Polymorphism of Beta (â) Casein Gene and Their Association with Milk Production Traits in Malvi and Nimari Breeds of Cattle

2019 ◽  
Author(s):  
Akhilesh Pandey ◽  
M. S. Thakur ◽  
Y. Pandey
Keyword(s):  
Restriction Endonuclease ◽  
Milk Production ◽  
Research Work ◽  
Production Traits ◽  
Veterinary Science ◽  
Milk Production Traits ◽  
Casein Gene ◽  
Restriction Sites ◽  
Endonuclease Enzyme

Research work was carried out on 50 Malvi and 50 Nimari breed of cattle in the Department of Animal Genetics and Breeding of College of Veterinary Science and A.H Jabalpur. In present research work the PCR amplified products of 121bp was digested by restriction endonuclease enzyme DdeI, which recognizes G^AATTC sites. Present study showed that the presence of no restriction sites for the enzyme DdeI and only one band of 121bp was observed on the gel and such genotype was designated as A2A2 type. The â- casein gene showed A2A2 genotypes were observed in Malvi and Nimari breeds of cattle. The result of RFLP revealed that the gene and genotypic frequencies of â-casein (CSN2) gene for A2A2 was 1.00 for both Malvi and Nimari breed of cattle. So 100% frequency of A2 allele was observed in both the breeds of cattle under the study.

Polymorphism of beta (â) casein gene and their association with milk production traits in Sahiwal and HF crossbred cattle

2019 ◽  
Author(s):  
Akhilesh Pandey ◽  
M.S Thakur ◽  
Y. Pandey
Keyword(s):  
High Frequency ◽  
Restriction Site ◽  
Research Work ◽  
Production Traits ◽  
Veterinary Science ◽  
Crossbred Cattle ◽  
Milk Production Traits ◽  
Casein Gene ◽  
Restriction Sites ◽  
Endonuclease Enzyme

Present research work was carried out on 50 Sahiwal and 50 HF Crossbred cattle in the Department of Animal Genetics and Breeding of College of Veterinary Science and A H Jabalpur. During the research work the PCR amplified products of 121bp was digested by restriction endonuclease enzyme DdeI, which recognizes G^AATTC sites. The patterns evolved in the present study showed that the presence of one restriction site on one alleles and absence of restriction site on other alleles resulted in the appearance of three bands of 121, 86 and 35bp.This genotype was referred to as A1A2. When both strand had no restriction sites for the enzyme, only one band of 121bp was observed on the gel such genotype was designated as A2A2 type. The â- casein gene showed A2A2 and A1A2 genotypes were observed in Sahiwal and HF crossbred cattle. The genotypic frequencies of â-casein (CSN2)/ DdeI gene for A1A1, A1A2 and A2A2 are 0.00, 0.30, and 0.70 in Sahiwal and 0.00, 0.64 and 0.36 in HF crossbred cattle, respectively and the gene frequency A1 and A2 is 0.15 and 0.85 in Sahiwal and 0.32 and 0.68 in HF crossbred cattle. High frequency of A2 allele was observed in both the breeds of cattle under the study.

Association Study of Polymorphic Varients of Beta (B) Casein Gene with Milk Production Traits (Lactose, Snf and Density) in Malvi, Nimari, Sahiwal and H.F. Cross Breeds Cow

2020 ◽  
Author(s):  
Akhilesh Pandey ◽  
M.S. Thakur ◽  
Y. Pandey
Keyword(s):  
Association Study ◽  
Bos Taurus ◽  
Research Work ◽  
Production Traits ◽  
Crossbred Cattle ◽  
Restriction Sites ◽  
Beta Casein ◽  
Polymorphic Variants ◽  
European Cattle ◽  
Endonuclease Enzyme

Background: The most frequently observed forms of beta casein (β-Cn) in dairy cattle are A1 and A2. A2 β-Cn is recognized as the original β-Cn protein because it existed before a mutation caused the appearance of A1 β-Cn in European cattle (Bos taurus) a few thousand years ago. In cattle, beta-casein (CSN2) gene is highly polymorphic with at least 13 genetic variants known until now.Methods: Research work was carried out on 50 Malvi and 50 Nimari, 50 Sahiwal and 50 H F Cross bred cow. In present research work the PCR amplicons of 121bp were digested by restriction endonuclease enzyme DdeI, which recognizes G^AATTC sites. Present association study of polymorphic variants’ showed that the presence of no restriction sites for the enzyme DdeI in both Malvi and Nimari. So one band of 121bp was observed on the gel and such genotype was designated as A2A2 type. Whereas in Sahiwal and H F Crossbred showed two type of genotypes A1A2 and A2A2.Result: The result of RFLP revealed that the gene and genotypic frequencies of β-casein (CSN2) gene for A2A2 was 1.00 for both Malvi and Nimari breed of cattle but 0.00, 0.30 and 0.70 in Sahiwal and 0.00, 0.64 and 0.36 in HF crossbred cattle, respectively. Association study showed that the lactose per cent was significantly higher in Nimari as compared to Malvi and Sahiwal. Among all the four breeds of cattle, significantly lower SNF (%) and density was noticed in Malvi breed of cattle for A2A2 genotype compared to remaining three breeds.

scholarly journals Association of bGH and Pit-1 gene variants with milk production traits in dairy Gyr bulls

2004 ◽  
Vol 39 (2) ◽  
pp. 147-150 ◽  
Author(s):  
Keiko Kusamura de Mattos ◽  
Silvia Nassif Del Lama ◽  
Mario Luiz Martinez ◽  
Ary Ferreira Freitas
Keyword(s):  
Dairy Cattle ◽  
Milk Production ◽  
Gene Polymorphisms ◽  
Restriction Site ◽  
Gene Variants ◽  
Production Traits ◽  
Milk Production Traits ◽  
Restriction Sites ◽  
Gene Restriction ◽  
Marker Information

The objective of this study was to obtain genetic marker information in the Gyr breed by analyzing bGH and Pit-1 gene polymorphisms and to verify their association with milk production traits. One sample including 40 Gyr bulls were genotyped at two bGH gene restriction sites (bGH- AluI and bGH-MspI) and at one restriction site in the Pit-1 gene (Pit-1 HinfI). The bGH-MspI(-) allele was favorable for fat milk percentage. The heterozigous Pit-1 HinfI (+/-) bulls were superior for fat milk production, in relation to homozigous Pit-1 HinfI (+/+). The Pit-1 and bGH genes are strong candidates in the dairy cattle QTL search, and zebuine populations are promising samples for this purpose.

Effect of polymorphisms in the CSN3 (κ-casein) gene on milk production traits in Chinese Holstein Cattle

2014 ◽  
Vol 41 (11) ◽  
pp. 7585-7593 ◽  
Author(s):  
M. A. Alim ◽  
T. Dong ◽  
Y. Xie ◽  
X. P. Wu ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Milk Production ◽  
Holstein Cattle ◽  
Production Traits ◽  
Milk Production Traits ◽  
Casein Gene ◽  
Chinese Holstein ◽  
Chinese Holstein Cattle

scholarly journals Amplification created restriction sites for genotyping SNPs in the bovine ABCG2 and its association with milk production traits (Brief Report)

2009 ◽  
Vol 52 (6) ◽  
pp. 647-649
Author(s):  
I. Kowalewska-Łuczak ◽  
H. Kulig ◽  
M. Kmieć
Keyword(s):  
Milk Production ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Production Traits ◽  
Milk Production Traits ◽  
Pcr Rflp ◽  
Restriction Sites ◽  
Mouse Milk ◽  
Jersey Cows ◽  
Abcg2 Gene

Abstract. ABCG2 (ATP-binding cassette, subfamily G, member 2) belongs to the superfamily of ATPbinding cassette (ABC) transporters. In ATP-dependent processes, ABCG2 is responsible for transporting xenobiotics and cytostatic drugs across various cellular membranes. The ABCG2 gene is expressed in the apical membrane of alveolar mammary epithelial cells and is responsible for the active secretion of substrates into mouse milk. Other members of the ABC subfamily G are sterol transporters. It therefore appears that ABCG2 might transport cholesterol into milk (COHEN-ZINDER et al. 2005). In the study by COHEN-ZINDER et al. (2005), several SNPs were detected in the ABCG2 gene but only two were genotyped – in exon 14 and in intron 3. In the case of SNP A/C in exon 14 resulting in an amino acid change Y581S, it was demonstrated that this substitution affects milk yield and composition. To detect theses polymorphisms in our study we used a new PCR-RFLP method based on an amplification created restriction site (ACRS). This method has been frequently used by various researchers in recent times (e.g. ZYCH et al. 2007). The aim of this study was to estimate the frequencies of genotypes and alleles and to investigate possible associations between ABCG2 polymorphisms and milk production traits in Jersey cows.

áS2 casein gene polymorphism and association with milk production traits in Malvi and Nimari cattle of Madhya Pradesh

2018 ◽  
Author(s):  
Akhilesh Pandey and M.S.Thakur
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Madhya Pradesh ◽  
Production Traits ◽  
Milk Production Traits ◽  
Casein Gene ◽  
Significant Difference ◽  
Polymorphic Variants ◽  
Daily Milk Yield ◽  
Daily Milk

The aim of this study was to know the polymorphic variants and their association with milk production traits in Malvi and Nimari cattle of Madhya Pradesh at áS2 casein gene (CSN1S2) gene locus. The PCR amplified products of 1267 base pair (bp) length were digested by restriction endonuclease enzyme EcoRV, which recognizes GAT^ATC sites. The 1267bp product was cut into two fragments of sizes 1150bp and 117bp. All the tested samples yielded different results. Absence of restriction site at both the alleles that resulted in the appearance of single compact bands of size 1267bp was referred to as genotype AA. The samples exhibiting three fragments (1267bp/ 1150bp/ 117bp) were denoted as genotype AB. The RFLP analysis carried out in above both of the breeds of cattle revealed dissimilar genotypic patterns. AA and AB genotypes were observed in Malvi and Nimari . The genotypic and gene frequencies of AA, AB and BB genotypes of áS2-casein gene (CSN1S2)/EcoRV locus was found to be 0.44, 0.56 and 0.00 in Malvi; 0.68, 0.32 and 0.00 in Nimari and the respective gene frequency for A and B alleles were found to be 0.72 and 0.28 in Malvi; 0.84 and 0.16 in Nimari. The frequency of A allele was found to be highest as compared to B allele in all the four breeds of cattle under the study. Chi-square values for testing correspondence between observed and expected genotypic frequencies at this locus were found to be non-significant in Malvi and Nimari breeds of cattle. The above result indicated that the populations of animals of above both breeds were in Hardy-Weinberg equilibrium at this locus. The association of polymorphic variants of áS2-casein gene (CSN1S2)/EcoRV in Malvi and Nimari breeds of cattle with milk yield per lactation (L), daily milk yield (L) showed the non significant difference was observed between mean MY(L) of AA and AB genotype of Malvi and Nimari and With respect of daily milk yield the Nimari. However, the Nimari breed showed significantly higher daily milk yield than the Malvi breed of cattle.

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