In-Silico Investigation of Betasatellite Complexity in Papaya Leaf Curl Disease Complex

2018 ◽  
Vol 4 (02) ◽  
pp. 41-49
Author(s):  
Saurabh Verma ◽  
Sangeeta Saxena
Keyword(s):  
Molecular Diversity ◽  
Evolutionary Relationship ◽  
Monopartite Begomoviruses ◽  
Multiple Sequence ◽  
Intraspecific Interaction ◽  
Disease Complex ◽  
Genetic Complexity ◽  
Relationship Of ◽  
Leaf Curl Disease ◽  
Leaf Curl

Papaya leaf curl disease complex (PLCD) impose heavy losses to papaya farmers across India. It is mainly reported to be caused by Papaya leaf curl virus and associated begomoviruses carrying a DNA-A and betasatellite molecules. Both components are required for infliction of severe symptoms. The investigation into molecular diversity is necessary to devise intervention techniques against these begomoviruses. Based on the outcome of diversity, preferred resistance strategy against a disease complex should be generic in nature, which provides an advantage of a broad range resistance against a variety of plant virus causing same disease symptoms. For this purpose, we studied the molecular diversity through investigation of genetic complexity of the betasatellite genome using various computational methods and probed genetic complexity of betasatellite component. Multiple sequence alignment and recombination analysis were performed to understand the evolutionary relationship of betasatellite components with a DNA-A present as a helper genome in case of monopartite begomoviruses. The overall results indicate that the betasatellite molecules have evolved independently of DNA-A component and their association is a result of co-infection and due to inter- and intraspecific interaction with various forms of virus infection in plants. The information generated through this study has potential application in designing intervention strategies against PLCD, which is widespread in Indian sub-continent region that includes countries like India, Pakistan, Nepal and Bangladesh.

Cotton leaf curl disease is associated with multiple monopartite begomoviruses supported by single DNA ?

2003 ◽  
Vol 148 (10) ◽  
pp. 1969-1986 ◽  
Author(s):  
S. Mansoor ◽  
R. W. Briddon ◽  
S.E. Bull ◽  
I. D. Bedford ◽  
A. Bashir ◽  
...  
Keyword(s):  
Monopartite Begomoviruses ◽  
Cotton Leaf ◽  
Cotton Leaf Curl Disease ◽  
Leaf Curl Disease ◽  
Leaf Curl

scholarly journals Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso

2010 ◽  
Vol 7 (1) ◽  
Author(s):  
Fidèle Tiendrébéogo ◽  
Pierre Lefeuvre ◽  
Murielle Hoareau ◽  
Julie Villemot ◽  
Gnissa Konaté ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Molecular Diversity ◽  
Cotton Leaf ◽  
Satellite Dnas ◽  
Leaf Curl Disease ◽  
Virus Isolates ◽  
Leaf Curl

scholarly journals Identification of Chili leaf curl virus Causing Leaf Curl Disease of Petunia in Oman

Plant Disease ◽  
2014 ◽  
Vol 98 (4) ◽  
pp. 572-572 ◽  
Author(s):  
A. A. Al-Shihi ◽  
S. Akhtar ◽  
A. J. Khan
Keyword(s):  
Indian Subcontinent ◽  
Rolling Circle Amplification ◽  
Rapid Evolution ◽  
Post Inoculation ◽  
Multiple Sequence ◽  
Rolling Circle ◽  
Begomovirus Genome ◽  
Leaf Curl Virus ◽  
Leaf Curl Disease ◽  
Leaf Curl

Petunias (Petunia × hybrida) are the most important ornamental plants in Oman. In 2012, petunias were observed in public parks and airport landscape in Dhofar region with symptoms of upward leaf curling, yellowing and vein clearing, and size reduction in leaves. Almost all plants in the surveyed landscape showed high infestation of Bemisia tabaci and symptoms that suggested infection with a begomovirus. Six symptomatic samples were collected from three different sites. All symptomatic samples were found PCR-positive with diagnostic primers for begomovirus (3) when DNA extracted from infected leaves was used as template. Nucleic acids extracted from the symptomatic leaves were used to amplify circular DNA molecules by rolling circle amplification method. The amplified concatameric products were digested with restriction enzyme PstI, which yielded a product ∼2.8 kb in size. The putative begomovirus fragment was cloned and sequenced in both orientations. Partial sequences of six clones were 99 to 100% similar and thus only two clones, PT-2 and PT-3, were fully sequenced. The whole genomes of both clones were 2,761 bp, and both were deposited in GenBank under accession numbers HF968755 and HF968756 for the isolates PT-2 and PT-3, respectively. Both sequences had six open reading frames; Rep, TrAP, REn, and C4 genes in complementary sense; and CP and V2 genes in virion-sense, typical of the begomovirus genome organization. Upon alignment, the two sequences showed 99.4% nucleotide identity with each other, thus representing isolates of a single begomovirus species. BlastN comparison showed PT-2 and PT-3 from petunia were 94 to 95% identical to the sequences of ChCLV from Oman (JN604490 to JN604500), which were obtained from other hosts. ClustalV multiple sequence alignment showed that isolates PT-2 and PT-3 shared maximum sequence identity of 93.3 and 92.8%, respectively, with an isolate of ChLCV-OM (JN604495). According to ICTV rules for begomoviruses, PT-3 should be considered to be a new strain of ChLCV-OM and PT-2 a variant of the already existing ChLCV-OM strain. We propose the name for this new strain as the “Petunia strain” of Chili leaf curl virus (ChLCV-Pet). Two infectious clones were constructed from the PT-2 and PT-3 sequences, clones as 1.75-genome sequences in a binary vector, suitable for agroinfection to confirm their infectivity. Both clones, PT-2 and PT-3, produced typical leaf curl disease symptoms upon inoculation on petunia 18 days post inoculation. The presence of the same virus in symptomatic field infected and inoculated petunia was confirmed by Southern blot using 650 bp DIG labeled probe prepared from CP region of PT-3 isolate. ChLCV-OM, a monopartite begomovirus, is widely associated with leaf curl disease of tomato and pepper in Oman, with its origin traced to the Indian subcontinent (2). Identification of a new strain of ChLCV from petunia provides evidence of an ongoing rapid evolution of begomoviruses in this region. Although petunia has been tested as an experimental host for some begomoviruses (1,4), this is the first report of petunia as natural host for ChLCV, a begomovirus previously reported in tomato and pepper in Oman. References: (1) Cui et al. J. Virol. 78:13966, 2004. (2) Khan et al. Virus Res. 177:87, 2013. (3) Khan et al. Plant Dis. 97:1396, 2013. (4) Urbino et al. Arch. Virol. 149:417, 2003.

scholarly journals Cotton leaf curl disease complex (leaf curl disease of cotton).

2021 ◽  
Author(s):  
Judith K. Brown
Keyword(s):  
South Asia ◽  
Ornamental Plants ◽  
Seed Transmission ◽  
Growing Season ◽  
The Philippines ◽  
Cotton Leaf ◽  
Disease Complex ◽  
Cotton Leaf Curl Disease ◽  
Leaf Curl Disease ◽  
Leaf Curl

Abstract Leaf curl disease of cotton caused by the CLCuD-complex of begomoviruses is endemic to Pakistan and India and perhaps other nearby locales in south Asia. It has been introduced from there to China and the Philippines on ornamental plants, from where it has spread to infect cotton and okra in China. Losses are difficult to assess, but early estimates (pre-1990) range up to 20% when infection occurs early in the growing season and/or with highly susceptible cultivars Viruliferous whiteflies on infested/infected plants harbouring CLCuD-begomoviruses imported to other cotton-growing countries, in particular, are of concern in preventing introduction under optimal circumstances. No seed transmission is known to occur.

Further changes in the cotton leaf curl disease complex: an indication of things to come?

Virus Genes ◽  
2017 ◽  
Vol 53 (6) ◽  
pp. 759-761 ◽  
Author(s):  
Ishtiaq Hassan ◽  
Imran Amin ◽  
Shahid Mansoor ◽  
Rob W. Briddon
Keyword(s):  
Cotton Leaf ◽  
Disease Complex ◽  
Cotton Leaf Curl Disease ◽  
To Come ◽  
Leaf Curl Disease ◽  
Leaf Curl

Evaluation of tomato inbred lines for resistance to the tomato yellow leaf curl disease complex in Oman

2018 ◽  
Vol 110 ◽  
pp. 91-98 ◽  
Author(s):  
Adel A. Al-Shihi ◽  
Peter Hanson ◽  
Abdullah M. Al-Sadi ◽  
Rashid A. Al-Yahyai ◽  
Rob W. Briddon ◽  
...  
Keyword(s):  
Inbred Lines ◽  
Tomato Yellow Leaf ◽  
Yellow Leaf ◽  
Disease Complex ◽  
Leaf Curl Disease ◽  
Leaf Curl

scholarly journals Roles and interactions of begomoviruses and satellite DNAs associated with okra leaf curl disease in Mali, West Africa

2009 ◽  
Vol 90 (4) ◽  
pp. 1001-1013 ◽  
Author(s):  
Tatsuya Kon ◽  
Maria R. Rojas ◽  
Issoufou K. Abdourhamane ◽  
Robert L. Gilbertson
Keyword(s):  
West Africa ◽  
Symptom Severity ◽  
Monopartite Begomoviruses ◽  
West African ◽  
Cotton Leaf ◽  
Satellite Dnas ◽  
Rep Protein ◽  
Major Constraint ◽  
Leaf Curl Disease ◽  
Leaf Curl

Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production in West Africa. Two monopartite begomoviruses (okra virus-1 and okra virus-2), a betasatellite and a DNA1 satellite are associated with OLCD in Mali. Okra virus-1 is an isolate of okra yellow crinkle virus (OYCrV), okra virus-2 is a recombinant isolate of cotton leaf curl Gezira virus (CLCuGV) and the betasatellite is a variant of cotton leaf curl Gezira betasatellite (CLCuGB). Cloned DNA of OYCrV and CLCuGV were infectious and induced leaf curl symptoms in Nicotiana benthamiana plants, but did not induce OLCD in okra. However, when these clones were individually co-inoculated with the cloned CLCuGB DNA, symptom severity and viral DNA levels were increased in N. benthamiana plants and typical OLCD symptoms were induced in okra. The CLCuGB was also replicated by, and increased symptom severity of, three monopartite tomato-infecting begomoviruses, including two from West Africa. The sequence of the DNA1 satellite was highly divergent, indicating that it represents a distinct West African lineage. DNA1 replicated autonomously, and replication required the DNA1-encoded Rep protein. Although DNA1 reduced helper begomovirus DNA levels, symptoms were not attenuated. In the presence of CLCuGB, DNA levels of the helper begomoviruses and DNA1 were substantially increased. Together, these findings establish that OLCD in Mali is caused by a complex of monopartite begomoviruses and a promiscuous betasatellite with an associated parasitic DNA1 satellite. These findings are discussed in terms of the aetiology of OLCD and the evolution of new begomovirus/satellite DNA complexes.

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