MICROARRAY CHARACTERIZATION OF THE HRPL REGULON OF THE FIRE BLIGHT PATHOGEN ERWINIA AMYLOVORA

2011 ◽  
pp. 263-270
Author(s):  
R.R. McNally ◽  
G.W. Sundin ◽  
Y.F. Zhao ◽  
I.K. Toth ◽  
P.J.A. Cock ◽  
...  
Keyword(s):  
Fire Blight ◽  
Erwinia Amylovora

Isolation and Molecular Characterization of the Fire Blight Pathogen, Erwinia amylovora, Isolated from Apple and Pear Orchards in Egypt

2015 ◽  
Vol 14 (3) ◽  
pp. 142-147 ◽  
Author(s):  
Nader A. Ashmawy ◽  
Taha I. Zaghlou ◽  
Moustafa A. El-Saba
Keyword(s):  
Molecular Characterization ◽  
Fire Blight ◽  
Erwinia Amylovora

scholarly journals Structural and functional characterization of proteins from the fire blight pathogen Erwinia amylovora. A review on the state of the art

2020 ◽  
Author(s):  
Stefano Benini
Keyword(s):  
Fire Blight ◽  
Erwinia Amylovora ◽  
Functional Characterization ◽  
Data Bank ◽  
Knock Out ◽  
X Ray ◽  
X Ray Crystallography ◽  
Starting Point ◽  
Good Starting Point

Abstract Together with genome analysis and knock-out mutants, structural and functional characterization of proteins provide valuable hints on the biology of the organism under investigation. Structural characterization can be achieved by techniques such as X-ray crystallography, NMR, Cryo-EM. The information derived from the structure are a good starting point to comprehend the details of the proteins molecular function for a better understanding of their biological role. This review aims at describing the progress in the structural and functional characterization of proteins from the plant pathogen Erwinia amylovora obtained by structural biology and currently deposited in the Protein Data Bank.

scholarly journals Characterization of Serracin P, a Phage-Tail-Like Bacteriocin, and Its Activity against Erwinia amylovora, the Fire Blight Pathogen

2002 ◽  
Vol 68 (11) ◽  
pp. 5704-5710 ◽  
Author(s):  
Abdelhamid Jabrane ◽  
Ahmed Sabri ◽  
Philippe Compère ◽  
Philippe Jacques ◽  
Isabel Vandenberghe ◽  
...  
Keyword(s):  
Fire Blight ◽  
Erwinia Amylovora ◽  
Culture Supernatant ◽  
Bacterial Disease ◽  
Amino Acid Sequence Analysis ◽  
Terminal Amino Acid ◽  
Phage Tail ◽  
Terminal Amino ◽  
Pear Trees

ABSTRACT Serratia plymithicum J7 culture supernatant displayed activity against many pathogenic strains of Erwinia amylovora, the causal agent of the most serious bacterial disease of apple and pear trees, fire blight, and against Klebsiella pneumoniae, Serratia liquefaciens, Serratia marcescens, and Pseudomonas fluorescens. This activity increased significantly upon induction with mitomycin C. A phage-tail-like bacteriocin, named serracin P, was purified from an induced culture supernatant of S. plymithicum J7. It was found to be the only compound involved in the antibacterial activity against sensitive strains. The N-terminal amino acid sequence analysis of the two major subunits (23 and 43 kDa) of serracin P revealed high homology with the Fels-2 prophage of Salmonella enterica, the coliphages P2 and 168, the φCTX prophage of Pseudomonas aeruginosa, and a prophage of Yersinia pestis. This strongly suggests a common ancestry for serracin P and these bacteriophages.

scholarly journals Characterization of Myoviridae and Podoviridae family bacteriophages of Erwinia amylovora from Hungary - potential of application in biological control of fire blight

2017 ◽  
Vol 149 (3) ◽  
pp. 639-652 ◽  
Author(s):  
I. Schwarczinger ◽  
J. Kolozsváriné Nagy ◽  
A. Künstler ◽  
L. Szabó ◽  
K. Geider ◽  
...  
Keyword(s):  
Biological Control ◽  
Fire Blight ◽  
Erwinia Amylovora

scholarly journals Testing the virulence of some Hungarian Erwinia amylovora strains on in vitro cultured apple rootstocks

2000 ◽  
Vol 6 (4) ◽  
Author(s):  
M. Hevesi ◽  
J. Papp ◽  
E. Jámbor-Benczúr ◽  
K. Kaszáné Csizmár ◽  
I. Pozsgai ◽  
...  
Keyword(s):  
Host Plants ◽  
Fire Blight ◽  
Erwinia Amylovora ◽  
Disease Development ◽  
Apple Rootstocks ◽  
Disease Rating ◽  
Different Strains

A useful method was improved to test and to evaluate the susceptibility of plants to fire blight and the virulence of E. amylovora strains. Six Hungarian strains from different host plants were tested on in vitro cultured apple rootstocks. Disease rating was used for the characterization of the process of disease development. The different strains had different capacity to cause disease, mainly in the first period of incubation. There were significant differences between the virulence of the strains.

A complete structural characterization of the desferrioxamine E biosynthetic pathway from the fire blight pathogen Erwinia amylovora

2018 ◽  
Vol 202 (3) ◽  
pp. 236-249 ◽  
Author(s):  
Marco Salomone-Stagni ◽  
Joseph D. Bartho ◽  
Ivan Polsinelli ◽  
Dom Bellini ◽  
Martin A. Walsh ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Biosynthetic Pathway ◽  
Fire Blight ◽  
Erwinia Amylovora

scholarly journals Functional characterization of the adenine transporter EaAdeP from the fire blight pathogen Erwinia amylovora and its effect on disease establishment in apples and pears

2020 ◽  
Vol 367 (20) ◽  
Author(s):  
Candace R Alexander ◽  
Regan B Huntley ◽  
Neil P Schultes ◽  
George S Mourad
Keyword(s):  
Fire Blight ◽  
Erwinia Amylovora ◽  
Functional Characterization ◽  
Biochemical Properties ◽  
Apple Fruit ◽  
Infection Process ◽  
Disease Symptoms ◽  
Uptake Studies ◽  
Important Disease

ABSTRACT Erwinia amylovora is the causal agent of fire blight, an economically important disease of apples and pears. As part of the infection process, Er. amylovora propagates on different plant tissues each with distinct nutrient environments. Here, the biochemical properties of the Er. amylovora adenine permease (EaAdeP) are investigated. Heterologous expression of EaAdeP in nucleobase transporter-deficient Escherichia coli strains, coupled with radiolabel uptake studies, revealed that EaAdeP is a high affinity adenine transporter with a Km of 0.43 ± 0.09 μM. Both Es. coli and Er. amylovora carrying extra copies of EaAdeP are sensitive to growth on the toxic analog 8-azaadenine. EaAdeP is expressed during immature pear fruit infection. Immature pear and apple fruit virulence assays reveal that an E. amylovora ΔadeP::Camr mutant is still able to cause disease symptoms, however, with growth at a lower level, indicating that external adenine is utilized in disease establishment.

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