Cell-based therapies for ischemic cardiomyopathy : investigations of intramyocardial retention and safety of high dose intracoronary delivery of c-kit positive cardiac progenitor cells, and therapeutic utility of a novel population of cardiac mesenchymal stem cells expressing stage-specific embryonic antigen-3 (SSEA-3).

Amniotic fluid is a liquid that fills the amniotic cavity which has defense and nutritional functions in fetal development. Human aterm amniotic fluid can be an ideal alternative as a source of mesenchymal stem cells, originating from the neonate. Preclinical studies of second and third trimester amnion fluid cells confirmed the number of potential donors from this wasted material. In several studies, AF-MSCs express mesenchymal markers such as CD90, CD73 (SH3, SH), CD105 (SH2), CD29, CD166, CD49e, CD58 and CD44 (MHC class I). These cells also express HLA-ABC antigens, CD 34, CD 45 which are hematopoietic markers, and endothelial CD31 markers. There is no expression of CD10, CD11b, CD14, CD34, CD117, EMA and HLA-DR, DP, DQ antigens. Most of AF-MSCs have pluripotent properties which are characterized by the discovery of octamer binding protein 3/4 (Oct-3/4), transcription factors Nanog (Nanog), and stage-specific embryonic antigen 4 (SSEA-4) on RT-PCR examination. From this study, 8 million cells was isolated. These cells will be used for research on pelvic organ prolapse therapy by using AF-MSCs. AF-MSCs isolation totally takes 6 weeks. From 1 flask, 2 million of stem cells was obtained. Keywords: amniotic fluid, AF-MSCs

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Effects of Scaffold Material Used in Cardiovascular Surgery on Mesenchymal Stem Cells and Cardiac Progenitor Cells

The Annals of Thoracic Surgery ◽

10.1016/j.athoracsur.2014.08.071 ◽

2015 ◽

Vol 99 (2) ◽

pp. 605-611 ◽

Cited By ~ 9

Author(s):

Chani Hodonsky ◽

Lakshmi Mundada ◽

Shuyun Wang ◽

Russell Witt ◽

Gary Raff ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Progenitor Cells ◽

Cardiovascular Surgery ◽

Cardiac Progenitor Cells ◽

Scaffold Material

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Activation of cardiac progenitor cells through paracrine effects of mesenchymal stem cells

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2008.06.074 ◽

2008 ◽

Vol 374 (1) ◽

pp. 11-16 ◽

Cited By ~ 88

Author(s):

Chiaki Nakanishi ◽

Masakazu Yamagishi ◽

Kenichi Yamahara ◽

Ikuo Hagino ◽

Hidezo Mori ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Progenitor Cells ◽

Cardiac Progenitor Cells ◽

Paracrine Effects

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ISOLATION OF AMNIOTIC FLUID MESENCHYMAL STEM CELLS (AF-MSCs) OBTAINED FROM CAESAREAN SECTIONS

JOURNAL OBGIN EMAS ◽

10.25077/aogj.2.1.1-9.2018 ◽

2019 ◽

Vol 2 (1) ◽

pp. 1-9

Author(s):

Bobby Indra Utama

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Amniotic Fluid ◽

Pelvic Organ ◽

Rt Pcr ◽

Third Trimester ◽

Amniotic Cavity ◽

Hla Dr ◽

Embryonic Antigen ◽

Stage Specific Embryonic Antigen

Amniotic fluid is a liquid that fills the amniotic cavity which has defense and nutritional functions in fetal development. Human aterm amniotic fluid can be an ideal alternative as a source of mesenchymal stem cells, originating from the neonate. Preclinical studies of second and third trimester amnion fluid cells confirmed the number of potential donors from this wasted material. In several studies, AF-MSCs express mesenchymal markers such as CD90, CD73 (SH3, SH), CD105 (SH2), CD29, CD166, CD49e, CD58 and CD44 (MHC class I). These cells also express HLA-ABC antigens, CD 34, CD 45 which are hematopoietic markers, and endothelial CD31 markers. There is no expression of CD10, CD11b, CD14, CD34, CD117, EMA and HLA-DR, DP, DQ antigens. Most of AF-MSCs have pluripotent properties which are characterized by the discovery of octamer binding protein 3/4 (Oct-3/4), transcription factors Nanog (Nanog), and stage-specific embryonic antigen 4 (SSEA-4) on RT-PCR examination. From this study, 8 million cells was isolated. These cells will be used for research on pelvic organ prolapse therapy by using AF-MSCs. AF-MSCs isolation totally takes 6 weeks. From 1 flask, 2 million of stem cells was obtained.

Download Full-text