IN VITRO RESPONSE BY Terminalia arjuna GENOTYPES DURING MICROPROPAGATION

2021 ◽  
Vol 9 (1) ◽  
pp. 44-50
Author(s):  
Meena Choudhary ◽  
◽  
Ashok Gehlot ◽  
Sarita Arya ◽  
Inder Dev Arya ◽  
...  
Keyword(s):  
Shoot Proliferation ◽  
In Vitro Rooting ◽  
Terminalia Arjuna ◽  
Ms Medium ◽  
Demand And Supply ◽  
Half Strength ◽  
Modified Ms Medium ◽  
In Vitro Response ◽  
Different Doses

Terminalia arjuna is an important tree of the medicinal and sericulture industry, commonly known as Arjun. It’s bark is rich in secondary metabolites makes this plant highly valuable in medicine industry to treat cardiovascular disease. Overexploitation due to high demand in medicine, low seed germination, limitations of the conventional method of propagation push this plant towards being endangered. To conserve germplasm of such tree species and meet the requirement in medicinal industry, some non-conventional propagation method like micropropagation has been developed. The present work highlighted the effect of three genotypes (G-1, G-2, and G-3) on tissue culture of T. arjuna situated at Jodhpur, Rajasthan, India. In vitro shoot proliferation was achieved on a modified MS medium enriched with BAP + additives. Among the tested genotypes, Genotype -1 showed maximum bud break response (100%) followed by G-3 (93.33 %) and G-2 (86.66%). Further multiplication of these shoots on modified MS medium containing BAP + NAA + additives gave 11.38±0.26 (G-1), 9.44±0.21 (G-2) and 10.22±0.32 (G-3) shoots. In vitro rooting was done by pulse treatment with IBA for 10 min prior to transfer on hormone free half strength MS medium containing 0.1% activated charcoal. Maximum in vitro rooting was obtained in G-1 (80%) followed by G-3 (71.11%) and G-2 (68.88%). In the present study, it was observed that optimum growth in all three genotypes required different doses of Plant Growth Regulator. Thus, by identifying and multiplying the best performing genotypes the gap between demand and supply of such medicinal plant can be fulfilled.

scholarly journals Micropropagation of Eucalyptus Species

HortScience ◽  
1991 ◽  
Vol 26 (2) ◽  
pp. 199-200 ◽  
Author(s):  
J.J. Le Roux ◽  
J. Van Staden
Keyword(s):  
Acetic Acid ◽  
Shoot Growth ◽  
Shoot Proliferation ◽  
Eucalyptus Species ◽  
Ms Medium ◽  
Root Initiation ◽  
Cold Tolerant ◽  
Half Strength ◽  
Modified Ms Medium

Two cold-tolerant species (Eucalyptus macarthurii Deane et Maiden and E. smithii R.T. Baker), a cold-tolerant hybrid (E. macarthurii×E. grandis Hill ex Maiden), and E. saligna Sm. were propagated in vitro from nodal explants collected from field-grown seedlings and from clonal hedges. Shoot growth was initiated on modified Murashige and Skoog (MS) medium containing BA at 0.1 mg·liter-1. Modified MS medium with BA (0.2 mg·liter-1) and NAA (0.01 mg·liter-1) was most effective in promoting shoot proliferation. Root initiation was achieved on half-strength modified MS medium with 2 mg IBA/liter. Rooted plants were hardened and established in the field. Chemical names used: N-(phenylmethyl)-1EZ-purin-6-amine (BA); 2-(1-naphthyl)acetic acid (NAA); 1H-indole-3-butyric acid (IBA).

scholarly journals Effect of Sucrose Concentrations and Incubation Periods on in Vitro Rooting of Moris Pineapple (Ananas comosus)

2019 ◽  
Vol 34 (4) ◽  
pp. 230-242
Author(s):  
Abdelhamid M Hamad
Keyword(s):  
Incubation Period ◽  
In Vitro Rooting ◽  
Ananas Comosus ◽  
Ms Medium ◽  
Incubation Periods ◽  
Half Strength

The effect of 6 sucrose concentrations (5, 10, 15, 20, 25, 30 g/l) over 4 incubation periods (30, 45, 60, 75 days) on in vitro rooting of Moris pineapple cultured in liquid half strength MS medium enriched with IBA at 2.0 mg/l was investigated. At all incubation periods, all shoots in medium enriched with sucrose at 5 g/l failed to root, and no roots formed within the first 30 days in medium enriched with sucrose at 10 g/l. After 30 days of incubation, the highest rooting percentage (66 %), tallest plantlets (23 mm tall), highest (3.4 roots) and longest (5.3 mm) root per shoot were obtained in medium enriched with sucrose at 25, 10, 15, 15 g/l respectively, while after 45 days, the highest of all rooting aspects (75 %, 32.3 mm tall, 3.7 roots, 7 mm long), were obtained in medium enriched with sucrose at 15 g/l. After 60 days, the highest rooting percentage (91.7 %) and tallest plantlets (36.7 mm tall) were obtained in medium enriched with sucrose at 20 g/l while highest roots per shoot (3.7 roots) and longest root (10.7 mm) were obtained in medium enriched with sucrose at 15 g/l. After 75 days, all shoots rooted (100 %) in medium enriched with sucrose at 10 and 20 g/l, while sucrose at 25 g/l resulted in tallest plantlets (46.3 mm tall) and at 20 g/l resulted in highest (4.7 roots) and longest roots (27.3 mm). At each incubation period, there was a different optimum sucrose enrichment for different rooting parameters.

scholarly journals Micropropagation of Achillea millefolium L. on half-strength ms medium and direct rooting and acclimatization of microshoots in hydroponic culture

2015 ◽  
pp. 99-112
Author(s):  
Marija Markovic ◽  
Dragana Skocajic ◽  
Mihailo Grbic ◽  
Matilda Djukic ◽  
Dragica Obratov-Petkovic ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Nutrient Solution ◽  
Ph Value ◽  
Ex Vitro Rooting ◽  
In Vitro Rooting ◽  
Efficient Production ◽  
Ms Medium ◽  
Ex Vitro ◽  
Half Strength

The aim of this study was to determine the possibility of micropropagation of the medicinal plant A. millefolium on half-strength MS medium and ex vitro rooting and acclimatization of the obtained microshoots in hydroculture in order to establish an efficient production method. Two explant types were used: basal and terminal cuttings, and better results were achieved when terminal cuttings were used. The development of shoots in the multiplication phase was successful with a regeneration percentage of 100%. Ex vitro rooting in a modified Hoagland nutrient solution was successful (83%), but the percentage of in vitro rooting on half-strength MS medium without hormones was higher (95%). However, bearing in mind that mass production of A. millefolium is more efficient when the phase of in vitro rooting is excluded, this method could be recommended for commercial propagation of this medicinal plant. It is necessary to conduct additional research in order to optimize the composition, EC and pH value of the hydroponic nutrient solution.

scholarly journals Improved micropropagation and foliar micromorphological studies in Turnera ulmifolia L. – An important medicinal plant

2018 ◽  
Vol 30 (2) ◽  
pp. 283-294 ◽  
Author(s):  
Mani Manokari ◽  
Mahipal S. Shekhawat
Keyword(s):  
Shoot Proliferation ◽  
Nodal Segments ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Half Strength ◽  
Turnera Ulmifolia ◽  
Semi Solid ◽  
Number Of Shoots

Abstract The present study reports an efficient in vitro propagation system for Turnera ulmifolia using nodal segments as explants. Turnera ulmifolia (Passifloraceae) is an important garden plant with multipotent medicinal values. Effective shoot proliferation was achieved on agar gelled MS medium (Murashige and Skoog, 1962). The maximum number of shoots (8.3 ± 0.57) per initial explant was obtained on MS medium supplemented with 8.88 mM of 6-benzylaminopurine (BAP) and 0.54 mM of α-naphthalene acetic acid (NAA). The highest number of shoots (59.5 ± 2.10) proliferated on semi-solid MS medium (with agar) augmented with 2.22 mM of BAP and 2.32 mM of kinetin (Kin) along with 0.54 mM of NAA. Longer (4-5 cm) and healthy shoots were rooted (12.0 ± 0.10 roots per shoot) on half-strength MS medium fortified with 9.84 mM of indole-3 butyric acid (IBA). The in vitro regenerated plantlets were hardened in the greenhouse and transferred to the field. Significant developmental changes were observed in the foliar micromorphology of in vitro raised plantlets when these were transferred to the field. The stomatal index was gradually reduced (26.72 to 21.25) in the leaves from in vitro to field environments. But, vein-islets and veinlet terminations (13.4 and 7.6) were increased (39.7 and 18.4) respectively from in vitro to in vivo grown plants. Simple, unicellular, less frequent and underdeveloped trichomes were observed with the leaves of in vitro plants but fully developed trichomes recorded in the field transferred plants. The study could help in understanding the response and adaptation of tissue culture raised plantlets towards changed environmental conditions.

scholarly journals Transformation of Nicotiana alata Link and Otto. with an Autoregulatory Senescence-inhibitor Gene Construct

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 617c-617
Author(s):  
Kenneth R. Schroeder ◽  
Dennis P. Stimart
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Nicotiana Alata ◽  
Ms Medium ◽  
Gene Encoding ◽  
Half Strength ◽  
Cytokinin Synthesis ◽  
Senesced Leaves

Leaf explants of Nicotiana alata Link and Otto. were surface disinfested and cultured on Murashige and Skoog (MS) medium containing 2.66 μm N6-benzyladenine (BA) to promote shoot proliferation. After 5 weeks, proliferated shoots were removed and remaining callus saved. Callus was inoculated with Agrobacterium tumefaciens encoding a senescence-specific promoter SAG12 cloned from Arabidopsis thaliana fused to a Agrobacterium tumefaciens gene encoding isopentenyl transferase which catalyzes cytokinin synthesis. Following inoculation, the callus was cocultivated for 6 days on BA medium. Selection for transgenics was done on BA medium plus 100 mg Kanamycin and 400 mg Ticarcillin (antibiotics) per liter. Proliferating shoots were rooted on MS medium containing antibiotics. Rooted cuttings were transplanted to soil, acclimated and flowered in the greenhouse. Transgenics were outcrossed to a commercial N. alata hybrid. Seed was germinated in vitro on half-strength MS medium plus antibiotics. Segregation of transgenics to nontransgenics was 1:1. Evaluation of leaf senescence on 5-month-old plants showed 2 to 14 times fewer senesced leaves on the transgenic than the nontransgenic plants.

scholarly journals In vitro clonal propagation and evaluation of antibacterial activity of Benghal dayflower-Commelina benghalensis L.

2020 ◽  
Vol 12 (3) ◽  
pp. 628-636
Author(s):  
Md. M. ISLAM ◽  
Md. A. AL MAMUN ◽  
Md. F. ALAM
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Species ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Ms Medium ◽  
Commelina Benghalensis ◽  
Half Strength ◽  
Ethanol Extracts ◽  
Benghal Dayflower

An efficient protocol for in vitro propagation of Commelina benghalensis was developed. Nodal segments were showed the superb explants in performance for shoot proliferation than other explants. On the other hand, BAP with auxin was better combination. The maximum (90.52) number of explants response and the highest (23.25) number of shoots per plant were obtained from nodal segments on MS medium fortified with 3.0 mg/l BAP+0.1 mg/l IBA. The highest shoot length (17.25 cm) was achieved on MS medium containing 3.0mg/l BAP+0.5mg/l IBA. In vitro proliferated shoots were transferred to full and half strength of MS media where 1.5 mg/l IBA on full strength of MS media was the best to fit for the maximum number (12.69) of roots formation per micro-shoot. Well rooted plantlets were transferred to soil and successfully acclimatized with 97% survival rate. Three extracts i.e. methanol, ethanol, Petroleum ether of C. benghalensis L and four concentrations of each extracts were used against five gram (+ve) and five gram (-ve) bacterial species for the screening of antibacterial activity. Ethanol extracts was the superior in performance. The susceptibility of tested pathogenic bacterial species was increasing compare with increasing of extracts concentration with few exceptions. The highest zone of inhibition was obtained against S. aureus (17.50 mm) and P. aeruginosa (17.44 mm) at 800 mg/l dose level of extracts. It was also noticed that Gram (+ve) bacterial species are more susceptible to Benghal dayflower crude extracts than Gram (-ve) bacterial species.

scholarly journals SHOOT PROLIFERATION OF CERCIS CANADENSIS L. IN VITRO USING THIDIAZURON AND BENZYLADENINE.

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1088e-1088
Author(s):  
Len Burkhart ◽  
Martin Meyer
Keyword(s):  
Success Rate ◽  
Shoot Proliferation ◽  
Shoot Tip ◽  
Ms Medium ◽  
Cercis Canadensis ◽  
Shoot Number ◽  
Modified Ms Medium ◽  
High Concentrations

Selected cultivars of redbud (Cercis canadensis L.) and related Cercis species are usually propagated by grafting, but the success rate is low and other problems can be associated with the rootstock. Micropropagation would solve many of these problems. Shoots from a 25 year-old redbud were collected during July 1989 and established in vitro on modified MS medium. Shoots proliferated poorly with lower concentrations of Benzyladenine (BA) and high concentrations of BA caused shoot tip abortion. Similar problems with red-silver hybrid maples were solved by the use of Thidiazuron (TZ) in the medium. Established 2 cm redbud shoots were treated with TZ (0, 0.05, and 0.1 uM) and BA (0, 1 and 5 uM) in a factorial arrangement to test for shoot proliferation. After 4 weeks of the treatment with 0.1 uM TZ and 5 uM BA, mean shoot number was 4.6 compared to 1.1 shoots with no BA or TZ in the medium. Further experiments with rooting treatments will be presented.

scholarly journals In vitro Regeneration of Saussurea heteromalla (D. Don.) Hand-Mazz Using Auxins and Current Status in Galiyat Abbottabad

2020 ◽  
Vol 10 (5) ◽  
pp. 1-7
Author(s):  
H. Mehreen ◽  
J. Zafar ◽  
G. Zishan
Keyword(s):  
In Vitro Regeneration ◽  
In Vitro Rooting ◽  
Current Status ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Indole Butyric Acid ◽  
Half Strength ◽  
Emergence Percentage

Current Status of Saussurea heteromalla was investigated in Galiyat areas of District Abbottabad, viz., Jahaffar, Seri, Beeran Gali, Banj, Haryala, Daryala Gali, Sarbhanna, Barriyan, Akhreela and Broangiala. Saussurea heteromalla was found common in Seri, Sarbhanna and Barriyan; endangered in Haryala, Jahaffar, Banj, Daryala Gali and Beeran Gali and absent in Akhreela and Broangiala. In vitro regeneration of Saussurea heteromalla (D. Don.) Hand-Mazz on MS media was conducted and shoots were developed on full strength MS medium supplemented with 1 mgL-1 GA3. The developed shoots were transferred for root induction to half strength MS medium fortified with various concentrations of Indole butyric acid (IBA) and α-Naphthalene acetic acid (NAA) i.e. T1 (control), T2 (2 mgL-1 IBA), T3 (3 mgL-1 IBA), T4 (4 mgL-1 IBA), T5 (1 mgL-1 NAA), T6 (2 mgL-1 NAA) and T7 (3 mgL-1 NAA). Maximum mean shoot length (6.3 cm), mean number of leaves (7), mean number of nodes (5.25); highest root emergence percentage (71%), means root length (1.5 cm), mean number of roots (3) and highest survival rate (100%) was recorded in treatment T4. However, treatments T5 and T6 also seem to be effective for in vitro rooting of Saussurea heteromalla. Treatments T2, T3 and T7 showed minimum root growth. It was concluded that IBA at higher concentration is more effective for in vitro rooting and better shoot growth of Saussurea heteromalla whereas NAA also initiate rooting but at lower concentration.

Seed germination, micropropagation from adult and juvenile origin explants and address of hyperhydricity of the Cretan endemic herb Calamintha cretica

2020 ◽  
Vol 48 (3) ◽  
pp. 1504-1518
Author(s):  
Georgia VLACHOU ◽  
Maria Maria PAPAFOTIOU ◽  
Konstantinos Bertsouklis
Keyword(s):  
Shoot Proliferation ◽  
Shoot Length ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Optimum Range ◽  
Shoot Number ◽  
Half Strength ◽  
One Year ◽  
Cardinal Temperatures

The optimum range of temperature for germination (96-100%) of Calamintha cretica, an herb with potential pharmaceutical and horticultural uses, was 15 to 20 °C, with 10 and 30 °C cardinal temperatures. Storage up to one year did not affect germination. The effect of zeatin (ZEA), 6-benzyladenine (BA), kinetin, and 6-γ-γ-(dimethylallylamino)-purine added in MS medium at concentrations from 0.0 to 8.0 mg L-1 was tested for shoot proliferation of both adult- and seedling-origin nodal explants at first- and sub-culture. Both explant types responded similarly during in vitro culture. At cytokinin concentrations up to 1 mg L-1 explant response was high (over 85%) but shoot number per explant was low (1.2-2.2). Increasing cytokinin from 2.0 to 8.0 mg L-1 resulted to an analogous decrease of explant response and shoot length, and an increase of shoot number, particularly when ZEA or BA was used (5.0-6.6 shoots per explant, 0.5-1.0 cm long) with simultaneous though increase of hyperhydricity (up to 50%). The addition of 0.1 mg L-1 naphthaleneacetic acid into the 8.0 mg L-1 BA medium almost eliminated hyperhydricity and increased explant response, while the increase of agar concentration from 8.0 to 12.0 g L-1 eliminated hyperhidricity and induced the highest shoot proliferation (93-95% explant response, 11.2-12.3 shoots per explant, 0.8-1.0 cm long). Microshoots and microshoot clusters rooted (88-96%) on half-strength MS medium either hormone free or supplemented with 1 to 4 mg L-1 indole-3-butyric acid. Plantlets survived at 80% to 100% after ex vitro acclimatization in peat: perlite 1:1 (v/v).

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