Embryogenic cell lines and germination of shoot from somatic embryos ofPunica granatum‘Ganesh’

2016 ◽  
pp. 11-16 ◽  
Author(s):  
M. Falaki Khalilsaraee ◽  
N.T. Meti
Keyword(s):  
Cell Lines ◽  
Somatic Embryos ◽  
Embryogenic Cell ◽  
Embryogenic Cell Lines

scholarly journals Pinus canariensis plant regeneration through somatic embryogenesis

2020 ◽  
Vol 29 (1) ◽  
pp. eSC05
Author(s):  
Ander Castander-Olarrieta ◽  
Paloma Moncaleán ◽  
Itziar A. Montalbán
Keyword(s):  
Somatic Embryogenesis ◽  
Cell Lines ◽  
Somatic Embryos ◽  
Embryogenic Tissue ◽  
Established Cell Line ◽  
Zygotic Embryos ◽  
Pinus Canariensis ◽  
Embryogenic Cell ◽  
Embryogenic Cell Lines ◽  
Established Cell

Aim of the study: To develop an efficient method to regenerate plants through somatic embryogenesis of an ecologically relevant tree species such as Pinus canariensis.Area of study: The study was conducted in the research laboratories of Neiker-Tecnalia (Arkaute, Spain).Material and methods: Green cones of Pinus canariensis from two collection dates were processed and the resulting immature zygotic embryos were cultured on three basal media. The initiated embryogenic tissues were proliferated testing two subculture frequencies, and the obtained embryogenic cell lines were subjected to maturation. Germination of the produced somatic embryos was conducted and acclimatization was carried out in a greenhouse under controlled conditions.Main results: Actively proliferating embryogenic cell lines were obtained and well-formed somatic embryos that successfully germinated were acclimatized in the greenhouse showing a proper growth.Research highlights: This is the first report on Pinus canariensis somatic embryogenesis, opening the way for a powerful biotechnological tool for both research purposes and massive vegetative propagation of this species.Keywords: acclimatization; Canary Island pine; micropropagation; embryogenic tissue; somatic embryo.Abbreviations used: embryogenic tissue (ET); established cell line (ECL);  somatic embryogenesis (SE); somatic embryos (Se’s).

119. Cryopreservation of embryogenic cell lines of Araucaria angustifolia (Bert.) O. Kuntze

Cryobiology ◽  
2011 ◽  
Vol 63 (3) ◽  
pp. 339 ◽  
Author(s):  
Fernanda Piccolo Pieruzzi ◽  
Andre Luis Wendt dos Santos ◽  
Christina Walters ◽  
Eny Iochevet Segal Floh
Keyword(s):  
Cell Lines ◽  
Araucaria Angustifolia ◽  
Embryogenic Cell ◽  
Embryogenic Cell Lines

Long-term cryopreservation of Greek fir embryogenic cell lines: Recovery, maturation and genetic fidelity

Cryobiology ◽  
2011 ◽  
Vol 63 (1) ◽  
pp. 17-25 ◽  
Author(s):  
Jana Krajňáková ◽  
Suvi Sutela ◽  
Tuija Aronen ◽  
Dušan Gömöry ◽  
Angelo Vianello ◽  
...  
Keyword(s):  
Cell Lines ◽  
Genetic Fidelity ◽  
Embryogenic Cell ◽  
Embryogenic Cell Lines

Somatic polyembryogenesis in embryogenic cell masses of Piceaabies (Norway spruce) and Pinustaeda (loblolly pine) after thawing from liquid nitrogen

1987 ◽  
Vol 17 (9) ◽  
pp. 1130-1134 ◽  
Author(s):  
P. K. Gupta ◽  
D. J. Durzan ◽  
B.J. Finkle
Keyword(s):  
Liquid Nitrogen ◽  
Loblolly Pine ◽  
Somatic Embryos ◽  
Cell Mass ◽  
Embryogenic Cell ◽  
Long Term Storage ◽  
Embryogenic Cell Lines ◽  
Improvement Programs ◽  
Term Storage

We describe a method for the possible cryopreservation of embryogenic callus of Piceaabies and Pinustaeda at −196 °C and the regeneration of somatic embryos from thawed cells of subcultured embryonal–suspensor masses. Piceaabies and Pinustaeda were frozen without cryoprotective agent, in the presence of dimethyl sulfoxide (10%), or in a mixture of polyethylene glycol, glucose, and dimethylsulfoxide (10, 8, and 10% w/v, respectively). Cell masses placed in plastic vials or aluminum envelopes were frozen at 1 °C/min to −30 °C and then immersed for 10 min in liquid nitrogen. Cells were thawed rapidly and placed on modified MS subculture medium. Six to seven somatic embryos per gram of fresh weight were regenerated from each piece of frozen cell mass as compared with 12–13 embryos per gram from unfrozen cells. Post-thaw cell growth was inhibited initially by up to 5 weeks. Inhibition was reversed after the third 10-day subculture. Results suggest that the long-term storage of embryogenic cell lines in liquid nitrogen may be feasible for tree improvement programs in circumstances where testing of progeny may take several years.

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