Evaluation and Molecular Characterization of Beauveria bassiana for the Control of the Glassy-winged Sharpshooter (Homoptera: Cicadellidae) in California

2008 ◽  
Vol 43 (2) ◽  
pp. 241-246 ◽  
Author(s):  
Surendra K. Dara ◽  
Michael R. McGuire ◽  
Mauricio Ulloa ◽  
Harry K. Kaya
Keyword(s):  
Beauveria Bassiana ◽  
Genetic Relatedness ◽  
Plant Diseases ◽  
Insect Vector ◽  
Homalodisca Coagulata ◽  
Important Pest ◽  
Spissistilus Festinus ◽  
Fungus Beauveria Bassiana ◽  
Almond Leaf Scorch

The glassy-winged sharpshooter, Homalodisca coagulata (Say), is an important pest on grapes, citrus, almonds and other commercial crops in California as it is a vector of Xylella fastidiosa Wells, a bacterium that causes Pierce's disease in grapes, citrus variegated chlorosis, almond leaf scorch and other plant diseases. Various entomopathogenic fungi isolated from natural infections of H. coagulata, its habitats and other insect hosts were evaluated against this insect vector. Based on these studies, 3 isolates of the hyphomycetous fungus, Beauveria bassiana (Balsamo) Vuillemin, were selected for further evaluation. Two of these were California isolates, one each from the three-cornered alfalfa hopper, Spissistilus festinus (Say), and soil from H. coagulata habitat, and the third was a Texas isolate from natural infections of H. coagulata. All three were similar in their virulence to H. coagulata under laboratory conditions. The genetic relatedness of the B. bassiana isolates also was compared using single sequence repeat (SSR) markers which showed genetic diversity of this species based on the source of the isolate. Some isolates were 4× more infectious than others demonstrating that virulence of B. bassiana is not necessarily associated with their genetic relatedness.

Characterization of T-DNA insertion mutants with decreased virulence in the entomopathogenic fungus Beauveria bassiana JEF-007

2016 ◽  
Vol 100 (20) ◽  
pp. 8889-8900 ◽  
Author(s):  
Sihyeon Kim ◽  
Se Jin Lee ◽  
Yu-Shin Nai ◽  
Jeong Seon Yu ◽  
Mi Rong Lee ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungus ◽  
Dna Insertion ◽  
Fungus Beauveria Bassiana ◽  
Insertion Mutants

scholarly journals Peningkatan Kemandirian Kelompok Petani Pengembang Agensia Hayati Dadi Makmur Untuk Memproduksi Aktivator Jamur Entomopatogen Beauveria bassiana Skala Rumah Tangga

2019 ◽  
Vol 3 (2) ◽  
pp. 67
Author(s):  
Siti Nur Aisyah ◽  
Agung Astuti
Keyword(s):  
Focus Group ◽  
Beauveria Bassiana ◽  
Focus Group Discussion ◽  
Group Discussion ◽  
Starter Culture ◽  
Technical Skill ◽  
Training And Supervision ◽  
Laboratory Training ◽  
Fungus Beauveria Bassiana

Kelompok petani pengembang agensia hayati (KPPAH) Dadi Makmur merupakan salah satu kelompok tani di Kabupaten Sleman yang secara aktif menerapkan pengendalian biologis dengan memanfaatkan jamur entomopatogen Beauveria bassiana. Permasalahannya adalah produksi agensia hayati ini terkendala oleh ketersediaan kultur aktivator yang disuplai dari pihak lain. Oleh karena itu, diperlukan adanya kegiatan peningkatan kemandirian dalam bentuk pelatihan dan pendampingan terkait proses pembuatan aktivator B. bassiana menggunakan prosedur yang dapat diaplikasikan pada skala rumah tangga. Tujuan program ini untuk memperkenalkan prosedur produksi aktivator B. bassiana sehingga dapat meningkatkan pemahaman, kemampuan teknis dan kemandirian dari KPPAH ini. Tahapan kegiatannya meliputi pemetaan level pengetahuan dasar melalui aktivitas focus group discussion (FGD), pelatihan laboratorium dan pendampingan produksi aktivator B. bassiana di lokasi mitra. Hasil menunjukkan bahwa peningkatan kemandirian yang sangat signifikan dalam produksi aktivator (inokulum F2 dan F3) (100%), inkubasi inokulum F2 dan F3 (73,4%), dan mengamati karakteristik inokulum yang siap untuk diaplikasikan (75%). Ditinjau dari segi kemampuan teknis, anggota KPPAH Dadi Makmur menunjukkan tingkat kecakapan yang berbeda, terutama dalam kegiatan produksi inokulum F2 dan F3.[Dadi Makmur is one of farmer communities in Sleman Regency that has applied the biological control actively and continously by utilizing the entomopathogenic fungus, Beauveria bassiana. The main problems found are this fungus production was constrained by the limited supply of activator culture from other party. Therefore, an independency improvement activity, in the form of training and supervision, is urgently needed, particularly related to the production of B. bassiana starter culture using an applicable method for home industry. This program was aimed to introduce the procedure used in the production of starter culture, thus it could improve the understanding, technical skill and the independency of this community. This activity consisted of several steps, such as mapping of basic understanding level through focus group discussion, laboratory training and on-site supervision of B. bassiana starter production. The results recorded a significant increase on people’s independency in the preparation of activator culture (F2 and F3) (100%), incubation of activator culture (73.4%) and characterization of a ready-to-apply inoculum (75%). Based on the technical skill, each member performed various levels of performance, particularly during the preparation of starter culture.]

scholarly journals Molecular Characterization of a Novel Mycovirus Infecting the Entomopathogenic Fungus Beauveria Bassiana

2021 ◽  
Author(s):  
Najie Shi ◽  
Fei Hu ◽  
Ping Wang ◽  
Yuxiang Zhang ◽  
Qiuyan Zhu ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungus ◽  
High Throughput Sequencing ◽  
Hypothetical Protein ◽  
Complete Sequence ◽  
The Novel ◽  
Distinct Lineage ◽  
Fungus Beauveria Bassiana ◽  
Infected Sample

Abstract The entomopathogenic fungus Beauveria bassiana is used worldwide for its biological control. Seven dsRNA segments were detected from a single Beauveria bassiana strain RCEF 1446. High-throughput sequencing indicated the presence of three mycoviruses in the infected sample. Two known mycoviruses were identified as Beauveria bassiana Victorivirus 1 and Beauveria bassiana polymycovirus 1, and the novel mycovirus was designated as Beauveria bassiana bipartite mycovirus 1 (BbBV1). The complete sequence of the BbBV1 is described here. The mycovirus contains two dsRNA segments. The first dsRNA is 2,026 bp in length and encoding a RNA-dependent RNA polymerase (RdRp) (68.54 kDa), while the second segment encoding a hypothetical protein (35.55 kDa) of unknown function, was 1,810 bp in length. Moreover, the RdRp protein sequences showed the highest identity of 62.89% to Corynespora cassiicola bipartite mycovirus 1. Phylogenetic analysis of the RdRp reveals that the virus represents a distinct lineage of unassigned dsRNA mycoviruses infecting fungi.

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