PALAEOXYLOTOMICAL STUDIES IN THE CENOZOIC PETRIFIED FORESTS OF GREECE. PART TWO – CONIFERS

This paper reports the palaeoxylotomical study of petrified conifer remains from Velitzelos collection, orig-inating from some fossiliferous sites of Greece, especially from the Aegean area (Evros, Limnos, Lesbos), aged of late Oligocene to early Miocene. Ten species were identified: Cupressinoxylon akdikii, Juniperoxylon acarcae, Tetraclinoxylon velitzelosii, Taxodioxylon gypsaceum, Taxodioxylon taxodii, Glyptostroboxylon rudolphii, Glyptostroboxylon tenerum, Pinuxylon pineoides, Pinuxylon halepensoides and Pinuxylon sp. aff. Pinus canariensis. These new identifications add new elements to the forest assemblages of the Oligocene - Miocene Greek flora, useful for understanding the evolution of the Cenozoic palaeovegetation and palaeoclimate in the Aegean area.

Coexistent Heteroblastic Needles of Adult Pinus canariensis C.Sm. ex DC. in Buch Trees Differ Structurally and Physiologically

Great variation in shape and size between primary (juvenile) and secondary (adult) needles, so-called leaf-heteroblasty, occurs in several Pinus species. Most of them loss primary needles during the juvenile-to-adult transition of the tree. An exception to this is Pinus canariensis (a Canary Islands endemism) in which basal resprouting twigs of adult trees frequently wear both primary and secondary needles. Taking advantage of this extraordinary study-case-species, we conducted an exhaustive comparison of both needle types through quantitative analyses of needle anatomy, photochemical performance, gas exchange, and resistance to extreme dehydration and to extreme needle temperature. We hypothesized that primary needles would show lower investment to leaf structure but higher photosynthetical efficiency. Primary needles had less stomatal density and thicker and less wettable cuticles. In cross section, primary needles showed smaller structural fraction (e.g., percent of hypodermis, endodermis and vascular tissue) and higher fraction of photosynthetic parenchyma. Significant differences between primary and secondary needles were not found in net carbon assimilation not in their leaf mass area values. Interestingly, secondary needles showed higher electron transport rate, and they were additionally much more efficient in retaining water under severe and controlled desiccant conditions. When subjected to extreme temperatures (−10° to +50 °C), primary needles recovered better their photochemical efficiency than secondary needles, after +46° and +48 °C heat-shock treatments. Our results indicate that both needle types broaden the diversity of physiological responses against environmental constrains in basal twigs of adult P. canariensis trees. Considering that this is a fire-resistant and resprouting species, this advantage could be particularly useful after a drastic environmental change such a fire or a gap opening in the forest.

Study of the current vegetation of the historical lava flows of the Arafo Volcano, Tenerife, Canary Islands, Spain

Vegetation research on the lava flows of the historic volcanic eruption of 1705 in Arafo, Tenerife, Canary Islands, is presented. The study area located in the 830000-year-old valley of Güímar was created after a massive landslide 47 km3 in volume. The research is divided into three parts, which cover an altitudinal range from around 35 to 1583 m a.s.l. from the Lower-semiarid Inframediterranean up to the Lower-dry lower-Mesomediterranean bioclimatic belts. First, a phytosociological study of the vegetation present in the area was made and concluded that richness in pioneer communities form a vegetation complex with a high degree of endemicity. Two new associations and four pioneer communities are proposed. Especially notable are the communities of Stereocauletum vesuviani and the pioneer communities of Pinus canariensis. The second part of the research was a field sampling study of 450 individuals of Pinus canariensis, which were measured at different altitudes to obtain data about the colonization dynamics of this species on this 300 years old substrate. We found that stem diameter seems to be a good indicator for healthy tree development at a range between 700 to 1300 m asl, which corresponds to the pine forest as potential vegetation and that many individuals show signs of nutrient deficiency. The third part consists of the publication of two new populations of the Canarian endemism Himantoglossum metlesicsianum, a highly endangered orchid. The monitoring of these two populations has recently begun, and further research will be conducted on all three aspects of this publication, which will be presented and expanded upon in the future.

Characterisation of Pinus canariensis C.Sm. ex DC. Sawn Timber from Reforested Trees on the Island of Tenerife, Spain

Canary Island pine (Pinus canariensis C.Sm. ex DC) is a species endemic to the Canary Islands (Spain) that was for centuries overexploited for its wood and resin. Due to the state of the pine forest, more than 10,000 hectares were reforested in the middle of the 20th century. Now, under the silvicultural management plan, thinning operations have allowed Canary Island pine wood to be mechanically characterised for the first time using large test pieces. In total, 1529 pieces measuring 2600 × 120 × 35 mm and visually graded according to Spanish standard UNE 56544 (Visual grading of large structural coniferous sawn timber) were assessed, resulting in 872 pieces in grades ME-1 and ME-2 and 657 rejects. After the characteristic values of density (479–453 kg∙m−3), modulus of elasticity (MOE) (14,023–11,276 N∙mm−2) and bending strength (MOR) (26–14 N∙mm−2) were determined for both grades (ME-1 and ME-2), strength class C24 was assigned to grade ME-1, with similar values to Pinus radiata D.Don and Pinus pinaster Aiton, and C14 was assigned to grade ME-2. Density, number of growth rings, growth ring width, and presence/absence of resinous wood have a significant influence on MOE and MOR, for a confidence level of 95%. Reforestation of Canary Island pine not only allows restoration of the forest cover, but also provides an opportunity, through thinning, to obtain quality wood, helping to create employment and associated industry. This local example with an endemic species can be extrapolated to other parts of the world.

Pinus canariensis plant regeneration through somatic embryogenesis

Aim of the study: To develop an efficient method to regenerate plants through somatic embryogenesis of an ecologically relevant tree species such as Pinus canariensis.Area of study: The study was conducted in the research laboratories of Neiker-Tecnalia (Arkaute, Spain).Material and methods: Green cones of Pinus canariensis from two collection dates were processed and the resulting immature zygotic embryos were cultured on three basal media. The initiated embryogenic tissues were proliferated testing two subculture frequencies, and the obtained embryogenic cell lines were subjected to maturation. Germination of the produced somatic embryos was conducted and acclimatization was carried out in a greenhouse under controlled conditions.Main results: Actively proliferating embryogenic cell lines were obtained and well-formed somatic embryos that successfully germinated were acclimatized in the greenhouse showing a proper growth.Research highlights: This is the first report on Pinus canariensis somatic embryogenesis, opening the way for a powerful biotechnological tool for both research purposes and massive vegetative propagation of this species.Keywords: acclimatization; Canary Island pine; micropropagation; embryogenic tissue; somatic embryo.Abbreviations used: embryogenic tissue (ET); established cell line (ECL); somatic embryogenesis (SE); somatic embryos (Se’s).