EMBRYONIC EXPOSURE TO O,P′-DDT CAUSES EGGSHELL THINNING AND ALTERED SHELL GLAND CARBONIC ANHYDRASE EXPRESSION IN THE DOMESTIC HEN

2006 ◽  
Vol 25 (10) ◽  
pp. 2787 ◽  
Author(s):  
Lena Holm ◽  
Alexandra Blomqvist ◽  
Ingvar Brandt ◽  
Björn Brunström ◽  
Yvonne Ridderstråle ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Shell Gland ◽  
Embryonic Exposure ◽  
Eggshell Thinning ◽  
Domestic Hen

scholarly journals Embryonic exposure to oestrogen causes eggshell thinning and altered shell gland carbonic anhydrase expression in the domestic hen

Reproduction ◽  
2004 ◽  
Vol 128 (4) ◽  
pp. 455-461 ◽  
Author(s):  
C Berg ◽  
A Blomqvist ◽  
L Holm ◽  
I Brandt ◽  
B Brunström ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Environmental Concern ◽  
Carbonic Anhydrase Activity ◽  
Shell Gland ◽  
In Ovo ◽  
Functional Disturbance ◽  
Embryonic Exposure ◽  
Eggshell Thinning ◽  
Resistance To Deformation ◽  
Domestic Hen

Eggshell thinning among wild birds has been an environmental concern for almost half a century. Although the mechanisms for contaminant-induced eggshell thinning are not fully understood, it is generally conceived to originate from exposure of the laying adult female. Here we show that eggshell thinning in the domestic hen is induced by embryonic exposure to the synthetic oestrogen ethynyloestradiol. Previously we reported that exposure of quail embryos to ethynyloestradiol caused histological changes and disrupted localization of carbonic anhydrase in the shell gland in the adult birds, implying a functional disturbance in the shell gland. The objective of this study was to examine whether in ovo exposure to ethynyloestradiol can affect eggshell formation and quality in the domestic hen. When examined at 32 weeks of age, hens exposed to ethynyloestradiol in ovo (20 ng/g egg) produced eggs with thinner eggshells and reduced strength (measured as resistance to deformation) compared with the controls. These changes remained 14 weeks later, confirming a persistent lesion. Ethynyloestradiol also caused a decrease in the number of shell gland capillaries and in the frequency of shell gland capillaries with carbonic anhydrase activity. These data suggested that a disrupted carbonic anhydrase expression was involved in the mechanism for the oestrogen-induced eggshell thinning found in this study. The results support our hypothesis that eggshell thinning in avian wildlife can result from a structural and functional malformation in the shell gland, induced by xeno-oestrogen exposure during embryonic development.

scholarly journals Shell Strength and Carbonic Anhydrase Activity of the Shell Gland of the Domestic Fowl

1968 ◽  
Vol 47 (3) ◽  
pp. 858-862 ◽  
Author(s):  
P.J. Heald ◽  
Dianne Pohlman ◽  
E.G. Martin
Keyword(s):  
Carbonic Anhydrase ◽  
Domestic Fowl ◽  
Carbonic Anhydrase Activity ◽  
Shell Gland

scholarly journals LOCALIZATION OF CARBONIC ANHYDRASE IN AVIAN GASTRIC MUCOSA, SHELL GLAND AND BONE BY IMMUNOHISTOCHEMISTRY

1974 ◽  
Vol 22 (8) ◽  
pp. 819-825 ◽  
Author(s):  
CAROL V. GAY ◽  
HARALD SCHRAER ◽  
EDWARD J. FALESKI ◽  
ROSEMARY SCHRAER
Keyword(s):  
Carbonic Anhydrase ◽  
Fluorescent Antibody ◽  
Fluorescein Isothiocyanate ◽  
Shell Gland ◽  
Significant Finding ◽  
Specific Fluorescence ◽  
Tubular Gland ◽  
Γ Globulins ◽  
Mucosal Lining ◽  
Avian Erythrocyte

Antisera to purified avian erythrocyte carbonic anhydrase (CA) were produced in rabbits and the γ-globulin fractions were isolated and purified by ammonium sulfate precipitation and diethylaminoethyl-Sephadex chromatography. The anti-CA immuno-γ-globulins were tested by immunoelectrophoresis and judged to be highly specific for CA. Fluorescein isothiocyanate-conjugated goat antirabbit γ-globulins were used in the indirect fluorescent antibody localization of CA in cryostat sections. Specific fluorescence of CA was observed in gastric mucosal lining cells, shell gland columnar and tubular gland cells, red blood cells, erythroblasts and osteoclasts. Specific fluorescence was absent in the several immunologic controls, including the blocking test. Specific fluorescence was also lacking in tissue constituents which contain no detectable amounts of CA, i.e., muscle, connective tissue, blood vessels and nuclei. A significant finding in this study is the occurrence of cross-reactions between antibodies to red blood cell CA and CA from other tissues, indicating the immunologic similarity of CA from different tissue sources.

Disrupted carbonic anhydrase distribution in the avian shell gland following in ovo exposure to estrogen

2001 ◽  
Vol 75 (6) ◽  
pp. 362-368 ◽  
Author(s):  
Lena Holm ◽  
Cecilia Berg ◽  
Björn Brunström ◽  
Yvonne Ridderstråle ◽  
Ingvar Brandt
Keyword(s):  
Carbonic Anhydrase ◽  
Shell Gland ◽  
In Ovo

A CYTOLOGICAL AND CYTOCHEMICAL STUDY OF THE SHELL GLAND OF THE DOMESTIC HEN

1953 ◽  
Vol 31 (6) ◽  
pp. 577-589 ◽  
Author(s):  
D. J. McCallion
Keyword(s):  
Shell Gland ◽  
Ciliated Epithelium ◽  
Shell Formation ◽  
Cytochemical Study ◽  
Negative Results ◽  
Inner Surface ◽  
Secretory Products ◽  
Egg Shell ◽  
Domestic Hen ◽  
Positive Results

A cytological and cytochemical study has been made of the oviducts of hens producing thick shelled eggs and of hens producing thin shelled eggs, with special reference to the shell gland. No significant differences between them were discovered. The inner surface of the oviduct of the hen is lined with ciliated epithelium containing two types of cells, ciliated and goblet. Lying under the ciliated lining are the tubular glands of the oviduct. The Golgi apparatus has been described in the several types of cells. The secretory products of these cells have been identified in the various parts of the oviduct. Small amounts of calcium were shown to be present in the oviduct by one of several techniques, the others giving negative results. Slight positive results were obtained for the presence of phosphates. Alkaline phosphatase was present in slight and varying amounts uniformly distributed in the oviduct, but acid phosphatase was confined to the ciliated epithelium. Glycogen was not present in the glandular tissues. The deposition of calcium as egg shell seems to be different from that in bone formation. Possible mechanisms of egg shell formation are discussed.

Localisation of Carbonic Anhydrase in the Sperm Storing Regions of the Domestic Hen Oviduct

1996 ◽  
Vol 156 (4) ◽  
pp. 253-260 ◽  
Author(s):  
L. Holm ◽  
Y. Ridderstrale ◽  
P.-G. Knutsson
Keyword(s):  
Carbonic Anhydrase ◽  
Domestic Hen
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