Association Study of Polymorphic Varients of Beta (B) Casein Gene with Milk Production Traits (Lactose, Snf and Density) in Malvi, Nimari, Sahiwal and H.F. Cross Breeds Cow

Author(s):  
Akhilesh Pandey ◽  
M.S. Thakur ◽  
Y. Pandey

Background: The most frequently observed forms of beta casein (β-Cn) in dairy cattle are A1 and A2. A2 β-Cn is recognized as the original β-Cn protein because it existed before a mutation caused the appearance of A1 β-Cn in European cattle (Bos taurus) a few thousand years ago. In cattle, beta-casein (CSN2) gene is highly polymorphic with at least 13 genetic variants known until now.Methods: Research work was carried out on 50 Malvi and 50 Nimari, 50 Sahiwal and 50 H F Cross bred cow. In present research work the PCR amplicons of 121bp were digested by restriction endonuclease enzyme DdeI, which recognizes G^AATTC sites. Present association study of polymorphic variants’ showed that the presence of no restriction sites for the enzyme DdeI in both Malvi and Nimari. So one band of 121bp was observed on the gel and such genotype was designated as A2A2 type. Whereas in Sahiwal and H F Crossbred showed two type of genotypes A1A2 and A2A2.Result: The result of RFLP revealed that the gene and genotypic frequencies of β-casein (CSN2) gene for A2A2 was 1.00 for both Malvi and Nimari breed of cattle but 0.00, 0.30 and 0.70 in Sahiwal and 0.00, 0.64 and 0.36 in HF crossbred cattle, respectively. Association study showed that the lactose per cent was significantly higher in Nimari as compared to Malvi and Sahiwal. Among all the four breeds of cattle, significantly lower SNF (%) and density was noticed in Malvi breed of cattle for A2A2 genotype compared to remaining three breeds.

Author(s):  
Akhilesh Pandey ◽  
M.S Thakur ◽  
Y. Pandey

Present research work was carried out on 50 Sahiwal and 50 HF Crossbred cattle in the Department of Animal Genetics and Breeding of College of Veterinary Science and A H Jabalpur. During the research work the PCR amplified products of 121bp was digested by restriction endonuclease enzyme DdeI, which recognizes G^AATTC sites. The patterns evolved in the present study showed that the presence of one restriction site on one alleles and absence of restriction site on other alleles resulted in the appearance of three bands of 121, 86 and 35bp.This genotype was referred to as A1A2. When both strand had no restriction sites for the enzyme, only one band of 121bp was observed on the gel such genotype was designated as A2A2 type. The â- casein gene showed A2A2 and A1A2 genotypes were observed in Sahiwal and HF crossbred cattle. The genotypic frequencies of â-casein (CSN2)/ DdeI gene for A1A1, A1A2 and A2A2 are 0.00, 0.30, and 0.70 in Sahiwal and 0.00, 0.64 and 0.36 in HF crossbred cattle, respectively and the gene frequency A1 and A2 is 0.15 and 0.85 in Sahiwal and 0.32 and 0.68 in HF crossbred cattle. High frequency of A2 allele was observed in both the breeds of cattle under the study.


Author(s):  
Akhilesh Pandey ◽  
M. S. Thakur ◽  
Y. Pandey

Research work was carried out on 50 Malvi and 50 Nimari breed of cattle in the Department of Animal Genetics and Breeding of College of Veterinary Science and A.H Jabalpur. In present research work the PCR amplified products of 121bp was digested by restriction endonuclease enzyme DdeI, which recognizes G^AATTC sites. Present study showed that the presence of no restriction sites for the enzyme DdeI and only one band of 121bp was observed on the gel and such genotype was designated as A2A2 type. The â- casein gene showed A2A2 genotypes were observed in Malvi and Nimari breeds of cattle. The result of RFLP revealed that the gene and genotypic frequencies of â-casein (CSN2) gene for A2A2 was 1.00 for both Malvi and Nimari breed of cattle. So 100% frequency of A2 allele was observed in both the breeds of cattle under the study.


2021 ◽  
Vol 245 ◽  
pp. 104403
Author(s):  
Pamela I. Otto ◽  
Simone E.F. Guimarães ◽  
Lucas L. Verardo ◽  
Ana Luísa S. Azevedo ◽  
Claudia A. Sevillano ◽  
...  

2017 ◽  
Vol 57 (10) ◽  
pp. 2096 ◽  
Author(s):  
T. J. Schatz

This study compares the performance of F1 Senepol × Brahman steers (F1 SEN) to Brahman (BRAH) steers in an Indonesian feedlot. The focus was to address concerns that crossbred cattle are discriminated against by live export cattle buyers due to a perception that they do not perform as well as Brahmans in Indonesian feedlots. F1 SEN (n = 54) and BRAH (n = 32) steers that had grazed together since weaning at Douglas Daly Research Farm (Northern Territory) were exported to Indonesia and fed for 121 days in a feedlot near Lampung (Sumatra, Indonesia). The average daily gain of the F1 SEN steers over the feeding period was 0.17 kg/day higher (P < 0.001) than the BRAH steers (1.71 vs 1.54 kg/day). As a result the F1 SEN put on an average of 21.6 kg more over the 121-day feeding period and they did not have a higher mortality rate. Consequently, F1 SEN steers performed better than BRAH in an Indonesian feedlot and these results should encourage live export cattle buyers to purchase this type of cattle (Brahman crossed with a tropically adapted Bos taurus breed) with confidence that they can perform at least as well as Brahmans in Indonesian feedlots, although it should be noted that growth rates are usually higher in F1 crosses than in subsequent generations.


PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0140615 ◽  
Author(s):  
Jingwei Yuan ◽  
Congjiao Sun ◽  
Taocun Dou ◽  
Guoqiang Yi ◽  
LuJiang Qu ◽  
...  

2004 ◽  
Vol 4 (4) ◽  
pp. 452-455 ◽  
Author(s):  
Ali Reza Ahmed . ◽  
Sarder Safiqul Islam . ◽  
Nargis Khanam . ◽  
Ayesha Ashraf .

2020 ◽  
Vol 68 (32) ◽  
pp. 8732-8743
Author(s):  
E. Gómez ◽  
P. Salvetti ◽  
J. Gatien ◽  
M. Muñoz ◽  
D. Martín-González ◽  
...  

2016 ◽  
Vol 56 (10) ◽  
pp. 1643 ◽  
Author(s):  
L. F. Prados ◽  
S. C. Valadares Filho ◽  
S. A. Santos ◽  
D. Zanetti ◽  
A. N. Nunes ◽  
...  

Forty 3/4 Bos indicus × 1/4 Bos taurus bulls (initial bodyweight = 214 ± 4 kg; 11 ± 0.2 months) were used in the study. Four bulls were slaughtered as reference and the other 36 bulls were allowed ad libitum consumption of treatment diets following a completely randomised 3 × 3 factorial design. The design included three diet concentrations of calcium (Ca) and phosphorus (P): standard, 50% of standard, and 75% of standard and three periods of feedlot confinement: 8, 16, and 24 weeks with four bulls per treatment combination. The diets were isonitrogenous and consisted of 60 : 40 corn silage to concentrate. Dry matter intake, crude protein, ether extract, organic matter, non-fibrous carbohydrates, Ca, and P, expressed as kg/day, were greater (P < 0.05) for bulls confined for longer periods. Nutrient digestibility and average daily gain was not affected (P > 0.05) by Ca and P concentration of diet or by feedlot phase. Dressing were decreased (P < 0.05) for bulls that remained in confinement for a shorter period. The daily maintenance requirements of Ca and P were 13.66 and 21.51 mg/kg empty bodyweight, respectively, and the absorption coefficients of Ca and P were 0.70 and 0.67, respectively. We concluded that for crossbred cattle in the feedlot, dietary levels of Ca and P recommended by BR-CORTE (2010), NRC (2000), and AFRC (1991) could be reduced by 62%, 66%, and 57%, respectively, for Ca and by 14%, 15%, and 43%, respectively, for P with significant savings in costs. This study shows that Ca and P can be reduced to 1.8 and 2.2 g/kg DM respectively, in the diets for crossbred cattle in feedlot.


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