Embryonic valproate exposure alters mesencephalic dopaminergic neurons distribution and septal dopaminergic gene expression in domestic chicks

In recent years, the role of the dopaminergic system in the regulation of social behavior is being progressively outlined, and dysfunctions of the dopaminergic system are increasingly associated with neurodevelopmental disorders, including ASD. To further elucidate the role of the dopaminergic system in ASD, we investigated the effects of embryonic exposure to valproic acid (VPA) on the postnatal development of the mesencephalic DA system in the domestic chick. We found that VPA affected the rostro-caudal distribution of DA neurons, without changing the expression levels of several dopaminergic markers in the mesencephalon. We also investigated a potential consequence of this altered DA neuronal distribution in the septum, a social brain area previously associated to social behaviour in several vertebrate species, describing alterations in the expression of genes linked to DA neurotransmission. These findings support the emerging hypothesis of a role of DA dysfunction in ASD pathogenesis. Together with previous studies showing impairments of early social orienting behaviour, these data also support the use of the domestic chick model to investigate the neurobiological mechanisms involved in early ASD symptoms.

Dormant plasticity of rotifer diapausing eggs in response to predator kairomones

In freshwater ecosystems, hatching strategy of diapausing eggs (DEs) under predation risk has important ecological implication for zooplankters. Although kairomones released by predators can induce phenotypic responses of prey, hatching patterns of DEs in response to kairomones have received contradictory conclusions in zooplankters. Maternal environment may also affect hatching strategy of DEs during predator–prey interactions. We used classical Brachionus calyciflorus – Asplanchna models to determine the timing and proportion of DE hatching in association with parental and embryonic exposure to kairomones. Results obtained from two Brachionus clones supported the hypothesis that DEs could detect Asplanchna kairomones and adjust hatching patterns. DEs showed early and synchronous hatching patterns in the environment with kairomones. Data also supported the prediction that DEs could gain information about predators from maternal environments and adjusted their hatching pattern in response to the presence of kairomones. Compared with DEs from Brachionus mothers not exposed to kairomones, DEs produced by mothers that were experienced with kairomones attained a higher hatching rate when both of them hatched in the environment either with or without kairomones. Our results suggest that DEs of B . calyciflorus possess dormant plasticity to defend against predation from Asplanchna , which may be regulated by maternal environmental effects during sexual life cycles.

Early Embryo Exposure to Assisted Reproductive Manipulation Induced Subtle Changes in Liver Epigenetics with No Apparent Negative Health Consequences in Rabbit

Embryo manipulation is a requisite step in assisted reproductive technology (ART). Therefore, it is of great necessity to appraise the safety of ART and investigate the long-term effect, including lipid metabolism, on ART-conceived offspring. Augmenting our ART rabbit model to investigate lipid metabolic outcomes in offspring longitudinally, we detected variations in hepatic DNA methylation ART offspring in the F3 generation for embryonic exposure (multiple ovulation, vitrification and embryo transfer). Through adult liver metabolomics and proteomics, we identified changes mainly related to lipid metabolism (e.g., polyunsaturated fatty acids, steroids, steroid hormone). We also found that DNA methylation analysis was linked to changes in lipid metabolism and apoptosis genes. Nevertheless, these differences did not apparently alter the general health status. Thus, our findings suggest that ART is likely to be a player in embryo epigenetic events related to hepatic homeostasis alteration in adulthood.