Evaluation of cytotoxic profile of hydroalcoholic extract of fruit rinds of Garcinia pedunculata on human embryonic kidney and breast carcinoma cells

Background: The fruit rinds of Garcinia pedunculata has potential medicinal properties and used in many chronic ailments. It has been demonstrated that cytoprotective effects in various experimental research works. But its cytotoxic effect has not been evaluated. The present study was aimed to screen its relative cytotoxic effect on normal and cancer cell lines.Methods: In the present study, the cytotoxic effect of hydro alcoholic extract of Garcinia pedunculata was evaluated on normal human embryonic kidney (HEK-293) and M.D. Anderson metastatic breast cancer cell lines (MDA-MB 231) using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay.Results: Higher dose level of hydro alcoholic extract of Garcinia pedunculata (HAGP) (500 μg/ml) has shown considerable increase (112.503) in the percentage viability of HEK-29 whereas; there is a remarkable decrease in the viable cell population (77.490) in MDA-MB 231.Conclusions: Based on the observed results we could conclude that HAGP has potential cytotoxic effect on the cancer cell line without altering the normal cell growth and proliferation. Thus it has potential to develop as a safer chemotherapeutic agent. Further detailed exploration is required to confirm its therapeutic efficacy in different cancer cell lines.

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Synthesis, Characterization and Biological Evaluation of Metal Adamantyl 2-Pyridylhydrazone Complexes

Molecules ◽

10.3390/molecules25112530 ◽

2020 ◽

Vol 25 (11) ◽

pp. 2530

Author(s):

Ihsan A. Shehadi ◽

Fatima-Azzahra Delmani ◽

Areej M. Jaber ◽

Hana Hammad ◽

Murad A. AlDamen ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Human Cancer ◽

Cancer Cell Line ◽

Direct Methods ◽

Cancer Cell Lines ◽

Biological Evaluation ◽

Colorectal Cancer Cell Line ◽

Breast Cancer Cell Lines ◽

Human Cancer Cell Lines

Four new complexes derived from adamantly containing hydrazone (APH) ligand with Cu(II) (1), Co(II) (2), Ni(II) (3) and Zn(II) (4), have been synthesized and characterized using different physicochemical methods. The structure of the ligand APH and its copper complex 1 have been established by single-crystal X-ray diffraction direct methods, which reveal that complex 1 has distorted square-pyramidal geometry. Complexes 1–4 are screened against seven human cancer cell lines namely, breast cancer cell lines (MCF7, T47D, MDA-MB-231), prostate cancer cell lines (PC3, DU145) and the colorectal cancer cell line Coco-2, for their antiproliferative activities. Complex 1 has shown a promising anticancer activity compared to the other ones. The structural and spectroscopic analysis of APH and its complexes are confirmed by DFT calculations.

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Targeting bortezomib-induced aggresome formation using vinorelbine enhances the cytotoxic effect along with ER stress loading in breast cancer cell lines

International Journal of Oncology ◽

10.3892/ijo.2016.3673 ◽

2016 ◽

Vol 49 (5) ◽

pp. 1848-1858 ◽

Cited By ~ 12

Author(s):

Kana Miyahara ◽

Hiromi Kazama ◽

Hiroko Kokuba ◽

Seiichiro Komatsu ◽

Ayako Hirota ◽

...

Keyword(s):

Breast Cancer ◽

Er Stress ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Cytotoxic Effect ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Aggresome Formation

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Differential Response to 1,25-dihydroxyvitamin D in Metastatic and Non-Metastatic Breast Cancer Cell Lines in Hypoxia (P05-006-19)

Current Developments in Nutrition ◽

10.1093/cdn/nzz030.p05-006-19 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Cited By ~ 1

Author(s):

Violet Kiesel ◽

Stephen Hursting ◽

Dorothy Teegarden

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Cell Lines ◽

Cancer Cell ◽

Mammary Cancer ◽

Metastatic Breast ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Dihydroxyvitamin D ◽

1,25 Dihydroxyvitamin D

Abstract Objectives Prevention of metastasis is of utmost importance for increasing survival in breast cancer patients. Oxygen tension is variable throughout tumors, creating regions of hypoxia that have been linked with poor cancer prognosis. Hypoxia increases glycolytic flux via hypoxia-inducible factor-1α (HIF1α), and can therefore alter growth and survival of cancer cells. Our objectives are to (1) characterize changes in metabolism and survival that occur when metastatic and non-metastatic mammary cancer cell lines are cultured in hypoxia, and (2) determine whether 1,25-dihydroxyvitamin D (1,25(OH)2D) reduces overall survival in hypoxia. Methods We utilized Wnt oncogene-driven murine mammary cancer cells that are non-metastatic (M-Wnt) or that preferentially metastasize to the lung in vivo (metM-Wntlung). Viability of M-Wnt and metM-Wntlung cells treated with 10 nM 1,25(OH)2D and/or 20 mM 2-deoxyglucose (2DG, an inhibitor of glycolysis) was measured with MTT. Expression of HIF1α protein was determined by Western blotting. Results We show that 1,25(OH)2D treatment significantly decreased viability of metastatic metM-Wntlung cells grown in hypoxia by 41%, whereas viability of M-Wnt cells was not significantly impacted by 1,25(OH)2D treatment. Furthermore, treating cells with 2DG significantly decreased viability of both cells lines in hypoxia, with metM-Wntlung cells being more sensitive to 2DG. Interestingly, 1,25(OH)2D treatment partially rescued M-Wnt cells by 22% and metM-Wntlung cells by 24% when treated with 2DG in hypoxia. Finally, we show that M-Wnt cells have 1.9-fold increased expression of HIF1α protein compared to metM-Wntlung cells when grown in hypoxia. Conclusions Our results collectively suggest that non-metastatic M-Wnt cells are less sensitive to treatment with 1,25(OH)2D and 2DG in hypoxia than metastatic metM-Wntlungcells. These data may be explained, in part, by elevated expression of HIF1α in M-Wnt cells, which may contribute to their improved survival in hypoxia. Funding Sources National Institute of Health and USDA.

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Cytotoxic effect of fungal-sourced Bassiatin on breast cancer cell lines

Journal of Biotechnology ◽

10.1016/j.jbiotec.2017.06.657 ◽

2017 ◽

Vol 256 ◽

pp. S32

Author(s):

Erkay Ozgor ◽

Handan Sevim ◽

Ozer Aylin Gurpinar ◽

Nevin Keskin

Keyword(s):

Breast Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Cytotoxic Effect ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines

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Identification of a stem-like cell population by exposing metastatic breast cancer cell lines to repetitive cycles of hypoxia and reoxygenation

Breast Cancer Research ◽

10.1186/bcr2773 ◽

2010 ◽

Vol 12 (6) ◽

Cited By ~ 103

Author(s):

Elizabeth Louie ◽

Sara Nik ◽

Juei-suei Chen ◽

Marlies Schmidt ◽

Bo Song ◽

...

Keyword(s):

Breast Cancer ◽

Metastatic Breast Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Cell Population ◽

Metastatic Breast ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Metastatic Breast Cancer Cell

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Cytotoxic Effect of Photoluminescent RE3+ Doped Ca3(PO4)2 Nanorods on Breast Cancer Cell Lines

IRBM ◽

10.1016/j.irbm.2019.05.001 ◽

2019 ◽

Vol 40 (5) ◽

pp. 270-278

Author(s):

V.R. Bheeram ◽

R.R. Malla ◽

S. Kumari ◽

A. Saha ◽

S.B. Mukkamala

Keyword(s):

Breast Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Cytotoxic Effect ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines

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Pyrimidinone Associated Triazole Carboxamides: Synthesis, Characterization, Cytotoxicity and DNA Binding Studies

Current Bioactive Compounds ◽

10.2174/1573407215666190328222350 ◽

2020 ◽

Vol 16 (6) ◽

pp. 911-923

Author(s):

Prakash Bhaskar ◽

Suresha K. Tholappanavara ◽

Bhuvanesh S. Kalal ◽

Vasantha Kumar ◽

Ananda K.C. Siddegowda ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Intrinsic Binding Constant ◽

Binding Studies ◽

Human Embryonic Kidney Cell ◽

Hek 293 ◽

Melanoma Cancer ◽

Dna Binding Studies

Background: Pyrimidinones and its derivatives are present in many anti-cancer agents. It has been reported that these substances were proven to have significant activities against different types of human cancers. The incorporation of [1,2,3]-triazole, a nitrogen-rich unit not only increases the efficacy but also increases the lipophilicity of the drug molecule. As our research was to synthesize newer molecules of effective cytotoxicity, we focused on pyrimidinone and [1,2,3]-triazoles systems, as important scaffolds with the expectation of potential cytotoxic properties. Methods: Novel series of [1,2,3]-triazole carboxamides (5a-j) were synthesized, starting from 3-(2- chloroethyl)-2-methyl-6,7,8,9-tetrahydropyrido[1,2-a]pyrimidin-4-one. The structure of all the synthesized compounds was elucidated based on IR, 1H-NMR, 13C-NMR and LC-MS data. Compounds were focused for their in vitro cytotoxicity against A375 melanoma cancer cell lines, MDA-MB-231 breast cancer cell lines and HEK 293-Human embryonic kidney cell lines using colorimetric MTT assay. The potent compound was evaluated for the DNA binding studies. Results: Most of the Pyrimidinone conjugated [1,2,3]-triazole carboxamides found to be selective towards melanoma cancer cell lines than breast cancer cell lines. Compounds 5d, 5i and 5b were effective against A375 cancer cell lines and are found to be non-toxic against HEK 293-Human embryonic kidney cell lines. The potent compound 5d showed good intrinsic binding constant (Kb) value 3.12 x 103 M-1 in UV based DNA titration. Conclusion: Newly synthesized Pyrimidinone conjugated [1,2,3]-triazole carboxamide derivatives showed the significant cytotoxicity and the potent compound showed good intrinsic binding constant in UV based DNA titration.

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