scholarly journals ОСОБЛИВОСТІ ІМУННОГО ГОМЕОСТАЗУ В ДИНАМІЦІ РОЗВИТКУ ЕКСПЕРИМЕНТАЛЬНОЇ БРОНХІАЛЬНОЇ АСТМИ

2018 ◽  
Author(s):  
M. S. Reheda ◽  
L. A. Lubinets ◽  
B. F. Shchepanskyi
Keyword(s):  
Immune System ◽  
B Lymphocytes ◽  
Immune Complexes ◽  
Guinea Pigs ◽  
Circulating Immune Complexes ◽  
System Response ◽  
Control Group ◽  
Modeling Process ◽  
Immune System Response

In this paper, it is shown that the modeling process of bronchial asthma (BA ) is accompanied by changes, in comparison with the control group, in the indicators of immune system response: T- & B-lymphocytes, circulating immune complexes in blood of guinea pig males on the 4th, 18th, 25th day of experiment.The aim of the study – determination of some indices of immune system in blood of guinea pigs in the modeling process of BA on 4th, 18th, 25th day of the experiment.Materials and Methods. Experiments were conducted on 40 guinea pigs (males), with body weight 0.25–0.27 kg. Animals were divided into four groups of ten animals in each. Intact guinea pigs were the first group. Animals with experimental BA – the second, third, fourth group respectively on the 4th, 18th, 25th day of the experiment. Experimental BA was reproduced by V. I. Babych method. In blood of intact guinea pigs and animals with experimental BA , the number of T- and B-lymphocytes was determined by the method of E. F. Chernushenko, L. S. Kohosov, determination of the level of circulating immune complexes was carried out by the method of V. Haskova and co-authors. The results of the study were processed by the method of variation statistics using Student's criterion.Results and Discussion. The results of the studies showed unidirectional changes in individual parameters of the immune system, depending on the periods of the formation of BA : an increase in the number of B-lymphocytes and circulating immune complexes, a decrease in the number of T-lymphocytes for all of the studied days of the experiment.Conclusions. The obtained results indicate significant changes in the immune system parameters in the blood of experimental animals with BA and are important for understanding the pathogenesis of BA . These studies provide an opportunity to find the more perfect and effective methods of diagnosis of BA.

scholarly journals Influence of Melatonin on the state of immunity at psoriasis

2009 ◽  
Vol 8 (4) ◽  
pp. 148-151
Author(s):  
V. S. Dmitruk ◽  
L. V. Striga
Keyword(s):  
Immune System ◽  
Polyethylene Glycol ◽  
Immune Complexes ◽  
Combined Treatment ◽  
Circulating Immune Complexes ◽  
Selective Precipitation ◽  
Exogenous Melatonin ◽  
Immunomodulatory Action ◽  
Immunofluorescent Method

The influence of the chronopharmacological scheme of treatment with the use of exogenous Melatonin in the form of the Melaksen drug on the immunity status of psoriasis patients has been studied. The study of the immunity status consisted in determination of the main Manchini classes of immunoglobulins A, M, G, the level of circulating immune complexes by the method of selective precipitation in 3% polyethylene glycol. In addition, we determined the subpopulation of lymphocytes carrying CD3, CD4, CD8, CD16, CD 25, and CD72 antigens by the immunofluorescent method. In has been found that Melatonin in psoriasis patients exerts the immunomodulatory action on the immune system at the combined treatment (due to an increase in the number of -lymphocytes with cytotoxic-suppressive properties at obvious signs of immune disbalance in the form of the increased ratio of Т CD4+ and CD8+).

scholarly journals The influence of thiotriazoline on the change of immune system in the blood of guinea-pigs under the conditions of development of experimental bronchial asthma

2015 ◽  
Vol 17 (2) ◽  
Author(s):  
M. S. Reheda ◽  
М. А. Kolishetska ◽  
V. R. Yurevych
Keyword(s):  
Immune System ◽  
T Lymphocytes ◽  
Blood Plasma ◽  
B Lymphocytes ◽  
Bronchial Asthma ◽  
Functional State ◽  
Immune Complexes ◽  
Guinea Pigs ◽  
Stimulation Of ◽  
Plasma Activity

<p>The aim of our research was to determine the character of the role and functional state of separate indexes<br />of the immune system in blood of guinea-pigs under the conditions of the development of experimental bronchial<br />asthma (BA ) and estimation of thiotriazoline influence on them. Decreasing of T-lymphocytes, stimulation of humoral<br />link of immunity, namely increasing of B-lymphocytes and immunoglobulins of A, M and G, elevation of circulatory<br />immune complexes and slump of complement blood plasma activity had been determined in this research. Immune<br />correcting action of thiotriazoline upon the pointed out indices in case of BA is revealed.</p>

scholarly journals Clinical and immunological features of opisthorchiasis

2021 ◽  
Vol 102 (5) ◽  
pp. 626-635
Author(s):  
A U Sabitov ◽  
D A Soldatov ◽  
Yu B Khamanova
Keyword(s):  
Immune System ◽  
Immune Complexes ◽  
Statistical Significance ◽  
Rank Correlation ◽  
Statistical Processing ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
Cytotoxic Lymphocytes ◽  
Spearman’S Rank Correlation ◽  
Diagnostic Center

Aim. To assess the clinical and immunological features in patients with chronic opisthorchiasis, depending on the duration of the infection. Methods. The first group consisted of 19 patients with the duration of the infection up to 1 year, the second group consisted of 21 patients with the duration of the infection between 1 and 5 years, the third group was formed of 23 patients with the duration of the disease more than 5 years, the control group 20 healthy individuals. Immunological research was carried out at the Clinical Diagnostic Center. Statistical processing was performed using Microsoft Excel 2010 and Statistica 6.0 software. The statistical significance of differences was determined by using the MannWhitney test (U-test) at the level of significance of p 0.05. The correlations were assessed by calculating Spearman's rank correlation coefficients.Results Clinical features of chronic opisthorchiasis were revealed in the disease duration groups of up to 1 year, from 1 year to 5 years, more than 5 years: the subclinical course was most common in the group of up to 1 year; cholangiohepatitis prevailed in the group of between 1 to 5 years, allergic skin syndrome, cholangiocholecystitis and pancreatitis dominated in the group of more than 5 years. The immune response in chronic opisthorchiasis was characterized by: up to 1 year lymphocytosis, increased levels of immunoglobulins M (IgM) and circulating immune complexes (CIC), a decrease in the number of T-lymphocytes (CD3+), as well as an increase in bactericidal activity of leukocytes (BAL); between 1 and 5 years monocytosis, increased levels of immunoglobulins M, immunoglobulins G and circulating immune complexes, a decrease in T-cytotoxic lymphocytes (CD8+) and nitro blue tetrazolium (NBT test), as well as an increase in NK cells and phagocytic activity of monocytes, more than 5 years eosinophilia. Conclusion. Common features of rearrangement of the immune system in opisthorchiasis: inflammatory changes in the hemogram, activation of humoral immunity with parallel suppression of the cellular component of the immune system, and increased phagocytosis.

scholarly journals Down-Expression of miRNA-98 and Over-Expression of miRNA-9 Can Result in Inadequate Immune System Response against Lung Cancer

2021 ◽  
Vol 11 (6) ◽  
pp. 13893-13902
Keyword(s):  
Lung Cancer ◽  
Immune System ◽  
Real Time ◽  
Real Time Pcr ◽  
Cytokine Level ◽  
System Response ◽  
Control Group ◽  
Incidence And Mortality ◽  
Immune System Response ◽  
Nsclc Patients

Lung cancer is a disease presenting high incidence and mortality rates. Its most common type is non-small cell lung cancer (NSCLC), constituting about 80-85% of all cases. MicroRNAs (miRNAs) are the molecules playing a role in the regulation of genes. Two crucial cytokines in cancer are interleukin (IL)-10 and IFN-γ. Our objective was to investigate the expression of miRNA-9, miRNA-98, JAK, and STAT3 in patients with NSCLC and detect the cytokine level of IL-10. A total of 50 patients with NSCLC participated in this study. Besides, 50 healthy blood samples were selected as the control group. Real-time PCR determined the expression levels of miRNAs so that the RNAs extracted from the patients' peripheral mononuclear cells (PBMC) were initially synthesized, and cDNA was then extracted. Finally, the synthesized cDNA was amplified using real-time PCR, and its expression was compared to the control group. ELISA technique was used to detect IL-10 in plasma. Our result showed a low level of expression of miRNA-98, JAK, and STAT3 and a high-level expression of miRNA-9. ELISA test indicated a high cytokine level of IL-10 in the NSCLC patients' serum compared to the healthy controls. MiRNA-9 could suppress JAK and STAT3 genes in the NSCLC patients, which causes the poor immune response against the cell's proliferation. Besides, IL-10 was enhanced when miRNA-98 was reduced. Therefore, the elevation of IL-10, as an anti-inflammatory cytokine, could lead to lung cancer progression without a strong immune system response.

The systemic immunity humoral link status in patients with traumatic illness

2014 ◽  
Vol 18 (4 (72)) ◽  
Author(s):  
I. M. Plehutsa ◽  
R. I. Sydorchuk
Keyword(s):  
Immune System ◽  
B Lymphocytes ◽  
Cellular Immunity ◽  
Surgical Intervention ◽  
Traumatic Injury ◽  
Control Group ◽  
Immune Disorders ◽  
Comparison Groups ◽  
Integrated Indicators

The influence of trauma on parameters of humoral immunity changes has been studied. The study includes 52 patients with various forms of traumatic illness, aged 18-69 years (37,91±4,28). The control group consisted of 16 patients who had undergone a routine surgery not related to the pathology of musculoskeletal system. All patients of the main group were divided into 3 groups according to the severity of their condition. Analysis of parameters of cellular link of immune system was performed by defining the pool of B-lymphocytes (CD22+ lymphocytes), determination of main classes' immunoglobulins, and calculation of integrated indicators. The highest expression (immune disorders of II-III grades) of changes of cellular immunity was observed in patients with severe traumatic illness (detailed clinical picture). Surgical intervention, even without traumatic injury significantly affect cellular immunity, but in patients with traumatic illness immunity disorders were significantly higher than in the comparison groups. 

scholarly journals Порушення імунного гомеостазу в ранній період розвитку виразкової хвороби шунка на тлі експериментальної пневмонії

2017 ◽  
Author(s):  
L. O. Furdychko
Keyword(s):  
Gastric Ulcer ◽  
Immune System ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Immune Complexes ◽  
Experimental Studies ◽  
Early Period ◽  
Circulating Immune Complexes ◽  
Immune Homeostasis ◽  
Experimental Pneumonia

Introduction. The article adduces the obtained results of the indices of immune homeostasis in the development of combined pathology (experimental pneumonia (EP) and gastric ulcer (GU) of the stomach). The immune system is characterized by the number of T-lymphocytes since they are the basis of the cellular component of the immuneresponse and the concentration of b-lymphocytes and circulating immune complexes (CIC), which are responsible for cellular immunity.The aim of the study – the content of T-lymphocytes, CEC to assess the immune system in the development of EP and GU.Materials and Methods. This experimental study was conducted on 39 guinea pigs-males. Experimental pneumonia caused by the method of V. N. Shliapnikova, T. L. Solodova [et al.] Gastric ulcer of the stomach wassimulated by the method of V. I. Komarova. We determined the content of T- and B-lymphocytes (SD3 and SD19) in the blood by the method of E. F. Chernushenko, L. S. Kohosova. The level of circulating immune complexes (CIC) in the blood by the method of Haskova V., Kaslik J..Results and Discussion. The results of experimental studies established that early period of development (4th and 8th day) of gastric ulcer and pneumonia is characterized by the following changes of immune homeostasis: decrease in the level of T-lymphocytes and elevated levels of lymphocytes and circulating immune complexes in the blood of Guinea pigs.Conclusion. A depression of cell growth and humoral types of immunity in terms of experimental pneumoniaand gastric ulcer was revealed.  

scholarly journals The influence of the Selenium and Germanium on humoral immunity content in quails

2020 ◽  
pp. 88-95
Author(s):  
A. Yemelyanenko ◽  
S. Shmayun ◽  
M. Nishmenenko ◽  
O. Yemelyanenko ◽  
O. Poroshinska ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Immune System ◽  
Key Words ◽  
Humoral Immunity ◽  
Immune Complexes ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
Low Molecular Weight ◽  
Main Indicator ◽  
Ig G

The results of the studies on the infl uence of Selenium and Germanium nanoacqualates complex on the content of immunoglobulins and circulating immune complexes in quail’s are presented in the article. It was found that the level of immunoglobulins is the main indicator of the state of humoral immunity. According to the results of the studies the content of immunoglobulins A, M, G in the quail’s serum was 0.80 ± 0.03 mg/ml, 0.30 ± 0.01 mg/ml, and 5.82 ± 0 , 06 mg/ml, accordingly. That are 31.1%, 20.0% and 6.0%, respectively, comparing with data of the control group. Inthe serum of fi ve days old quail’s Ig A content was 0.84 ± 0.02 mg/ml, Ig M 0.54 ± 0.03 mg/ml and Ig G 6.87 ± 0.04 mg/ml, that consisted 29.2%, 42.1% and 5.0%, respectively, comparing with the control group. The content of circulating immune complexes (CEC) in theblood serum of one- and fi ve-days old quails was 1.5 and 1.6 times, respectively lower than in the birds of the control group. The content of low molecular weight CECs in the serum of one- and fi ve-days old quail, decreased signifi cantly in both groups(in 2 and 1.1 times, respectively). This probably characterizes the overall effi cacy and balance of the immune system's responses in quails exposed to Selenium and Germanium nanoacqualates at used doses of 0.05 μg/kg and 5.0 μg/kg, respectively. We consider that as a positive eff ect on the immunity of quails in the early post-embryonic period. Therefore, the use of Selenium and Germanium nanoacqualates complex has a complementary eff ect and helps to increase the immunoreactivity of young animals by maintaining the activity of the immune system. Key words: Selenium nanoacquachelate, Germanium nanoacquachelate, quails, immunoglobulins, immunity.

scholarly journals NCMP-05. DECODING THE IMMUNE SYSTEM RESPONSE TO LEPTOMENINGEAL METASTASIS

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii124-ii124
Author(s):  
Jan Remsik ◽  
Xinran Tong ◽  
Ugur Sener ◽  
Danille Isakov ◽  
Yudan Chi ◽  
...  
Keyword(s):  
Immune System ◽  
Immune Cells ◽  
Leptomeningeal Metastasis ◽  
System Response ◽  
Idle State ◽  
The Central Nervous System ◽  
Health And Disease ◽  
Immune System Response ◽  
Therapeutic Protocols ◽  
Brain And Spinal Cord

Abstract For decades, the central nervous system was considered to be an immune privileged organ with limited access to systemic immunity. However, the leptomeninges, the cerebrospinal fluid (CSF)-filled anatomical structure that protects the brain and spinal cord, represent a relatively immune-rich environment. Despite the presence of immune cells, complications in the CSF, such as infectious meningitis and a neurological development of cancer known as leptomeningeal metastasis, are difficult to treat and are frequently fatal. We show that immune cells entering the CSF are held in an ‘idle’ state that limits their cytotoxic arsenal and antigen presentation machinery. To understand this underappreciated neuroanatomic niche, we used unique mouse models and rare patient samples to characterize its cellular composition and critical signaling events in health and disease at a single-cell resolution. Revealing the mediators of CSF immune response will allow us to re-evaluate current therapeutic protocols and employ rational combinations with immunotherapies, therefore turning the patient’s own immune system into an active weapon against pathogens and cancer.

scholarly journals Cytokine Patterns in Brain Tumour Progression

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Radu Albulescu ◽  
Elena Codrici ◽  
Ionela Daniela Popescu ◽  
Simona Mihai ◽  
Laura Georgiana Necula ◽  
...  
Keyword(s):  
Immune System ◽  
Inflammatory Cytokines ◽  
Tumour Growth ◽  
Tumour Progression ◽  
Inflammatory Cells ◽  
Serum Levels ◽  
Angiogenic Factors ◽  
System Response ◽  
Gm Csf ◽  
Immune System Response

Inflammation represents the immune system response to external or internal aggressors such as injury or infection in certain tissues. The body’s response to cancer has many parallels with inflammation and repair; the inflammatory cells and cytokines present in tumours are more likely to contribute to tumour growth, progression, and immunosuppression, rather than in building an effective antitumour defence. Using new proteomic technology, we have investigated serum profile of pro- (IL-1β, IL-6, IL-8, IL-12, GM-CSF, and TNF-α) and anti-inflammatory cytokines (IL-4, IL-10), along with angiogenic factors (VEGF, bFGF) in order to assess tumoural aggressiveness. Our results indicate significant dysregulation in serum levels of cytokines and angiogenic factors, with over threefold upregulation of IL-6, IL-1β, TNF-α, and IL-10 and up to twofold upregulation of VEGF, FGF-2, IL-8, IL-2, and GM-CSF. These molecules are involved in tumour progression and aggressiveness, and are also involved in a generation of disease associated pain.

scholarly journals Scaling in the Immune System: PhD Thesis

2019 ◽  
Author(s):  
soumya banerjee
Keyword(s):  
Immune Response ◽  
Immune System ◽  
Body Size ◽  
Human Health ◽  
Response Rates ◽  
System Response ◽  
Metabolic Rates ◽  
Viral Dynamics ◽  
Immune System Response ◽  
Phd Thesis

How different is the immune system in a human from that of a mouse? Do pathogens replicate at the same rate in different species? Answers to these questions have impact on human health since multi-host pathogens that jump from animals to humans affect millions worldwide.It is not known how rates of immune response and viral dynamics vary from species to species and how they depend on species body size. Metabolic scalingtheory predicts that intracellular processes will be slower in larger animals since cellular metabolic rates are slower. We test how rates of pathogenesis and immune system response rates depend on species body size.

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