DNA FINGERPRINTING IN HYDRASTIS CANADENSIS USING RAPD ANALYSIS

DNA fingerprints of 64 potato cultivars that are commercially grown in Australia were generated using PCR-based RAPD analysis. All 64 cultivars were differentiated by banding patterns obtained from 17 primers that generated 133 polymorphisms. Clonal variants of cvv. Atlantic, Kennebec, Sebago, and Russet Burbank were found to have within-cultivar identical banding patterns. The largest genetic similarity between potato cultivars and the Solanum andigena and Solanum acuale outgroups were 0.5 and 0.4, respectively. The genetic similarity between only potato cultivars ranged from 0.67 to 0.9. Using similarity data a dendrogram was constructed that showed close genetic relationships between a number of cultivars of similar pedigree. This study has shown that DNA fingerprinting is a useful tool for potato cultivar identification, differentiation, and estimating genetic relationships.

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Enterotoxicity and genetic variation among clinical Staphylococcus aureus isolates in Jordan

Journal of Medical Microbiology ◽

10.1099/jmm.0.46183-0 ◽

2006 ◽

Vol 55 (2) ◽

pp. 183-187 ◽

Cited By ~ 29

Author(s):

Randa G. Naffa ◽

Salwa M. Bdour ◽

Hussein M. Migdadi ◽

Asem A. Shehabi

Keyword(s):

Genetic Diversity ◽

Staphylococcus Aureus ◽

Genetic Variation ◽

Multiplex Pcr ◽

Dna Fingerprinting ◽

Rapd Analysis ◽

Clinical Isolates ◽

Randomly Amplified Polymorphic Dna ◽

Toxin Genes ◽

Enterotoxin Genes

A total of 100 Jordanian clinical Staphylococcus aureus isolates was analysed for the presence of the enterotoxin genes sea, seb, sec, sed and see using multiplex PCR. Twenty-three isolates (23 %) were potentially enterotoxigenic. The prevalence of sea, sec and sea plus sec among the total clinical isolates was 15, 4 and 4 %, respectively. None of the isolates harboured sed, seb or see genes. S. aureus isolates were subjected to DNA fingerprinting by randomly amplified polymorphic DNA (RAPD) analysis to test whether isolates harbouring the toxin genes were genetically clustered. A total of 13 genotypes was identified at a 47 % similarity level. Genotypes I and V accounted for the largest number of enterotoxigenic isolates (19 %). This study has demonstrated the genetic diversity of Jordanian clinical S. aureus isolates and shown that the presence of the toxin genes is not genotype specific.

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A measure of genetic diversity of goldenseal (Hydrastis canadensis L.) by RAPD analysis

Genetic Resources and Crop Evolution ◽

10.1007/s10722-013-9962-7 ◽

2013 ◽

Vol 60 (3) ◽

pp. 1201-1207

Author(s):

Maiko Inoue ◽

Kerry J. Kelley ◽

Amy Frary ◽

Lyle E. Craker

Keyword(s):

Genetic Diversity ◽

Rapd Analysis ◽

Hydrastis Canadensis

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Genetic Characterization by RAPD Analysis of Isolates of Fusarium oxysporum f. sp. erythroxyli Associated with an Emerging Epidemic in Peru

Phytopathology ◽

10.1094/phyto.1997.87.12.1220 ◽

1997 ◽

Vol 87 (12) ◽

pp. 1220-1225 ◽

Cited By ~ 26

Author(s):

Amy J. Nelson ◽

Karol S. Elias ◽

E. Arévalo G. ◽

Lee C. Darlington ◽

Bryan A. Bailey

Keyword(s):

Fusarium Oxysporum ◽

Dna Fingerprinting ◽

Genetic Characterization ◽

Rapd Analysis ◽

Random Amplified Polymorphic Dna ◽

Dna Fingerprint ◽

Pathogenicity Test ◽

Vascular Wilt ◽

Genetic Complexity ◽

Rapd Pattern

An epidemic of vascular wilt caused by Fusarium oxysporum f. sp. erythroxyli is currently occurring on Erythroxylum coca var. coca in the coca-growing regions of the Huallaga Valley in Peru. Random amplified polymorphic DNA (RAPD) analysis of isolates of the pathogen was undertaken to elucidate its genetic complexity, as well as to identify a specific DNA fingerprint for the pathogen. Two hundred isolates of Fusarium were collected from 10 coca-growing regions in Peru. Of these, 187 were confirmed to be F. oxysporum, and 143 of the F. oxysporum were shown to be pathogens of coca by a root-dip pathogenicity test. The pathogens could be grouped into two subpopulations based on RAPD analysis, and no polymorphism in RAPD pattern was observed among isolates of either subpopulation. Both subpopulations were present in the central Huallaga Valley, where earliest reports of the epidemic occurred. RAPD analysis could easily distinguish the isolates of F. oxysporum f. sp. erythroxyli from the nonpathogenic isolates of F. oxysporum from E. coca var. coca, indicating its utility in DNA fingerprinting.

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