Background:One of the most common comorbidity in patients with rheumatoid arthritis (RA) is arterial hypertension (AH), with incidence ranging from 20 to 60%. Mechanisms of this comorbidity arises a lot of interest. In our previous study was established the association ofT786C NOS3(rs2070744) gene polymorphism with AH in females with RA in the Ukrainian population [1].Objectives:So next, we were aiming to investigate daily fluctuation of endothelial nitric oxide synthase (NOS3) in RA patients with AH depending onNOS3 T786Cgene polymorphism.Methods:In the study were enrolled 173 females with RA aged 43.7 ± 7.35 years (Mean ± SD) and 34 age-matched healthy women without joint diseases and autoimmune diseases (control). Serum NOS3 level was determined at 08:00 and 20:00 using Cloud-Clone Corp kits (USA).NOS3 T-786Сpolymorphism was determined by Real-Time PCR (Bio-Rad iCycler IQ5) using SNP-express kit. Study was carried out in compliance with bioethical standards and provisions of the WHO, Helsinki Declaration of the General Assembly of the World Medical Association (1989).Results:Among enrolled patients prevailed individuals with more than 5 years disease history, ІІ-ІІІ radiographic stage (80.9 %), and were seropositive for anti-cyclic citrullinated peptide (80.6%). There were 114 (66%) normotensive patients and 59 (34%) patients with AH (13% - I stage, 20.8% - II stage). The daily fluctuation of NOS3 serum level was established in the control group. The evening NOS3 level was higher in 1.3 times, than the morning level (р<0.001). In RA patients the similar fluctuations of NOS3 level was registered, but the daily NOS3 production was lower, than in control. Diurnal variation of NOS3 level depended on comorbid AH andNOS3 T-786Сgenotype. InCCgenotype NOS3 levels at 08:00 and at 20:00 were lower in 1.2-1.3 times (p<0.05) than inTTandTCgenotypes. In patients with RA and AH the lowest diurnal variation of NOS3 level was inCCgenotype. The decrease of evening NOS3 production was strong associated with comorbid AH (OR 3.78; 95% CI 1.96-7.28).Conclusion:Circadian rhythms of NOS3 production in females with RA and AH depend onNOS3 Т786Сgene polymorphism. The depression of NOS3 production in the evening can be predictor of comorbid AH in females with RA.References:[1]Zaichko, K., & Stanislavchuk, M. (2017, April). T-786C gene promoter polymorphism of endothelial nitric oxide synthase: its association to the disease activity and arterial hypertension in patients with rheumatoid arthritis in the ukraine population. In rheumatology (vol. 56, pp. 129-129). GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND: OXFORD UNIV PRESS.Acknowledgments:NADisclosure of Interests:None declared
Possible genetically determined mechanisms of arterial hypertension in tobacco smoking
