In vitro plant regeneration in pigeonpea [Cajanus cajan (L.) MILL Sp.] using various explants

2016 ◽  
Author(s):  
K. Nandha Abhijeeta ◽  
B. B Madariya Rajesh
Keyword(s):  
Plant Regeneration ◽  
Efficient Method ◽  
Cajanus Cajan ◽  
Callus Formation ◽  
Ms Medium ◽  
In Vitro Plant Regeneration ◽  
In Vitro Plant

An efficient method for plant regeneration via callus formation has been developed in pigeonpea using two varieties as BDN-2 and GT-101. Three different explants viz. leaf, hypocotyl and root excised from 15-20 days old in vitro raised seedlings were cultured on basal MS medium. Greenish coloured callus were observed using various media according to explants. More shoots were found in MS + 1.0 mg/l NAA + 4.0 mg/l Adenine and roots on Elongated shoots in MS + 1.0 mg/l IAA media. Rooted plants were transferred in pot for acclimatization.

scholarly journals In vitro plant regeneration of Iraqi cotton (Gossypium hirsutum L.) cultivars through embryonic axis

2014 ◽  
Vol 8 (2) ◽  
pp. 90-94
Author(s):  
Hussein Abdullah Ahmed ◽  
Mortaza Hajyzadeh ◽  
Surendra Barpete ◽  
Sebahattin Ozcan
Keyword(s):  
Plant Regeneration ◽  
Gossypium Hirsutum ◽  
Activated Charcoal ◽  
Embryonic Axis ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
In Vitro Plant Regeneration ◽  
Gossypium Hirsutum L ◽  
In Vitro Plant

In plant tissue culture studies, obtaining new plantlets from different parts of plant is a very important feature with direct or indirect ways of regeneration. The plant show different regeneration capacities from species to species. In this present study, in-vitro direct shoots development from embryonic axis of cotton (Gossypium hirsutum L. cv. Lashata) were comparatively studied. Embryonic axis, embryonic hypocotyl and plumule obtained from five days old in-vitro grown seeds. All explants were cultured on MS media supplemented with 1- 4 mg/l Benzyl amino purine (BAP) + 0.1 mg/l Naphthalene acetic acid (NAA) and 3 mg/l activated charcoal. Cultured explants were kept at growth chamber with photoperiod for 16 hours light and 8 hours dark, at 25oC. Reproducible in-vitro plant regeneration was obtained from embryonic axis when cultured onto MS medium supplemented 1 mg/l BAP + 0.1 mg/l NAA and 3 g/l Activated charcoal. Shoot induction was to be noted 53.3%, 36.7% and 12.3 in embryonic axis, plumule and embryonic hypocotyl respectively. All these shoots were capable of rooting on MS medium supplemented with 0.1 mg/l NAA, and establishing in soil 3-4 weeks.

scholarly journals An Efficient Method for in vitro Plant Regeneration and Micropropagation of Aloe saponaria

2015 ◽  
Vol 12 (Special-Edn2) ◽  
pp. 01-05
Author(s):  
Sook Young Lee ◽  
T. Bastin Baskar ◽  
Haeng Hoon Kim ◽  
Jai-Heon Lee ◽  
Sang Un Park
Keyword(s):  
Plant Regeneration ◽  
Efficient Method ◽  
In Vitro Plant Regeneration ◽  
In Vitro Plant
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