scholarly journals SHOOT PROLIFERATION OF CERCIS CANADENSIS L. IN VITRO USING THIDIAZURON AND BENZYLADENINE.

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1088e-1088
Author(s):  
Len Burkhart ◽  
Martin Meyer
Keyword(s):  
Success Rate ◽  
Shoot Proliferation ◽  
Shoot Tip ◽  
Ms Medium ◽  
Cercis Canadensis ◽  
Shoot Number ◽  
Modified Ms Medium ◽  
High Concentrations

Selected cultivars of redbud (Cercis canadensis L.) and related Cercis species are usually propagated by grafting, but the success rate is low and other problems can be associated with the rootstock. Micropropagation would solve many of these problems. Shoots from a 25 year-old redbud were collected during July 1989 and established in vitro on modified MS medium. Shoots proliferated poorly with lower concentrations of Benzyladenine (BA) and high concentrations of BA caused shoot tip abortion. Similar problems with red-silver hybrid maples were solved by the use of Thidiazuron (TZ) in the medium. Established 2 cm redbud shoots were treated with TZ (0, 0.05, and 0.1 uM) and BA (0, 1 and 5 uM) in a factorial arrangement to test for shoot proliferation. After 4 weeks of the treatment with 0.1 uM TZ and 5 uM BA, mean shoot number was 4.6 compared to 1.1 shoots with no BA or TZ in the medium. Further experiments with rooting treatments will be presented.

IN VITRO RESPONSE BY Terminalia arjuna GENOTYPES DURING MICROPROPAGATION

2021 ◽  
Vol 9 (1) ◽  
pp. 44-50
Author(s):  
Meena Choudhary ◽  
◽  
Ashok Gehlot ◽  
Sarita Arya ◽  
Inder Dev Arya ◽  
...  
Keyword(s):  
Shoot Proliferation ◽  
In Vitro Rooting ◽  
Terminalia Arjuna ◽  
Ms Medium ◽  
Demand And Supply ◽  
Half Strength ◽  
Modified Ms Medium ◽  
In Vitro Response ◽  
Different Doses

Terminalia arjuna is an important tree of the medicinal and sericulture industry, commonly known as Arjun. It’s bark is rich in secondary metabolites makes this plant highly valuable in medicine industry to treat cardiovascular disease. Overexploitation due to high demand in medicine, low seed germination, limitations of the conventional method of propagation push this plant towards being endangered. To conserve germplasm of such tree species and meet the requirement in medicinal industry, some non-conventional propagation method like micropropagation has been developed. The present work highlighted the effect of three genotypes (G-1, G-2, and G-3) on tissue culture of T. arjuna situated at Jodhpur, Rajasthan, India. In vitro shoot proliferation was achieved on a modified MS medium enriched with BAP + additives. Among the tested genotypes, Genotype -1 showed maximum bud break response (100%) followed by G-3 (93.33 %) and G-2 (86.66%). Further multiplication of these shoots on modified MS medium containing BAP + NAA + additives gave 11.38±0.26 (G-1), 9.44±0.21 (G-2) and 10.22±0.32 (G-3) shoots. In vitro rooting was done by pulse treatment with IBA for 10 min prior to transfer on hormone free half strength MS medium containing 0.1% activated charcoal. Maximum in vitro rooting was obtained in G-1 (80%) followed by G-3 (71.11%) and G-2 (68.88%). In the present study, it was observed that optimum growth in all three genotypes required different doses of Plant Growth Regulator. Thus, by identifying and multiplying the best performing genotypes the gap between demand and supply of such medicinal plant can be fulfilled.

scholarly journals Influence of the Genotype and TDZ on the in Vitro Regeneration of Hybrid Grapes

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 877B-877
Author(s):  
Maritza I. Tapia ◽  
Paul E Read
Keyword(s):  
In Vitro Regeneration ◽  
Shoot Proliferation ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Ms Medium ◽  
High Concentrations ◽  
Grape Cultivars

It has been previously demonstrated that thidiazuron (TDZ) enhanced the regeneration and multiple shoot proliferation of vinifera grape cultivars. To determine the effect of TDZ on the multiplication of hybrid grapes, in vitro nodal segments from cultivars Chancellor, Leon Millot, and Valiant were cultured on MS medium supplemented with 0, 0.01, 0.05, 0.1, 0.5, and 1.0 mg TDZ/liter. After 1 month, the higher percentage of rooted shoots was obtained from the explants cultured in medium containing the lowest concentration of TDZ (0.01 mg–liter–1) independent of the genotype. Multiple shoot proliferation was favored by high concentrations of TDZ (0.5 and 1.0 mg–liter–1). An average of 0.39 and 0.39 shoots, respectively, was obtained from `Chancellor' cultures, 0.56 and 0.59 from `Leon Millot', and 1.93 and 2.38 from `Valiant'. Vitrification and teratological structures were observed in all the cultures of the three genotypes, but less vitrification occurred in `Valiant' plantlets.

scholarly journals Micropropagation of the Rare Lakeside Daisy (Hymenoxys acaulis var. glabra)

HortScience ◽  
2002 ◽  
Vol 37 (1) ◽  
pp. 200-201 ◽  
Author(s):  
James R. Ault
Keyword(s):  
Overall Survival ◽  
Butyric Acid ◽  
Potassium Salt ◽  
Shoot Proliferation ◽  
Shoot Tip ◽  
Stem Segment ◽  
Ms Medium ◽  
Benzyl Adenine ◽  
Shoot Initiation

Shoot tip and stem segment explants collected from greenhouse-maintained plants of Hymenoxys acaulis var. glabra were cultured in vitro for shoot initiation on a Murashige and Skoog (MS) medium supplemented with 30 g·L-1 sucrose, 2.5 μm BA, and 7 g·L-1 agar at a pH of 5.7. Unbranched shoot explants were subcultured to MS medium with 0.0, 0.5, 1, 2, 4 or 8 μm BA for shoot proliferation. A maximum of 10.3 shoots per explant was produced on the medium with 2.0 μm BA. Nonrooted shoots were subcultured to MS medium with 0.0, 0.5, 2, or 8 μm K-IBA for rooting. Maximum rooting was 90% on MS medium with 0.5 μm K-IBA. Rooted shoots were greenhouse-acclimatized for 10 days. Overall survival was 75%. Chemical names used: 6-benzyl adenine (BA); potassium salt of indole-3-butyric acid (K-IBA).

scholarly journals Influence of High Concentrations of Copper Sulfate on In Vitro Adventitious Organogenesis of Cucumis sativus L.

2021 ◽  
Author(s):  
Jorge Fonseca Miguel
Keyword(s):  
Copper Sulfate ◽  
Fold Increase ◽  
Shoot Formation ◽  
Induction Medium ◽  
Ms Medium ◽  
Cucumis Sativus L ◽  
Shoot Number ◽  
High Concentrations ◽  
Shoot Induction Medium

AbstractThe effects of different concentrations of copper sulfate (0.2 to 5 mg L−1) on in vitro callus and shoot formation of cucumber was investigated. Four-day-old cotyledon explants from the inbred line ‘Wisconsin 2843’ and the commercial cultivars ‘Marketer’ and ‘Negrito’ were used. The results on callus-derived shoots showed that the optimal concentration of CuSO4 added to Murashige & Skoog (MS)-derived shoot induction medium containing 0.5 mg L−1 IAA and 2.5 mg L−1 BAP was 8-200 fold greater than in standard MS medium, and was genotype dependent. The highest genotypes response on shoot frequency and shoot number was achieved in this order by ‘Marketer’, ‘Negrito’ and ‘Wisconsin 2843’ with 1, 0.2 y 5 mg L−1 CuSO4, respectively. The genotype with the lowest control performance demanded the highest concentration of CuSO4 for its optimal morphogenic response - 6- and 10-fold more in shoot frequency and shoot number, respectively. The other cultivars registered a 2-fold increase in both variables. All explants formed callus and the response on callus extension varied among cultivars. The regression analysis showed a statistically significant relationship between shoot number and concentrations of CuSO4 and absence of association with callus extension. The present results indicate that application of specific concentrations of CuSO4 higher than in standard MS medium, increases adventitious cucumber shoot organogenesis.

scholarly journals Thidiazuron and Benzylamino Purine Stimulation of Apple Shoot Proliferation in Vitro

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 629e-629
Author(s):  
Karim H. Al-Juboory ◽  
Jabar H. Al-Niami
Keyword(s):  
Malus Domestica ◽  
Shoot Proliferation ◽  
Shoot Tip ◽  
Ms Medium ◽  
Proliferation In Vitro ◽  
Shoot Tip Explants ◽  
Apple Shoot ◽  
Stimulation Of

Thidiazuron (TDZ) and benzylamino purine stimulated shoot proliferation on shoot tip explants of wild apple (Malus domestica Borkh) when incorporated in Murashige and Skoog (MS) medium at concentrations of 1.0–10 μm. Shoot numbers obtained with TDZ were greater than the number produced when using BA in the medium but the shoots were shorter than with BA. Increasing TDZ levels increased shoot proliferation with 10 μm. Apple shoots were successfully rooted on MS medium with 2.0 mg·L–1 NAA and then transferred to a mixture of 1 peat: 1 perlite: 1 soil and acclimatized for potting.

Seed germination, micropropagation from adult and juvenile origin explants and address of hyperhydricity of the Cretan endemic herb Calamintha cretica

2020 ◽  
Vol 48 (3) ◽  
pp. 1504-1518
Author(s):  
Georgia VLACHOU ◽  
Maria Maria PAPAFOTIOU ◽  
Konstantinos Bertsouklis
Keyword(s):  
Shoot Proliferation ◽  
Shoot Length ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Optimum Range ◽  
Shoot Number ◽  
Half Strength ◽  
One Year ◽  
Cardinal Temperatures

The optimum range of temperature for germination (96-100%) of Calamintha cretica, an herb with potential pharmaceutical and horticultural uses, was 15 to 20 °C, with 10 and 30 °C cardinal temperatures. Storage up to one year did not affect germination. The effect of zeatin (ZEA), 6-benzyladenine (BA), kinetin, and 6-γ-γ-(dimethylallylamino)-purine added in MS medium at concentrations from 0.0 to 8.0 mg L-1 was tested for shoot proliferation of both adult- and seedling-origin nodal explants at first- and sub-culture. Both explant types responded similarly during in vitro culture. At cytokinin concentrations up to 1 mg L-1 explant response was high (over 85%) but shoot number per explant was low (1.2-2.2). Increasing cytokinin from 2.0 to 8.0 mg L-1 resulted to an analogous decrease of explant response and shoot length, and an increase of shoot number, particularly when ZEA or BA was used (5.0-6.6 shoots per explant, 0.5-1.0 cm long) with simultaneous though increase of hyperhydricity (up to 50%). The addition of 0.1 mg L-1 naphthaleneacetic acid into the 8.0 mg L-1 BA medium almost eliminated hyperhydricity and increased explant response, while the increase of agar concentration from 8.0 to 12.0 g L-1 eliminated hyperhidricity and induced the highest shoot proliferation (93-95% explant response, 11.2-12.3 shoots per explant, 0.8-1.0 cm long). Microshoots and microshoot clusters rooted (88-96%) on half-strength MS medium either hormone free or supplemented with 1 to 4 mg L-1 indole-3-butyric acid. Plantlets survived at 80% to 100% after ex vitro acclimatization in peat: perlite 1:1 (v/v).

scholarly journals An EfficientIn VitroPlantlet Regeneration from Shoot Tip Cultures ofCurculigo latifolia, a Medicinal Plant

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Nahid Babaei ◽  
Nur Ashikin Psyquay Abdullah ◽  
Ghizan Saleh ◽  
Thohirah Lee Abdullah
Keyword(s):  
Medicinal Plant ◽  
Butyric Acid ◽  
Shoot Length ◽  
Shoot Tip ◽  
Direct Regeneration ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Tip Culture ◽  
Shoot Number

A procedure was developed forin vitropropagation ofCurculigo latifoliathrough shoot tip culture. Direct regeneration and indirect scalp induction ofCurculigo latifoliawere obtained from shoot tip grown on MS medium supplemented with different concentrations and combinations of thidiazuron and indole-3-butyric acid. Maximum response for direct regeneration in terms of percentage of explants producing shoot, shoot number, and shoot length was obtained on MS medium supplemented with combination of thidiazuron (0.5 mg L−1) and indole-3-butyric acid (0.25 mg L−1) after both 10 and 14 weeks of cultures. Indole-3-butyric acid in combination with thidiazuron exhibited a synergistic effect on shoot regeneration. The shoot tips were able to induce maximum scalp from basal end of explants on the medium with 2 mg L−1thidiazuron. Cultures showed that shoot number, shoot length, and scalp size increased significantly after 14 weeks of culture. Transferring of the shoots onto the MS medium devoid of growth regulators resulted in the highest percentage of root induction and longer roots, while medium supplemented with 0.25 mg L−1IBA produced more numbers of roots.

scholarly journals In Vitro Clonal Propagation of Nardostachys jatamansi: A Traditional Himalayan Medicinal Plant

2021 ◽  
Vol 16 (3) ◽  
Author(s):  
Hem Chandra Pant ◽  
Harsh Vardhan Pant ◽  
Arun Kumar ◽  
Himani Tomar ◽  
Manish Dev Sharma ◽  
...  
Keyword(s):  
Tap Water ◽  
Shoot Proliferation ◽  
Culture Method ◽  
Multiple Shoot ◽  
Shoot Tip ◽  
Nodal Explants ◽  
Garden Soil ◽  
Ms Medium ◽  
Bud Induction

Plant tissue culture method has an impressive technique for Investigation and Explains basic and applied problems in plant biotechnology field. Micropropagation has played a vital role in the rapid multiplication of many plants species. The nodal explants and shoot tip of N. jatamansi inoculated in MS medium (Murashige and Skoog) contain different types concentrations of PGRs (Phytohormones) at various frequencies for the optimization of growth quality for shoot bud Induction, shoot proliferation and micro rooting in plant. The perfect shoot induction takes place in the concentration of BAP + IBA (2.0 mg/l +1.5 mg/1) multiplication of nodal explants and shoot tip in the combination lower concentration of BAP and KN (2.0 mg/1+1.5 mg/1) This combination proved best for multiple shoot formation. Half strength (1/2) of the MS medium containing NAA and BAP (1.5 mg/1+1.0 mg/1) in combination was most useful for rooting in plant. Well developed rooted micro shoots were smoothly removed for the culture flask and dipped in 70% ethanol for 2 min and then washed with running tap water for 5-10 min to remove media for the root and transferred to small plastic cups carry cocopeat, garden soil and sand (2:2:1) and produce healthy growth in ex-vitro conditions.

scholarly journals Improved Micropropagation of Plantlets from Nodal Explants of Anchote (Coccinia abyssinica)—A Calcium- and Protein-rich Tuber

HortScience ◽  
2016 ◽  
Vol 51 (7) ◽  
pp. 905-909 ◽  
Author(s):  
Jane Kahia ◽  
Peter Njenga ◽  
Margaret Kirika
Keyword(s):  
Shoot Proliferation ◽  
Calcium Content ◽  
Nodal Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Endemic Plant ◽  
Time Duration ◽  
High Calcium ◽  
High Concentrations

The effect of phytohormones on breaking of dormancy of axillary buds in anchote and their subsequent shoot proliferation were examined. Anchote is an annual trailing endemic plant with high calcium content grown for its edible tuberous roots in Ethiopia. Nodal explants harvested from the greenhouse were sterilized using various concentrations of a commercial bleach (JIK) which contains 3.85% sodium hypochlorite (NaOCl) and time duration. The highest (85%) clean explants were obtained when 5% JIK was used for 15 minutes. The explants were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of benzylaminopurine (BAP), kinetin, and thidiazuron (TDZ). The highest frequency of microshoot induction (84%) and mean number of microshoots (3.4) were recorded from explants cultured on medium supplemented with TDZ 0.025 µm. Hyperhydrated shoots were observed on media supplemented with high concentrations of BAP and kinetin but interestingly not on TDZ media. Induction of roots was highest (86%; 4.6 roots per shoot) when shoots were transferred to half strength MS medium containing 0.5 μm α-naphthalene acetic acid (NAA) after 12 days. A survival rate of 83% was recorded in the greenhouse and the plantlets appeared to be morphologically normal. This is the first report on use of TDZ for in vitro propagation of anchote.

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