Effect of Growth Hormone-releasing Hormone Gene Polymorphism (GHRH/HAEIII) on First Lactation Milk Yield and Milk Composition Traits in Sahiwal Cattle

2021 ◽  
Author(s):  
Manvendra Singh ◽  
Ashwani Arya ◽  
Arun Pratap Singh ◽  
A.K. Gupta ◽  
Sonam Dixit
Keyword(s):  
Growth Hormone ◽  
Milk Yield ◽  
Rflp Analysis ◽  
Milk Composition ◽  
Growth Hormone Releasing Hormone ◽  
Fat Percentage ◽  
Breeding Values ◽  
Releasing Hormone ◽  
Pcr Rflp ◽  
Sahiwal Cattle

Background: Growth hormone-releasing hormone (GHRH), also known as somatoliberin is a releasing hormone of growth hormone (GH). GHRH is a peptide hormone found mainly in the hypothalamus. The gene is significantly associated with increase milk yield in cattle. The objective of this study was to identify the polymorphism in the GHRH gene (GHRH/HaeIII) and to evaluate the relationship between the polymorphism with the breeding values for first lactation milk production and composition traits of Sahiwal cattle reared using PCR-RFLP. Methods: A total of 130 animals were included in the study. PCR-RFLP analysis of each 297bp PCR product was carried out using HaeIII restriction enzyme. The single trait animal model was considered for the estimation of breeding values using WOMBAT software. To study the effect of genotype on the individual trait, PROC GLM (SAS 4.3) was used.Result: PCR-RFLP analysis of GHRH gene revealed three genotypes with genotypic frequencies as; 0.05 (AA), 0.42 (AB) and 0.53 (BB). The allelic frequencies for A and B allele were 0.26 and 0.74, respectively. Sahiwal cows with AA genotype have significantly higher (p less than 0.017) fat percentage in milk while the cows with BB genotype; while the BB genotype cows characterized by significantly higher (p less than 0.025) first lactation 305 day milk yield. Among the three genotypes, BB was most abundant while AA genotype was rarest in the screened Sahiwal samples. The identified potential genetic marker could be used for the development of Marker Assisted Selection (MAS) strategy for higher milk yield and milk composition traits in Sahiwal cattle.

Identification of Genetic Variants in ABCG2 Gene Influencing Milk Production Traits in Dairy Cattle

2019 ◽  
Author(s):  
Arun Pratap Singh ◽  
A K Chakravarty ◽  
M A Mir ◽  
Ashwani Arya ◽  
Manvendra Singh
Keyword(s):  
Milk Yield ◽  
Rflp Analysis ◽  
Milk Composition ◽  
Breeding Value ◽  
Production Traits ◽  
Crossbred Cattle ◽  
Fat Percentage ◽  
Pcr Rflp ◽  
Karan Fries ◽  
Abcg2 Gene

This study was performed to investigate the polymorphisms in the ABCG2 (ATP-binding cassette sub-family G member 2) gene and to reveal the association of genotypes with breeding value (BV) for first lactation milk yield and milk composition traits of Karan Fries (HF crossbred) cattle. The traits were adjusted against the significant effect of non-genetic factors. PCR-RFLP analysis of ABCG2 (exon 14) gene revealed three genotypes. AA genotype in ABCG2 gene had significant effect on BV for average test day fat percentage. The identified potential genetic marker could be used for the development of Marker Assisted Selection (MAS) strategy for higher milk yield and milk composition traits in Karan Fries Cattle.

scholarly journals Associations between polymorphisms of growth hormone releasing hormone (<i>GHRH</i>) and pituitary transcription factor 1 (<i>PIT1</i>) genes and production traits of Limousine cattle

2003 ◽  
Vol 46 (6) ◽  
pp. 527-534 ◽  
Author(s):  
A. Dybus ◽  
M. Kmieć ◽  
Z. Sobek ◽  
W. Pietrzyk ◽  
B. Wiśniewski
Keyword(s):  
Growth Hormone ◽  
Transcription Factor ◽  
Bovine Growth Hormone ◽  
Growth Hormone Releasing Hormone ◽  
Production Traits ◽  
Releasing Hormone ◽  
Pcr Rflp ◽  
Transcription Factor 1

Abstract. Associations between polymorphism of the bovine growth hormone releasing hormone (GHRH) and pituitary transcription factor 1 (PIT1) genes and production traits of Limousine cattle were analysed. A total of 130 calves were included in the study. PCR-RFLP method was used for genotyping. The frequencies of genotypes and alleles of PIT1 and GHRH were as follows: 0.0692 – AA, 0.4077 – AB, 0.5231 – BB, and 0.2731 for PIT1A, 0.7269 for PIT1B; 0.0154 – AA, 0.1692 – AB, 0.8154 – BB, and 0.1 for GHRHA, 0.9 for GHRHB. Associations between polymorphism and production traits of Limousine calves were found. Statistically significant differences (P ≤ 0.01) between individuals of different GHRH genotypes were found in relation to height at sacrum (cm) and height at withers (cm) at 210th day of age. The calves with AA genotype of GHRH were shorter (−8,14 and −8,33 cm) than AB and BB individuals (P ≤ 0.01). The small number of calves with the AA genotype did not enable important conclusions.

scholarly journals Growth Hormone Response to Growth Hormone Releasing Hormone in Calves that Differ in Genetic Merit for Milk Yield

2005 ◽  
Vol 88 (5) ◽  
pp. 1723-1731 ◽  
Author(s):  
W.J. Weber ◽  
L.H. Baumgard ◽  
G.W. Kazmer ◽  
S.A. Zinn ◽  
L.B. Hansen ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Milk Yield ◽  
Growth Hormone Response ◽  
Growth Hormone Releasing Hormone ◽  
Hormone Response ◽  
Releasing Hormone ◽  
Genetic Merit

Association of pituitary adenoma with neuronal christoma: A TEM study

1994 ◽  
Vol 52 ◽  
pp. 232-233
Author(s):  
Eva Horvath ◽  
Kalman Kovacs ◽  
B. W. Scheithauer ◽  
R. V. Lloyd ◽  
H. S. Smyth
Keyword(s):  
Growth Hormone ◽  
Pituitary Adenoma ◽  
Pituitary Tumor ◽  
Nervous Tissue ◽  
Alternative Explanation ◽  
Growth Hormone Releasing Hormone ◽  
Cell Hyperplasia ◽  
Releasing Hormone ◽  
Secretory Neurons

The association of a pituitary adenoma with nervous tissue consisting of neuron-like cells and neuropil is a rare abnormality. In the majority of cases, the pituitary tumor is a chromophobic adenoma, accompanied by acromegaly. Histology reveals widely variable proportions of endocrine and nervous tissue in alternating or intermingled patterns. The lesion is perceived as a composite one consisting of two histogenetically distinct parts. It has been suggested that the neuronal component, morphologically similar to secretory neurons of the hypothalamus, may initiate adenoma formation by releasing stimulatory substances. Immunoreactivity for growth hormone releasing hormone (GRH) in the neuronal component of some cases supported this view, whereas other findings such as consistent lack of growth hormone (GH) cell hyperplasia in the lesions called for alternative explanation.Fifteen tumors consisting of a pituitary adenoma and a neuronal component have been collected over a 20 yr. period. Acromegaly was present in 11 patients, was equivocal in one, and absent in 3.

Treatment with growth hormone-releasing hormone (GHRH) 1-44 stimulates growth velocity in growth hormone-deficient children

1989 ◽  
Vol 120 (3_Suppl) ◽  
pp. S85-S86
Author(s):  
Y.-F. SHI ◽  
X.-L. BAO ◽  
R. Liu ◽  
J.-Y. DENG ◽  
W. REINHARDT ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Velocity ◽  
Growth Hormone Releasing Hormone ◽  
Releasing Hormone
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