scholarly journals The genetic structure of populations of specially protected species Androsace kozo-poljanskii ovsz. in conditions of the south at the central Russian upland using dna markers

2016 ◽  
Vol 14 (1) ◽  
pp. 3
Author(s):  
Edward A Snegin ◽  
Elena A Snegina ◽  
Tatiana A Novomlinskaya
Keyword(s):  
Genetic Structure ◽  
Dna Markers ◽  
The South ◽  
Protected Species ◽  
Genetic Structure Of Populations ◽  
Central Russian Upland

scholarly journals Genetic structure of the water frog (Pelophylax esculentus complex) populations in the south of the Central Russian Upland

2021 ◽  
Vol 19 (2) ◽  
pp. 107-119
Author(s):  
Anatoliy S. Barkhatov ◽  
Eduard A. Snegin ◽  
Sergeu R. Yusupov
Keyword(s):  
Genetic Structure ◽  
Gene Exchange ◽  
Water Frog ◽  
Genetic Structure Of Populations ◽  
Central Russian Upland ◽  
Pcr Products ◽  
Glacial Epoch ◽  
Pelophylax Esculentus ◽  
High Level ◽  
Dna Microsatellite

BACKGROUND: The water frog (Pelophylax esculentus complex) is hybrid in composition. In view of the fact that a large number of data on the species composition of the water frog and very scarce material on the genetic structure of populations are available in the literature, we aimed to analyze the genetic structure of populations of the water frog in the southern part of the Middle Russian upland, which was one of the refugia for many species during the glacial epoch and the center of dispersion in the postglacial time, based on DNA microsatellite markers. MATERIALS AND METHODS: The study involved 36 local populations. DNA variability was analyzed by multiplex SSR-PCR. Seven loci (Res 14, Res 15, Res 17, Res 22, Rrid059A, Rrid082A, and Rrid171A) were used for amplification. Fragment analysis of PCR products was performed on an ABI PRISM 3500 automated capillary DNA sequencer (Applied Biosystems, USA). RESULTS: The total number of alleles detected ranged from 13 to 41. The effective number of alleles (Ae) averaged 4.569 0.219, the Chenon index (I) 1.567 0.04, level of expected heterozygosity (Не) 0.68 0.01. According to Wrights model, the greatest contribution to genetic variability is made by the heterogeneity of individuals within populations, some of which are of a hybrid nature (Fis = 0.281 0.069, Fit = 0.413 0.053, Fst = 0.180 0.017). The average indicator of the intensity of gene exchange between populations (Nm) was 1.212 0.142 individuals per generation. The calculation of the effective abundance using the LD method indicates a high level of viability of the studied groups of the frogs. CONCLUSION: The results demonstrated a high level of genetic diversity and viability of most of the studied groups, which, due to the intense gene exchange between them, can represent a single panmictic population. The data of the genetic analysis support the active adaptation of P. esculentus complex to living in an urbanized environment.

Genetic Relationships of Cory's Shearwater: Parentage, Mating Assortment, and Geographic Differentiation Revealed by DNA Fingerprinting

The Auk ◽  
2000 ◽  
Vol 117 (3) ◽  
pp. 651-662 ◽  
Author(s):  
Corinne Rabouam ◽  
Vincent Bretagnolle ◽  
Yves Bigot ◽  
Georges Periquet
Keyword(s):  
Genetic Variation ◽  
Genetic Structure ◽  
Dna Fingerprinting ◽  
Genetic Relatedness ◽  
Isolation By Distance ◽  
Genetic Relationships ◽  
Cory’S Shearwater ◽  
Genetic Structure Of Populations ◽  
The Social ◽  
Calonectris Diomedea

Abstract We used DNA fingerprinting to assess genetic structure of populations in Cory's Shearwater (Calonectris diomedea). We analyzed mates and parent-offspring relationships, as well as the amount and distribution of genetic variation within and among populations, from the level of subcolony to subspecies. We found no evidence of extrapair fertilization, confirming that the genetic breeding system matches the social system that has been observed in the species. Mates were closely related, and the level of genetic relatedness within populations was within the range usually found in inbred populations. In contrast to previous studies based on allozymes and mtDNA polymorphism, DNA fingerprinting using microsatellites revealed consistent levels of genetic differentiation among populations. However, analyzing the two subspecies separately revealed that the pattern of genetic variation among populations did not support the model of isolation by distance. Natal dispersal, as well as historic and/or demographic events, probably contributed to shape the genetic structure of populations in the species.

Sign in / Sign up

Export Citation Format

Share Document