Ridge Preservation With or Without an Osteoinductive Allograft: A Clinical, Radiographic, Micro-Computed Tomography, and Histologic Study Evaluating Dimensional Changes and New Bone Formation of the Alveolar Ridge

Objective: This study presents the histomorphometric findings after tooth extraction with and without Alveolar Ridge Preservation (ARP) with a collagen cone filling the socket in combination with a collagen membrane covering the socket. Materials and Methods: In a controlled randomized clinical study, 10 patients were treated with the combination material after tooth extraction. In 10 patients, the extraction sockets were left to heal without further intervention. Soft tissue, new bone formation, bone quality and bone remodeling, blood flow vascularization, and inflammation were evaluated histomorphometrically. This was performed (semi-) quantitatively using a blinded protocol. Results: The statistical evaluation showed no significant difference for any parameter. When the combination material was used, more pronounced remodeling, increased osteoblast activity, and increased vascularization were demonstrated based on the histomorphometric findings. In contrast, there were reduced levels of osteogenesis and less mineralization. There was slightly more bundle bone in patients with ARP. Conclusion: The histomorphometric analysis of ARP with a combination material consisting of a collagen cone and a collagen membrane showed no significant differences in terms of new bone formation and bone quality. Descriptively, however, different manifestations were seen that might benefit from being documented using larger samples and being tested for clinical relevance.

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Alveolar ridge dimensional changes following ridge preservation procedure using SocketKAP™: exploratory study of serial cone-beam computed tomography and histologic analysis in canine model

Clinical Oral Implants Research ◽

10.1111/clr.12711 ◽

2015 ◽

Vol 27 (9) ◽

pp. 1144-1151 ◽

Cited By ~ 4

Author(s):

Kyung-Ho Ryu ◽

Seiko Min ◽

Hyung-Keun You ◽

Yeon-Woo Sin ◽

Wan Lee ◽

...

Keyword(s):

Computed Tomography ◽

Cone Beam Computed Tomography ◽

Exploratory Study ◽

Canine Model ◽

Cone Beam ◽

Ridge Preservation ◽

Alveolar Ridge ◽

Dimensional Changes ◽

Histologic Analysis

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Topical co‐administration of zoledronate with recombinant human bone morphogenetic protein-2 can induce and maintain bone formation in the bone marrow environment

BMC Musculoskeletal Disorders ◽

10.1186/s12891-021-03971-w ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Hideki Ueyama ◽

Yoichi Ohta ◽

Yuuki Imai ◽

Akinobu Suzuki ◽

Ryo Sugama ◽

...

Keyword(s):

Bone Marrow ◽

Bone Formation ◽

Bone Structure ◽

Precursor Cells ◽

The Other ◽

Bone Morphogenetic Protein 2 ◽

New Bone Formation ◽

Micro Computed Tomography ◽

Mineral Density ◽

Left Femur

Abstract Background Bone morphogenetic proteins (BMPs) induce osteogenesis in various environments. However, when BMPs are used alone in the bone marrow environment, the maintenance of new bone formation is difficult owing to vigorous bone resorption. This is because BMPs stimulate the differentiation of not only osteoblast precursor cells but also osteoclast precursor cells. The present study aimed to induce and maintain new bone formation using the topical co-administration of recombinant human BMP-2 (rh-BMP-2) and zoledronate (ZOL) on beta-tricalcium phosphate (β-TCP) composite. Methods β-TCP columns were impregnated with both rh-BMP-2 (30 µg) and ZOL (5 µg), rh-BMP-2 alone, or ZOL alone, and implanted into the left femur canal of New Zealand white rabbits (n = 56). The implanted β-TCP columns were harvested and evaluated at 3 and 6 weeks after implantation. These harvested β-TCP columns were evaluated radiologically using plane radiograph, and histologically using haematoxylin/eosin (H&E) and Masson’s trichrome (MT) staining. In addition, micro-computed tomography (CT) was performed for qualitative analysis of bone formation in each group (n = 7). Results Tissue sections stained with H&E and MT dyes revealed that new bone formation inside the β-TCP composite was significantly greater in those impregnated with both rh-BMP-2 and ZOL than in those from the other experimental groups at 3 and 6 weeks after implantations (p < 0.05). Micro-CT data also demonstrated that the bone volume and the bone mineral density inside the β-TCP columns were significantly greater in those impregnated with both rh-BMP-2 and ZOL than in those from the other experimental groups at 3 and 6 weeks after implantations (p < 0.05). Conclusions The topical co-administration of both rh-BMP-2 and ZOL on β-TCP composite promoted and maintained newly formed bone structure in the bone marrow environment.

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Alveolar ridge dimensional changes following ridge preservation procedure with novel devices: Part 1 – CBCT linear analysis in non‐human primate model

Clinical Oral Implants Research ◽

10.1111/clr.12521 ◽

2015 ◽

Vol 27 (1) ◽

pp. 97-105 ◽

Cited By ~ 13

Author(s):

Seiko Min ◽

Yi Liu ◽

Jianxia Tang ◽

Yilin Xie ◽

Jimin Xiong ◽

...

Keyword(s):

Linear Analysis ◽

Ridge Preservation ◽

Alveolar Ridge ◽

Primate Model ◽

Dimensional Changes ◽

Novel Devices ◽

Human Primate

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Application of Bovine Acellular Cancellous Bone Matrix in Alveolar Ridge Preservation Following Tooth Extraction

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2021.2602 ◽

2021 ◽

Vol 11 (5) ◽

pp. 805-812

Author(s):

Hongguang Zhu ◽

Jianwen Bai ◽

Meirong Wei ◽

Ti Li

Keyword(s):

Bone Formation ◽

Cancellous Bone ◽

Tooth Extraction ◽

Bone Matrix ◽

Three Dimensional ◽

Ridge Preservation ◽

Alveolar Ridge ◽

Bone Powder ◽

Bone Substitute Materials ◽

Main Component

Objective: In this article, we explored the microscopic structure and composition of the decellular-ized cancellous bone matrix of the calf, and established the animal model of Beagle dog extraction. By applying different bone substitute materials in the extraction of teeth, we observed the new collagen-rich in the preservation of the site after tooth extraction. The protein bone matrix maintained the three-dimensional shape of the alveolar ridge compared to other biological materials. Methods: The microstructure of the new collagen-rich bone matrix was observed by scanning electron microscopy. The porous structure, porosity and distribution of collagen fibers were observed. XRD and infrared spectroscopy were used to further detect the inorganic and organic components in the new collagen-rich bone matrix. The premolar extraction and site preservation model of Beagle dogs were constructed. The changes of collagen-rich bone matrix, Bio-oss bone powder, CGF filling and blank control alveolar ridge volume were compared by CBCT. HE staining was used to observe and compare new bone formation, bone remodeling and bone resorption between groups, and to observe the formation of blood vessels, osteogenic mineralization, trabecular bone formation and inflammatory response in different periods. Results: (1) The acellular bone matrix of bovine cancellous bone completely removes the immunogenicity of the cells and has good histocompat-ibility; the pore diameter and porosity closest to the physiological structure, the main component is hydroxyapatite and collagen. (2) Site preservation can reduce the absorption of alveolar ridge following tooth extraction, preserve sufficient bone mass for alveolar fossa, and retain a good width of attachment, which provides further protection for implant surgery. (3) In the post-extraction site preservation, the CGF group has better bone composition than the BABM group and Bio-oss bone powder. Conclusion: Bovine Acellular Cancellous Bone Matrix is a new type of biological bone matrix. The main components are collagen and hydroxyapatite, which can promote bone formation in the extraction socket.

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The Effect of Graft Pretensioning on Bone Tunnel Diameter and Bone Formation After Anterior Cruciate Ligament Reconstruction in a Rat Model: Evaluation With Micro–Computed Tomography

The American Journal of Sports Medicine ◽

10.1177/0363546516686967 ◽

2017 ◽

Vol 45 (6) ◽

pp. 1349-1358 ◽

Cited By ~ 4

Author(s):

Jian-Chun Zong ◽

Richard Ma ◽

Hongsheng Wang ◽

Guang-Ting Cong ◽

Amir Lebaschi ◽

...

Keyword(s):

Bone Formation ◽

Acl Reconstruction ◽

Cruciate Ligament ◽

Bone Tunnel ◽

New Bone Formation ◽

Micro Computed Tomography ◽

Load To Failure ◽

Tunnel Diameter ◽

Anterior Cruciate ◽

Bone Tunnels

Background: Moderate graft pretensioning in anterior cruciate ligament (ACL) reconstruction is paramount to restore knee stability and normalize knee kinematics. However, little is known about the effect of graft pretensioning on graft-to-bone healing after ACL reconstruction. Hypothesis: Moderate graft pretensioning will improve bone formation within the bone tunnel after ACL reconstruction, resulting in superior load to failure. Study Design: Controlled laboratory study. Methods: 67 male Sprague-Dawley rats underwent unilateral ACL reconstruction with a flexor digitorum longus tendon autograft. The graft was subjected to pretensioning forces of 0 N, 5 N, or 10 N. Custom-made external fixators were used for knee immobilization postoperatively. Rats were euthanized for biomechanical load-to-failure testing (n = 45) and micro–computed tomography (μCT) examination (n = 22) at 3 and 6 weeks after surgery. Three regions of each femoral and tibial bone tunnel (aperture, middle, and tunnel exit) were chosen for measurement of tunnel diameter and new bone formation. Results: Biomechanical tests revealed significantly higher load-to-failure in the 5-N graft pretensioned group compared with the 0- and 10-N groups at 3 weeks (8.58 ± 2.67 N vs 3.96 ± 1.83 N and 4.46 ± 2.62 N, respectively) and 6 weeks (16.56 ± 3.50 N vs 10.82 ± 1.97 N and 7.35 ± 2.85 N, respectively) after surgery ( P < .05). The mean bone tunnel diameters at each of the 3 regions were significantly smaller in the 5-N group, at both the femoral and tibial tunnel sites, than in the 0- and 10-N groups ( P < .05). At 3 and 6 weeks postoperatively, the bone mineral density, bone volume fraction, and connectivity density around the aperture and middle regions of the tibial bone tunnels were all significantly higher in the 5-N group compared with the 0- and 10-N groups ( P < .05). In the aperture and middle regions of the femoral bone tunnels, pretensioning at either 5 or 10 N resulted in increased bone formation compared with the nonpretensioned group at 3 weeks postoperatively. No differences were found in bone formation between any of the 3 femoral tunnel regions at 6 weeks. Conclusion: Graft pretensioning can stimulate new bone formation and improve tendon-to-bone tunnel healing after ACL reconstruction. Clinical Relevance: Optimal graft pretensioning force in ACL reconstruction can improve bone tunnel healing. Further study is necessary to understand the mechanisms underlying the effect of graft pretensioning on healing at the bone-tunnel interface.

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