Peptide phage display in biotechnology and biomedicine

To date peptide phage display is one of the most common combinatorial methods used for identifying specific peptide ligands. Phage display peptide libraries containing billions different clones successfully used for selection of ligands with high affinity and selectivity toward wide range of targets including individual proteins, bacteria, viruses, spores, different kind of cancer cells and variety of nonorganic targets (metals, alloys, semiconductors etc.) Success of using filamentous phage in phage display technologies relays on the robustness of phage particles and a possibility to genetically modify its DNA to construct new phage variants with novel properties. In this review we are discussing characteristics of the most known non-commercial peptide phage display libraries of different formats (landscape libraries in particular) and their successful applications in several fields of biotechnology and biomedicine: discovery of peptides with diagnostic values against different pathogens, discovery and using of peptides recognizing cancer cells, trends in using of phage display technologies in human interactome studies, application of phage display technologies in construction of novel nano materials

Download Full-text

Development of Anti-Infectives Using Phage Display: Biological Agents against Bacteria, Viruses, and Parasites

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00567-12 ◽

2012 ◽

Vol 56 (9) ◽

pp. 4569-4582 ◽

Cited By ~ 50

Author(s):

Johnny X. Huang ◽

Sharon L. Bishop-Hurley ◽

Matthew A. Cooper

Keyword(s):

Phage Display ◽

Significant Contribution ◽

Biological Agents ◽

Preclinical Development ◽

Phage Display Technology ◽

Whole Cells ◽

Drug Candidates ◽

Phage Display Libraries ◽

Wide Range ◽

Display Technology

ABSTRACTThe vast majority of anti-infective therapeutics on the market or in development are small molecules; however, there is now a nascent pipeline of biological agents in development. Until recently, phage display technologies were used mainly to produce monoclonal antibodies (MAbs) targeted against cancer or inflammatory disease targets. Patent disputes impeded broad use of these methods and contributed to the dearth of candidates in the clinic during the 1990s. Today, however, phage display is recognized as a powerful tool for selecting novel peptides and antibodies that can bind to a wide range of antigens, ranging from whole cells to proteins and lipid targets. In this review, we highlight research that exploits phage display technology as a means of discovering novel therapeutics against infectious diseases, with a focus on antimicrobial peptides and antibodies in clinical or preclinical development. We discuss the different strategies and methods used to derive, select, and develop anti-infectives from phage display libraries and then highlight case studies of drug candidates in the process of development and commercialization. Advances in screening, manufacturing, and humanization technologies now mean that phage display can make a significant contribution in the fight against clinically important pathogens.

Download Full-text