scholarly journals Determination of the concentration of circulating immune complexes, immunoglobulin E and G and histamine in the blood and lymph in experimental anaphylactic shock and Arthus phenomenon

2018 ◽  
Vol 99 (1) ◽  
pp. 59-63
Author(s):  
T R Alieva
Keyword(s):  
Immune Complexes ◽  
Anaphylactic Shock ◽  
Immunoglobulin E ◽  
Circulating Immune Complexes ◽  
Arthus Phenomenon

Цель. Изучение изменения содержания концентрации циркулирующих иммунных комплексов, уровней иммуноглобулинов E и G и гистамина в крови и лимфе при экспериментальном анафилактическом шоке и феномене Артюса. Методы. Эксперименты проведены на 27 кроликах породы шиншилла в двух сериях. Контролем служили исследуемые показатели концентраций циркулирующих иммунных комплексов, гистамина и иммуноглобулинов E и G в крови и лимфе интактных животных. Результаты. Уровень гистамина при анафилактическом шоке повышается в 3,8 раза, тогда как уровень иммуноглобулинов E и G повышается соответственно в 14,7 и 3,5 раза в сравнении с интактными животными, а концентрация циркулирующих иммунных комплексов возрастает до 23 ммол/л за этот период. В условиях феномена Артюса уровень иммуноглобулина E в крови повышается в 1,3 раза, а иммуноглобулина G - в 4,3 раза. Концентрация циркулирующих иммунных комплексов возрастает в 11,8 раза, уровень гистамина повышается только в 3,5 раза в сравнении с интактными животными. Вывод. Концентрация циркулирующих иммунных комплексов повышается как при анафилактическом шоке, так и при феномене Артюса; уровни иммуноглобулинов изменяются по-разному: при анафилактическом шоке происходит увеличение уровня иммуноглобулина E и снижение иммуноглобулина G, а при феномене Артюса, наоборот, повышение уровня иммуноглобулина G и снижение иммуноглобулина E; уровень гистамина увеличивается как при анафилактическом шоке, так и при феномене Артюса, однако при анафилактическом шоке - более значимо; в повышении концентрации гистамина в обеих аллергических реакциях роль циркулирующих иммунных комплексов не исключена, но основная роль, по-видимому, принадлежит иммуноглобулину E.

scholarly journals Comparative measurement of concentration of lithium, circulating immune complexes and complement activity in blood and lymph in experimental anaphylactic shock and Arthus phenomenon

2018 ◽  
Vol 99 (4) ◽  
pp. 635-639
Author(s):  
T R Alieva
Keyword(s):  
Immune Complexes ◽  
Blood Concentration ◽  
Anaphylactic Shock ◽  
Lithium Concentration ◽  
Sharp Decrease ◽  
Circulating Immune Complexes ◽  
Comparative Measurement ◽  
Complement Components ◽  
Complement Activity ◽  
Arthus Phenomenon

Aim. Comparative measurement of concentration of lithium, circulating immune complexes and complement activity in animals’ blood and lymph in experimental anaphylactic shock and Arthus phenomenon. Methods. Experiments were conducted on 27 chinchilla rabbits in double series. The studied values of lithium, concentration of circulating immune complexes and complement activity in intact animals were used as controls. Results. On day 7 of sensibilisation before anaphylactic shock the titer of complement components in the blood decreased by 1.4 times (p <0.001) compared to parameters of intact animals, at the stage of anaphylactic shock sharp decrease of its concentration to undetermined values was observed (p <0.001). The blood concentration of circulating immune complexes increased by 4.4 times, and at the stage of anaphylactic shock (day 21) its increase by 7.1 times was observed (p <0.001). Lithium concentration decreased to 0.03 mmol/L at the stage of anaphylactic shock (p <0.001). In Arthus phenomenon at the stage of sensibilisation (day 5) the blood complement titer decreased by 1.1 times (p <0.001), during resolution period - by 10.1 times (p <0.001). The blood concentration of circulating immune complexes increased by 4.5 times (p <0.001) at the stage of sensibilisation (day 5), and at the stage of Arthus phenomenon - by 11.8 times compared to intact animals (p <0.001). Conclusion. The concentration of circulating immune complexes increases in anaphylactic and immunocomplex reactions, besides, more significantly in Arthus phenomenon (23 r.u. in anaphylactic shock versus 38.1 r.u. in Arthus phenomenon); lithium concentration in anaphylactic shock decreases by 5.2 times which is indicative of inhibitory action of this microelement in allergic reactions.

PARTICULARITIES OF THE IMMUNE RESPONSE IN PERSONS PROFESSIONNALLY EXPOSED TO BERYLLIUM

2016 ◽  
pp. 26-30
Author(s):  
K. I. Stosman ◽  
L. V. Lukovnikova
Keyword(s):  
Immune Response ◽  
Immune Complexes ◽  
Immunoglobulin E ◽  
Circulating Immune Complexes ◽  
Professional Contact ◽  
Beryllium Compounds

An examination was performed of 50 employees at an enterprise where they were in professional contact with beryllium. In most workers, it was detected an increase of interleukine-8, interferon- , growing level of immunoglobulin E and circulating immune complexes. It was shown that the contact with beryllium compounds leads to the interferon- level growth only in women. In men, alterations are identified in the direction of increased concentrations of common immunoglobulin E and circulating immune complexes.

scholarly journals Influence of Melatonin on the state of immunity at psoriasis

2009 ◽  
Vol 8 (4) ◽  
pp. 148-151
Author(s):  
V. S. Dmitruk ◽  
L. V. Striga
Keyword(s):  
Immune System ◽  
Polyethylene Glycol ◽  
Immune Complexes ◽  
Combined Treatment ◽  
Circulating Immune Complexes ◽  
Selective Precipitation ◽  
Exogenous Melatonin ◽  
Immunomodulatory Action ◽  
Immunofluorescent Method

The influence of the chronopharmacological scheme of treatment with the use of exogenous Melatonin in the form of the Melaksen drug on the immunity status of psoriasis patients has been studied. The study of the immunity status consisted in determination of the main Manchini classes of immunoglobulins A, M, G, the level of circulating immune complexes by the method of selective precipitation in 3% polyethylene glycol. In addition, we determined the subpopulation of lymphocytes carrying CD3, CD4, CD8, CD16, CD 25, and CD72 antigens by the immunofluorescent method. In has been found that Melatonin in psoriasis patients exerts the immunomodulatory action on the immune system at the combined treatment (due to an increase in the number of -lymphocytes with cytotoxic-suppressive properties at obvious signs of immune disbalance in the form of the increased ratio of Т CD4+ and CD8+).

A method for quantitative determination of average masses and concentrations of circulating immune complexes in human sera

1983 ◽  
Vol 6 (1) ◽  
pp. 29-32 ◽  
Author(s):  
K.K. Ostreiko ◽  
I.A. Tumanova ◽  
Ju.K. Sykulev ◽  
V.V. Lavrentiev
Keyword(s):  
Quantitative Determination ◽  
Immune Complexes ◽  
Circulating Immune Complexes ◽  
Human Sera

scholarly journals ОСОБЛИВОСТІ ІМУННОГО ГОМЕОСТАЗУ В ДИНАМІЦІ РОЗВИТКУ ЕКСПЕРИМЕНТАЛЬНОЇ БРОНХІАЛЬНОЇ АСТМИ

2018 ◽  
Author(s):  
M. S. Reheda ◽  
L. A. Lubinets ◽  
B. F. Shchepanskyi
Keyword(s):  
Immune System ◽  
B Lymphocytes ◽  
Immune Complexes ◽  
Guinea Pigs ◽  
Circulating Immune Complexes ◽  
System Response ◽  
Control Group ◽  
Modeling Process ◽  
Immune System Response

In this paper, it is shown that the modeling process of bronchial asthma (BA ) is accompanied by changes, in comparison with the control group, in the indicators of immune system response: T- & B-lymphocytes, circulating immune complexes in blood of guinea pig males on the 4th, 18th, 25th day of experiment.The aim of the study – determination of some indices of immune system in blood of guinea pigs in the modeling process of BA on 4th, 18th, 25th day of the experiment.Materials and Methods. Experiments were conducted on 40 guinea pigs (males), with body weight 0.25–0.27 kg. Animals were divided into four groups of ten animals in each. Intact guinea pigs were the first group. Animals with experimental BA – the second, third, fourth group respectively on the 4th, 18th, 25th day of the experiment. Experimental BA was reproduced by V. I. Babych method. In blood of intact guinea pigs and animals with experimental BA , the number of T- and B-lymphocytes was determined by the method of E. F. Chernushenko, L. S. Kohosov, determination of the level of circulating immune complexes was carried out by the method of V. Haskova and co-authors. The results of the study were processed by the method of variation statistics using Student's criterion.Results and Discussion. The results of the studies showed unidirectional changes in individual parameters of the immune system, depending on the periods of the formation of BA : an increase in the number of B-lymphocytes and circulating immune complexes, a decrease in the number of T-lymphocytes for all of the studied days of the experiment.Conclusions. The obtained results indicate significant changes in the immune system parameters in the blood of experimental animals with BA and are important for understanding the pathogenesis of BA . These studies provide an opportunity to find the more perfect and effective methods of diagnosis of BA.

scholarly journals Method for the determination of immune complexes by reaction with polyethylene glycol

1982 ◽  
Vol 63 (2) ◽  
pp. 10-13
Author(s):  
B. A. Molotilov ◽  
A. N. Mayansky ◽  
N. D. Pozdnyak ◽  
L. Ch. Samerkhanova
Keyword(s):  
Polyethylene Glycol ◽  
Lupus Erythematosus ◽  
Immune Complexes ◽  
Clinical Laboratory ◽  
Circulating Immune Complexes ◽  
Healthy Donors ◽  
Highly Sensitive ◽  
Photoelectric Colorimeter ◽  
Health And Disease

A study of circulating immune complexes was carried out using a reaction with polyethylene glycol. The method turned out to be simple, highly sensitive and affordable for any clinical laboratory with a photoelectric colorimeter. Analysis of the survey data of 115 healthy donors, 63 patients with rheumatoid arthritis and 16 patients with systemic lupus erythematosus made it possible to establish the level of circulating immune complexes in health and disease. The circulating immune complexes were studied in patients with rheumatism and chronic tonsillitis. To assess the results of the reaction, human aggregated gamma globulin (manufactured by Kazan NIIEM) was used.

Determination of the half-life of C3 in patients and its relation to the presence of C3-breakdown products and/or circulating immune complexes

1982 ◽  
Vol 2 (4) ◽  
pp. 161-166 ◽  
Author(s):  
A. J. G. Swaak ◽  
A. Hannema ◽  
C. Vogelaar ◽  
F. A. Boom ◽  
L. van Es ◽  
...  
Keyword(s):  
Immune Complexes ◽  
Half Life ◽  
Circulating Immune Complexes

Determination of circulating immune complexes by chicken anti-human C3 and anti-human C1q microELISA

1988 ◽  
Vol 113 (1) ◽  
pp. 93-99 ◽  
Author(s):  
Anders Larsson ◽  
Lars Jonsson ◽  
John Sjöquist
Keyword(s):  
Immune Complexes ◽  
Circulating Immune Complexes

scholarly journals The Importance of The Role of Circulating Immune Complexes in Determination of Clinical Activity in Systemic Lupus Erythematosus

1996 ◽  
Vol 6 (1) ◽  
pp. 75-79
Author(s):  
Ali Kokuludağ ◽  
Ender Terzioğlu ◽  
Aytül Sin ◽  
İbrahim Kıyıcı ◽  
Şükran Köse ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Immune Complexes ◽  
Circulating Immune Complexes ◽  
Clinical Activity ◽  
Systemic Lupus
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