Crecimiento de Trichoderma asperellum en medio sólido utilizando como única fuente de carbono a los plaguicidas clorpirifos y cipermetrin

2020 ◽  
Vol 11 (2) ◽  
pp. 149-156
Author(s):  
Miguel Ángel Muñoz Ríos ◽  
Walter Andres Rojas Villacorta ◽  
Ingrid Lisset Malqui Ramos
Keyword(s):  
Trichoderma Asperellum

El objetivo de esta investigación fue evaluar el crecimiento de Trichoderma asperellum en medio sólido utilizando como única fuente de carbono a los plaguicidas cipermetrina (piretroide) y clorpirifos (organofosforado). Para este propósito, se realizaron cultivos de T. asperellum sobre el medio Agar Mínimo de Sales, los cuales tenían como únicas fuentes de carbono a los plaguicidas cipermetrina (480 ppm) y clorpirifos (250 ppm). Posteriormente los cultivos fueron incubados a 25 ºC por 5 días. Finalmente se calcularon las velocidades de crecimiento. El control tuvo como única fuente de carbono a la glucosa. Los resultados muestran que T. asperellum puede crecer en ambos medios utilizando a los plaguicidas como una sola fuente de carbono y en cuanto a sus velocidades de crecimiento fueron 2.88 ± 0.05 mm/día (cipermetrina) y 2.74 ± 0.05 mm/día (clorpirifos). En conclusión, T. asperellum es capaz de utilizar a los plaguicidas cipermetrina y clorpirifos mediante procesos catabólicos y tiene el potencial de ser usado en biorremediación de plaguicidas de suelos contaminados.

scholarly journals Co-culture of Vel1-overexpressed Trichoderma asperellum and Bacillus amyloliquefaciens: An eco-friendly strategy to hydrolyze the lignocellulose biomass in soil to enrich the soil fertility, plant growth and disease resistance

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Valliappan Karuppiah ◽  
Lu Zhixiang ◽  
Hongyi Liu ◽  
Murugappan Vallikkannu ◽  
Jie Chen
Keyword(s):  
Disease Resistance ◽  
Plant Growth ◽  
Corn Stover ◽  
Bacillus Amyloliquefaciens ◽  
Genetically Engineered ◽  
Cellulolytic Enzymes ◽  
Regulation Of Transcription ◽  
Trichoderma Asperellum ◽  
Biomass Hydrolysis ◽  
Lignocellulose Biodegradation

Abstract Background Retention of agricultural bio-mass residues without proper treatment could affect the subsequent plant growth. In the present investigation, the co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens has been employed for multiple benefits including the enrichment of lignocellulose biodegradation, plant growth, defense potential and disease resistance. Results The Vel1 gene predominantly regulates the secondary metabolites, sexual and asexual development as well as cellulases and polysaccharide hydrolases productions. Overexpression mutant of the Trichoderma asperellum Vel1 locus (TA OE-Vel1) enhanced the activity of FPAase, CMCase, PNPCase, PNPGase, xylanase I, and xylanase II through the regulation of transcription regulating factors and the activation of cellulase and xylanase encoding genes. Further, these genes were induced upon co-cultivation with Bacillus amyloliquefaciens (BA). The co-culture of TA OE-Vel1 + BA produced the best composition of enzymes and the highest biomass hydrolysis yield of 89.56 ± 0.61%. The co-culture of TA OE-Vel1 + BA increased the corn stover degradation by the secretion of cellulolytic enzymes and maintained the C/N ratio of the corn stover amended soil. Moreover, the TA OE-Vel1 + BA increased the maize plant growth, expression of defense gene and disease resistance against Fusarium verticillioides and Cohilohorus herostrophus. Conclusion The co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens could be utilized as a profound and meaningful technique for the retention of agro residues and subsequent plant growth.

scholarly journals Approving the biocontrol method of potato wilt caused by Ralstonia solanacearum (Smith) using Enterobacter cloacae PS14 and Trichoderma asperellum T34

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Bereika F. F. Mohamed ◽  
Nashwa M. A. Sallam ◽  
Saad A. M. Alamri ◽  
Kamal A. M. Abo-Elyousr ◽  
Yasser S. Mostafa ◽  
...  
Keyword(s):  
Ralstonia Solanacearum ◽  
Enterobacter Cloacae ◽  
Trichoderma Asperellum ◽  
Potato Wilt

Bi-model cationic dye adsorption by native and surface-modified Trichoderma asperellum BPL MBT1 biomass: From fermentation waste to value-added biosorbent

Chemosphere ◽  
2021 ◽  
pp. 130311
Author(s):  
Sabarathinam Shanmugam ◽  
Kumaravel Karthik ◽  
Udayakumar Veerabagu ◽  
Anjana Hari ◽  
Krishnaswamy Swaminathan ◽  
...  
Keyword(s):  
Dye Adsorption ◽  
Value Added ◽  
Cationic Dye ◽  
Trichoderma Asperellum ◽  
Surface Modified

scholarly journals Trichoderma asperellum T76-14 Released Volatile Organic Compounds against Postharvest Fruit Rot in Muskmelons (Cucumis melo) Caused by Fusarium incarnatum

2021 ◽  
Vol 7 (1) ◽  
pp. 46
Author(s):  
Warin Intana ◽  
Suchawadee Kheawleng ◽  
Anurag Sunpapao
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Cucumis Melo ◽  
Fungal Growth ◽  
Fruit Rot ◽  
Postharvest Disease ◽  
Dual Culture ◽  
Trichoderma Asperellum ◽  
Volatile Organic

Postharvest fruit rot caused by Fusarium incarnatum is a destructive postharvest disease of muskmelon (Cucumis melo). Biocontrol by antagonistic microorganisms is considered an alternative to synthetic fungicide application. The aim of this study was to investigate the mechanisms of action involved in the biocontrol of postharvest fruit rot in muskmelons by Trichoderma species. Seven Trichoderma spp. isolates were selected for in vitro testing against F. incarnatum in potato dextrose agar (PDA) by dual culture assay. In other relevant works, Trichoderma asperellum T76-14 showed a significantly higher percentage of inhibition (81%) than other isolates. Through the sealed plate method, volatile organic compounds (VOCs) emitted from T. asperellum T76-14 proved effective at inhibiting the fungal growth of F. incarnatum by 62.5%. Solid-phase microextraction GC/MS analysis revealed several VOCs emitted from T. asperellum T76-14, whereas the dominant compound was tentatively identified as phenylethyl alcohol (PEA). We have tested commercial volatile (PEA) against in vitro growth of F. incarnatum; the result showed PEA at a concentration of 1.5 mg mL−1 suppressed fungal growth with 56% inhibition. Both VOCs and PEA caused abnormal changes in the fungal mycelia. In vivo testing showed that the lesion size of muskmelons exposed to VOCs from T. asperellum T76-14 was significantly smaller than that of the control. Muskmelons exposed to VOCs from T. asperellum T76-14 showed no fruit rot after incubation at seven days compared to fruit rot in the control. This study demonstrated the ability of T. asperellum T76-14 to produce volatile antifungal compounds, showing that it can be a major mechanism involved in and responsible for the successful inhibition of F. incarnatum and control of postharvest fruit rot in muskmelons.

Effects of Trichoderma asperellum on Germination Indexes and Seedling Parameters of Lettuce Cultivars

2021 ◽  
Vol 79 (1) ◽  
Author(s):  
Rafael Rodrigues de Souza ◽  
Mariana Poll Moraes ◽  
João Antônio Paraginski ◽  
Thainá Fogliatto Moreira ◽  
Karina Chertok Bittencourt ◽  
...  
Keyword(s):  
Trichoderma Asperellum

scholarly journals Evaluation of biological efficacy of Trichoderma asperellum against tomato bacterial wilt caused by Ralstonia solanacearum

2018 ◽  
Vol 28 (1) ◽  
Author(s):  
Narasimhamurthy Konappa ◽  
Soumya Krishnamurthy ◽  
Chandra Nayaka Siddaiah ◽  
Niranjana Siddapura Ramachandrappa ◽  
Srinivas Chowdappa
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Trichoderma Asperellum ◽  
Biological Efficacy ◽  
Tomato Bacterial Wilt

Effect of Nanoparticles on the Growth of Micromycetes: The Case of Trichoderma Asperellum

2018 ◽  
Vol 13 (3-4) ◽  
pp. 205-210
Author(s):  
O. D. Smirnova ◽  
K. V. Palamarchuk ◽  
I. V. Kalashnikova ◽  
V. Yu. Musatova ◽  
S. A. Semenov
Keyword(s):  
Trichoderma Asperellum
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