (Citrus australasica) is a type of citrus from the Rutaceae family, endemic to the east coast of Australia. The finger lime, loaded with numerous vitamins and renders a unique taste, has also been backed by science to contain essential amounts of antioxidants that are beneficial for cell protection, immune response, cancer prevention, ageing, arthritis and prevention of kidney stones. Current propagation attempts still rely on conventional methods that are less efficient and resulted in the slow establishment of farms for finger lime especially for commercialization purposes. This study focuses on the induction of shoots from shoot tip explants using 6-Benzylaminopurine (BAP) and Kinetin. Aseptic explants were inoculated into Murashige and Skoog (MS) medium of full-strength and half-strength followed by full-strength MS media supplemented with different concentrations of BAP and Kinetin. Results obtained in this study showed no significant differences in terms of the number of axillary shoots produced between explants cultured in full and half-strength MS media. However, the highest number of shoots and increment in shoot length were obtained from MS media supplemented with 2.0 mg/L BAP with the values 1.80 ± 0.27 and 2.56 ± 0.36 cm, respectively. In conclusion, MS media supplemented with 2.0 mg/L BAP was found optimal in the induction of shoots and shoot elongation of C. australasica cv. Tasty Green.

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

scholarly journals Micropropagation of Members of the Cactaceae Subtribe Cactinae

1990 ◽  
Vol 115 (2) ◽  
pp. 337-343 ◽  
Author(s):  
Philip W. Clayton ◽  
John F. Hubstenberger ◽  
Gregory C. Phillips ◽  
S. Ann Butler-Nance
Keyword(s):  
Shoot Proliferation ◽  
Shoot Tip ◽  
Axillary Shoot ◽  
Axillary Shoots ◽  
Axillary Shoot Proliferation ◽  
Shoot Production ◽  
Series Of Experiments ◽  
Basal Media ◽  
Free Media ◽  
Shoot Tip Explants

Micropropagation of 11 rare or endangered cacti species belonging to the subtribe Cactinae was achieved by rooting of proliferated axillary shoots. Shoot tip explants were obtained from seedlings of Escobaria missouriensis D.R. Hunt, E. robbinsorum (Earle) D.R. Hunt, Sclerocactus spinosior (Engelm.) Woodruff & L. Benson, and Toumeya papyracantha (Engelm.) Br. & Rose, and from mature plants of Mammillaria wrightii Engelm., Pediocactus bradyi L. Benson, P. despainii Welsh & Goodrich, P. knowltonii L. Benson, P. paradinei B.W. Benson, P. winkleri Heil, and S. mesae-verdae (Boissevain) L. Benson. Three or four species were used in each of a series of experiments investigating the effects of basal media and auxin and cytokinin types and concentrations on axillary shoot proliferation. Low or no auxin but moderate to high cytokinin concentrations were required for axillary shoot production. All species rooted spontaneously on hormone-free media; however, several species rooted better on media containing auxin. All species were re-established in the greenhouse.

Nutrient responses differ between node and organogenic cultures of Corymbia torelliana×C. citriodora (Myrtaceae)

2010 ◽  
Vol 58 (5) ◽  
pp. 410 ◽  
Author(s):  
Cao Dinh Hung ◽  
Stephen J. Trueman
Keyword(s):  
Root Formation ◽  
Shoot Proliferation ◽  
Shoot Elongation ◽  
Plantation Establishment ◽  
Full Strength ◽  
Eastern Australia ◽  
Half Strength ◽  
Strength Medium ◽  
Node Culture

Hybrids between Corymbia torelliana (F.Muell.) K.D.Hill & L.A.S.Johnson and C. citriodora subsp. variegata (F.Muell.) A.R.Bean & M.W.McDonald are used extensively for plantation forestry in subtropical eastern Australia; however, plantation establishment has been hampered by inadequate seed supply and variable amenability to propagation as rooted cuttings. The present study investigated node-culture and organogenic-culture methods for in vitro propagation of two families of juvenile Corymbia hybrids. The effects of nutrient and auxin concentrations on root formation, shoot elongation and shoot proliferation, and subsequent conversion to plantlets in an in vitro soil-less system, were assessed. The response to the nutrient concentration differed between organogenic and node cultures. Half-strength, compared with full-strength, medium with auxin increased shoot proliferation by 58% for one family in the node culture, in which many shoots form roots and elongate rapidly in the absence of cytokinin. However, full-strength medium increased shoot proliferation by 213% over that in the half-strength medium for the other family in the organogenic culture, in which shoots do not form roots in the presence of cytokinin, and nutrient uptake occurs via callus and stem tissue rather than roots. The auxin, naphthalene acetic acid, sometimes stimulated root formation and shoot elongation in the node culture, although it had no effect on the final number of shoots. Most shoots (57–100%) subsequently formed roots in the in vitro soil-less medium. The high rates of proliferation and plantlet conversion in the present study provide the means, not only for simultaneous laboratory storage and field-testing of Corymbia clones before selection of desired genotypes, but also for en masse plantlet production of selected clones or families for plantation establishment.

scholarly journals In vitro propagation of Andrographis paniculata Nees.-A threatened medicinal plant of Bangladesh

2016 ◽  
Vol 3 (1) ◽  
pp. 67-73
Author(s):  
PK Roy
Keyword(s):  
Activated Charcoal ◽  
In Vitro Regeneration ◽  
Shoot Elongation ◽  
Shoot Tip ◽  
Andrographis Paniculata ◽  
Nodal Segment ◽  
Threatened Medicinal Plant ◽  
Half Strength ◽  
Number Of Shoots

An efficient protocol was developed for in vitro regeneration of plantlets from shoot tip and nodal segment explants of Andrographis paniculata Nees. Nodal segment explants produced the highest number of shoots (18±1.24) when they were cultured on MS supplemented with 11.10 ?M/l BAP. Addition of 10% coconut water and 2.0 g/l activated charcoal to the above mentioned medium increased the number of shoots (30) per culture. Shoot tip explant also showed better performance in the same medium. Addition of 100 mg/l urea and 2.0 g/l activated charcoal to the medium showed proper shoot elongation. The isolated shoots rooted well (90%) on half-strength MS fortified with 9.80 ?M/l IBA, where average number of roots per shoot was 28-30. The plantlets were acclimatized successfully in poly bags containing a mixture of soil, sand and compost in 2:1:1 ratio. Finally acclimatized plantlets were transferred to experimental field.Jahangirnagar University J. Biol. Sci. 3(1): 67-73, 2014 (June)

scholarly journals In vitro Plant Regeneration from Shoot tip Explants of Exacum travancoricum Beedi

1970 ◽  
Vol 20 (2) ◽  
pp. 113-118 ◽  
Author(s):  
B. Janarthanam ◽  
E. Sumathi Sumathi
Keyword(s):  
Plant Regeneration ◽  
Growth Response ◽  
Average Length ◽  
Shoot Tip ◽  
Average Height ◽  
In Vitro Plant Regeneration ◽  
Farm Yard Manure ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants of Exacum travancoricum, an endangered herb mainly known for ornamental purpose grown on MS with 4.44 μM BAP and 1.34 μM NAA showed better growth response and produced 29.3 ± 0.3 shoots per explant with an average length of 4.6 ± 0.1 cm after 35 days. Roots were induced after transfer to half strength of MS supplemented with 2.46 μM IBA produced 4.8 ± 0.62 roots with an average height of 3.6 ± 0.10 cm after 30 days. The rooted plantlets were transferred for hardening, 80 per cent of plantlets survived and resumed growth in the mixture of soil, vermiculite and farm yard manure (1 : 1 : 1). Key words: Exacum travancoricum; Shoot tip; Plant regeneration D.O.I. 10.3329/ptcb.v20i2.6889 Plant Tissue Cult. & Biotech. 20(2): 113-118, 2010 (December)

scholarly journals Axillary Shoots Derived from Thin Cell Layer and Adenine Sulphate Application in in vitro Mass Propagation of Gerbera [Gerbera jamesonii (H. Bolus ex Bolus f.)]

2019 ◽  
Vol 11 (1) ◽  
pp. 63-76
Author(s):  
Budi WINARTO ◽  
Kurnia YUNIARTO ◽  
Dan M. WEGADARA
Keyword(s):  
Optimal Combination ◽  
Shoot Tip ◽  
Basic Medium ◽  
Ms Medium ◽  
Gerbera Jamesonii ◽  
Mass Propagation ◽  
Adenine Sulphate ◽  
Half Strength ◽  
Shoot Tip Explants

A new route of in vitro mass propagation protocol of Gerbera jamesonii (H. Bolus ex Bolus f.) derived from application of thin cell layers (TCL) and adenine sulphate (AS) was successfully developed and established. Shoot tip explants and half-strength MS medium containing 0.25 mg/l N6-benzylaminopurine (BAP), 20 g/l sucrose and 7 g/l Swallow agar were used as explant source and basic medium. Different TCL of transversal TCL (tTCL) and longitudinal TCL (lTCL) in four slicing positions of 1, 2, 3 and 4; varieties and clones i.e. G. jamesonii ‘Black Jack’, ‘Carambole’, ‘Nuance’, ‘Violente’, 01.098 and 11.46 clone; AS concentrations viz. 0, 20, 40, 60, 80 and 100 mg/l were tested in the study. Each step of in vitro culture established had unique and specific results. In the initiation stage, first slicing position of ‘Black Jack’ shoot tip tTCL was the most optimal combination treatment to produce 7.0 shoots per explant with 13.5 leaves. The first slicing position on shoot tip explants of 01.098 clone tTCL and 20 mg/l AS in half-strength MS medium containing 0.25 mg/l BAP were the most optimal combination treatment in obtaining the highest number of shoots produced per shoot up to 9.4 shoots per shoot with 34.1 leaves and 2.37 cm length of leaves in the proliferation stage, however the treatment did not give significant effect compared to control. Under periodical subcultures on the basic medium, number of shoots and leaves increased gradually from the initial culture with 3-6 shoots per shoot and 9.4-11.6 leaves till the fourth subculture with 6-11 shoots per shoot and 16.7-28.8 leaves and declined thereafter. Subculturing of shoots in accordance to produce qualified shoots for planting materials could be carried out till sixth to seventh subculture. The highest shoot multiplication rate (SMR) was established on 01.098 clone with as high as 7.3. The well shoots were easily rooted on half-strength MS medium supplemented with 0.1 mg/l BAP, 0.05 mg/l NAA and 1.5 g/l AC. Plantlets were then transferred to ex vitro condition for acclimatization on a mixture of burned-rice husk and organic manure (1:1, v/v) with 85-100% survivability. The ‘Black Jack’ and 11.46 clone were the best genotypes on the acclimatization stage with 100% survivability of plantlets. Results of the study have implication that first slicing position of shoot tip tTCL can be applied in establishing of in vitro propagation protocol for other gerberas.

scholarly journals Micropropagation of Antelope Bitterbrush [Purshia tridentata (Pursh) DC.]

HortScience ◽  
1997 ◽  
Vol 32 (2) ◽  
pp. 312-314 ◽  
Author(s):  
John L. Edson ◽  
David L. Wenny ◽  
Annette Leege-Brusven
Keyword(s):  
Woody Plant ◽  
Naphthaleneacetic Acid ◽  
Ex Vitro ◽  
Indolebutyric Acid ◽  
Axillary Shoots ◽  
Full Strength ◽  
Woody Plant Medium ◽  
Half Strength ◽  
In Vitro Survival

In vitro—derived microshoots of antelope bitterbrush, incubated for 1 month in media supplemented with 0.44 μm BA, grew 0.8 and 1.1 cm longer in woody plant medium (WPM) compared to full-strength and half-strength Murashige and Skoog (MS) media, respectively. Explants cultured in WPM supplemented with 0.44 μm BA and 0.54 μm NAA produced a mean of five axillary shoots per explant. Explants dipped in 0.1% IBA or 0.1% NAA rooted best in 0.1% IBA with 89% success ex vitro vs. 60% success in vitro. Survival of acclimatized plantlets rooted ex vitro was 95%, while 50% survived when rooted in vitro. After 1 year of greenhouse growth, 98% of plantlets survived and flowered. Chemical names used: benzyladenine (BA), 3-indolebutyric acid (IBA), 1-naphthaleneacetic acid (NAA).

scholarly journals In vitro Shoot Multiplication of Bupleurum disticho-phyllum Wight - A Native Medicinal Plant of Southern India

1970 ◽  
Vol 17 (2) ◽  
pp. 115-124 ◽  
Author(s):  
S. Karuppusamy ◽  
T. Pullaiah
Keyword(s):  
Medicinal Plant ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Shoot Tip ◽  
Axillary Shoot ◽  
Axillary Shoots ◽  
Axillary Shoot Proliferation ◽  
Shoot Tip Explants

Shoot multiplication of Bupleurum distichophyllum was achieved from the nodal and shoot tip explants of mature plants using MS with different concentrations and combinations of growth regulators. Maximum explant response was from axillary shoots and the highest number of shoots per explant was obtained on MS fortified with 1.0 mg/l BAP. The highest degree of axillary shoot proliferation was found to be 74 and 70% for nodal- and shoot tip explants, respectively on the medium containing 1.0 mg/l BAP + 0.1 mg/l NAA. The combination of BAP and GA3 was also found to be effective for both type of explants. The degree of shoot formation was affected by explant types and the exogenous hormonal regime in the medium. The regenerated shoots were successfully rooted on MS supplemented with 2.0 mg/l IBA, after sequential hardening, survival rate was 71%. Key words: Bupleurum distichophyllum, Medicinal plant, Micropropagation, Conservation Plant Tissue Cult. & Biotech. 17(2): 115-124, 2007 (December) DOI: 10.3329/ptcb.v17i2.2574

scholarly journals In Vitro Propagation and NaCl Tolerance of the Multipurpose Medicinal Halophyte Limoniastrum monopetalum

HortScience ◽  
2020 ◽  
Vol 55 (4) ◽  
pp. 436-443
Author(s):  
Aikaterini N. Martini ◽  
Maria Papafotiou
Keyword(s):  
Salt Marshes ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Shoot Length ◽  
In Vitro Rooting ◽  
Ms Medium ◽  
Perennial Shrub ◽  
Half Strength ◽  
Limoniastrum Monopetalum

Limoniastrum monopetalum is an evergreen perennial shrub native to Mediterranean coastal sands and salt marshes. It has adapted to a variety of environmental stresses and is used in traditional medicine and as an ornamental plant. In the present study, an efficient micropropagation protocol for this species was developed to facilitate the production of selected genotypes and promote its wider use. Research has focused on the effects of various cytokinin types [benzyladenine (BA), zeatin, 6-furfurylaminopurine (kinetin) or 6-γ-γ-dimethylallilopurine (2iP)] and concentrations (0.0–4.0 mg·L−1) and various NaCl concentrations (0.0–20 g·L−1) during all stages of in vitro culture. For in vitro establishment, Murashige and Skoog (MS) medium supplemented with 0.5 mg·L−1 BA and 0.0 or 5.0 g·L−1 NaCl was most appropriate (100% explant response, 3–4 shoots per explant, 2 cm shoot length). The best results for shoot multiplication (100% response, 9 shoots per explant, 0.8–1.0 cm shoot length) were obtained with low (0.5 mg·L−1) BA or relatively high (2.0 mg·L−1) kinetin concentrations in the medium; however, 0.5 mg·L−1 kinetin should be preferred in the case of production of multiple rooted microshoots during one stage. The addition of NaCl at relatively low concentrations (2.5 or 5.0 g·L−1) in a medium supplemented with 0.5 mg·L−1 BA doubled shoot multiplication but did not improve shoot elongation (100% explant response, 16 shoots per explant, 0.8 cm shoot length). For in vitro rooting, half-strength MS medium supplemented with 1.0 mg·L−1 IBA was most appropriate (97% rooting, 9.4 roots per microshoot, 1.2 cm root length). Regarding the effects of NaCl on in vitro rooting, microshoots were relatively tolerant to NaCl concentrations up to 10.0 g·L−1. The effects of NaCl depend on the micropropagation stage; they are synergistic during shoot multiplication and tolerant during rooting. However, explants responded satisfactorily in its absence, indicating that NaCl was not necessary as a medium component. Ex vitro acclimatization and establishment of plantlets was 100% successful in a mixture of peat:perlite 1:1 or 2:1 (v/v).

Evaluation of explants for in vitro propagation of Citrus indica Tanaka - An Endangered Species

2020 ◽  
Vol 30 (1) ◽  
pp. 87-96
Author(s):  
Shabaree YA Sangma ◽  
Lolly S Pereira ◽  
JC Dang ◽  
Binu Mathew
Keyword(s):  
Leaf Disc ◽  
Shoot Length ◽  
Shoot Tip ◽  
Root Tip ◽  
Nodal Segment ◽  
Ms Medium ◽  
Shoot Bud ◽  
Shoot Number ◽  
Half Strength

Of the five explants viz., shoot tip, nodal segment, leaf disc, cotyledon and root tip excised from exegenic seedlings of Citrus indica Tanaka shoot tip cultured in MS supplemented with 0.5 mg/l of BAP generated shoots within 4.74 days, exhibiting highest percentage of response (85.82%) with highest number of shoots (8.9) and shoot length (3.04 cm). On the other hand nodal segment cultured in MS supplemented with 1.0 mg/l BAP showed 80% response in 5.16 days with a shoot number of 5.41 and shoot length of 2.43 cm. Cotyledon explants inoculated on MS supplemented with1.0 mg/l of TDZ produced shoots in 20 days with the highest response of 69.88%, with 3.77 shoots per cotyledon and shoot length of 2.03cm. Viable callus was obtained from leaf disc cultured on half strength MS medium with less Ca++ with 2, 4-D 0.5 mg/l + Kn 0.25 mg/l. This callus when inoculated on half strength MS medium with Kn 1.5 mg/l showed highest shoot bud proliferation of 66.66% with 10.06 shoots per callus. Root tip explant failed to produce any shoots. In vitro raised shoots of Citrus indica when cultured on half strength MS medium supplemented with NAA (1.0 mg/l) showed 80 % rooting in 5.66 days, with highest number of roots (6.16 per shoot) and longest root (3.78cm). Ninety per cent of in vitro rooted plantlets of Citrus indica survived in open conditions. Plant Tissue Cult. & Biotech. 30(1): 87-96, 2020 (June)

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