Fiber-modified adenovirus-mediated suicide gene therapy can efficiently eliminate bladder cancer cells in vitro and in vivo

AbstractThe ultimate goal of gene delivery vectors is to establish specific and effective treatments for human diseases. We previously demonstrated that human JC polyomavirus (JCPyV) virus-like particles (VLPs) can package and deliver exogenous DNA into susceptible cells for gene expression. For tissue-specific targeting in this study, JCPyV VLPs were conjugated with a specific peptide for bladder cancer (SPB) that specifically binds to bladder cancer cells. The suicide gene thymidine kinase was packaged and delivered by SPB-conjugated VLPs (VLP-SPBs). Expression of the suicide gene was detected only in human bladder cancer cells and not in lung cancer or neuroblastoma cells susceptible to JCPyV VLP infection in vitro and in vivo, demonstrating the target specificity of VLP-SPBs. The gene transduction efficiency of VLP-SPBs was approximately 100 times greater than that of VLPs without the conjugated peptide. JCPyV VLPs can be specifically guided to target particular cell types when tagged with a ligand molecule that binds to a cell surface marker, thereby improving gene therapy.

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926. FK228 Increases Adenovirus Transgene Expression and Enhances Adenovirus Mediated Suicide Gene Therapy in Human Bladder Cancer Cells

Molecular Therapy ◽

10.1016/s1525-0016(16)41368-7 ◽

2003 ◽

Vol 7 (5) ◽

pp. S357

Keyword(s):

Gene Therapy ◽

Bladder Cancer ◽

Cancer Cells ◽

Transgene Expression ◽

Suicide Gene ◽

Suicide Gene Therapy ◽

Human Bladder Cancer ◽

Human Bladder ◽

Bladder Cancer Cells ◽

Human Bladder Cancer Cells

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ENHANCED SENSITIVITY OF BLADDER CANCER CELLS TO CISPLATIN MEDIATED CYTOTOXICITY AND APOPTOSIS IN VITRO AND IN VIVO BY THE SELECTIVE CYCLOOXYGENASE-2 INHIBITOR JTE-522

The Journal of Urology ◽

10.1097/01.ju.0000131945.74377.ad ◽

2004 ◽

Vol 172 (4 Part 1) ◽

pp. 1474-1479 ◽

Cited By ~ 18

Author(s):

YOICHI MIZUTANI ◽

HIROYUKI NAKANISHI ◽

YONG NAN LI ◽

NODOKA SATO ◽

AKIHIRO KAWAUCHI ◽

...

Keyword(s):

Bladder Cancer ◽

Cancer Cells ◽

Cyclooxygenase 2 ◽

Enhanced Sensitivity ◽

Bladder Cancer Cells

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Evaluation of Su Fu’ning Lotion’s Inhibitory Effects on Bladder Cancer Cells In Vitro and In Vivo by Intravesical Instillation

Integrative Cancer Therapies ◽

10.1177/1534735415596569 ◽

2015 ◽

Vol 15 (1) ◽

pp. 80-86 ◽

Cited By ~ 1

Author(s):

Liansheng Ren ◽

Xihua Yang ◽

Lili Zhao ◽

Hong Zhang ◽

Jing Wang

Keyword(s):

Bladder Cancer ◽

Cancer Cells ◽

Intravesical Instillation ◽

Inhibitory Effects ◽

Bladder Cancer Cells

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590. SuperCD Enables Low Dose 5-FC Application for Effective Suicide Gene Therapy in a Rat Hepatoma Model Both In Vitro and In Vivo

Molecular Therapy ◽

10.1016/j.ymthe.2004.06.517 ◽

2004 ◽

Vol 9 ◽

pp. S223

Keyword(s):

Gene Therapy ◽

Low Dose ◽

Suicide Gene ◽

Suicide Gene Therapy ◽

Hepatoma Model ◽

Rat Hepatoma

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DEPDC1B is a tumor promotor in development of bladder cancer through targeting SHC1

Cell Death and Disease ◽

10.1038/s41419-020-03190-6 ◽

2020 ◽

Vol 11 (11) ◽

Author(s):

Chin-Hui Lai ◽

Kexin Xu ◽

Jianhua Zhou ◽

Mingrui Wang ◽

Weiyu Zhang ◽

...

Keyword(s):

Bladder Cancer ◽

Cancer Cells ◽

Malignant Tumors ◽

Tumor Grade ◽

In Vivo Studies ◽

Mechanistic Study ◽

Tumor Promotor ◽

Bladder Cancer Cells

AbstractBladder cancer is one of the most commonly diagnosed malignant tumors in the urinary system and causes a massive cancer-related death. DEPDC1B is a DEP domain-containing protein that has been found to be associated with a variety of human cancers. This study aimed to explore the role and mechanism of DEPDC1B in the development of bladder cancer. The analysis of clinical specimens revealed the upregulated expression of DEPDC1B in bladder cancer, which was positively related to tumor grade. In vitro and in vivo studies showed that DEPDC1B knockdown could inhibit the growth of bladder cancer cells or xenografts in mice. The suppression of bladder cancer by DEPDC1B was executed through inhibiting cell proliferation, cell migration, and promoting cell apoptosis. Moreover, a mechanistic study found that SHC1 may be an important route through which DEPDC1B regulates the development of bladder cancer. Knockdown of SHC1 in DEPDC1B-overexpressed cancer cells could abolish the promotion effects induced by DEPDC1B. In conclusion, DEPDC1B was identified as a key regulator in the development of bladder cancer, which may be used as a potential therapeutic target in the treatment of bladder cancer.

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In vitro and in vivo effects of HAL on porphyrin production in rat bladder cancer cells (AY27)

Journal of Porphyrins and Phthalocyanines ◽

10.1142/s1088424619500615 ◽

2019 ◽

Vol 23 (07n08) ◽

pp. 813-820

Author(s):

Odrun A. Gederaas ◽

Harald Husebye ◽

Anders Johnsson ◽

Susan Callaghan ◽

Anders Brunsvik

Keyword(s):

Bladder Cancer ◽

Cancer Cells ◽

Aminolevulinic Acid ◽

Protoporphyrin Ix ◽

Active Agent ◽

Laser Scanning Microscopy ◽

Rat Bladder ◽

Bladder Cancer Cells

Aminolevulinic acid and hexyl-aminolevulinate serve as biological precursors to produce photosensitive porphyrins in cells via the heme biosynthetic pathway. This pathway is integral to porphyrin-based photodynamic diagnosis and therapy. By adding exogenous hexyl-aminolevulinate to rat bladder cancer cells (AY27, in vitro) and an animal bladder cancer model (in vivo), fluorescent endogenous porphyrin production was stimulated. Lipophilic protoporphyrin IX was identified as the dominant species by reverse high-pressure liquid chromatography. Subcellular porphyrin localization in the AY27 cells was evaluated by confocal laser scanning microscopy and showed almost quantitative bleaching after 20 s. From this study, we ascertained that the protocol described herein is suitable for hexyl-aminolevulinate-mediated photodynamic therapy and diagnosis when protoporphyrin IX is the active agent.

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