scholarly journals The Efficacy of Erbium Laser in Infected Root Canals: ex vivo study

2017 ◽  
Vol 13 (30) ◽  
pp. 453
Author(s):  
Agime Dragidella ◽  
Mira Jankullovska ◽  
Xhevdet Aliu ◽  
David Stubljar

Background: Golden standard of cleaning infected root canals with irrigants does not completely remove pathogens. The aim of our study was to estimate efficacy of Er:YAG laser compared to standard irrigation with NaOCl in infected root canals. Methods: 140 extracted premolar singlerooted teeth were used. The crowns were cut off so that we obtained 15 mm of root canal. Samples were divided into four groups and inoculated with Enterococcus faecalis, Candida albicans, Streptococcus sanguinis, Fusobacterium nucleatum. As disinfecting methods we performed Er:YAG laser radiation and irrigation with 5.20% NaOCl. Viability of microorganisms was analyzed using fluorescence by flow cytometry. Results: Both methods effectively eliminated major percent of microorganisms. Statistical differences were observed between tested organisms (p<0.05) for Er:YAG laser (30 and 90 seconds) and 5.20% NaOCl irrigation. The number of dead microorganisms was significantly higher for C. albicans and S. sanguinis than for E. faecalis or F. nucleatum. Longer duration of laser radiation of 90 seconds showed significant efficiency compared to 30-seconds radiation (p<0.001) and achieved over 80% of dead microbial cells. Disinfecting activity was even better in combination with irrigants and achieved over 90% in addition of NaOCl. Conclusions: Irradiation with Er:YAG laser could be used as a simple and standard disinfection method in endodontics, or even better can be used as as adjuvant therapy to standard and irrigation treatment.

2015 ◽  
Vol 17 (2) ◽  
pp. 43
Author(s):  
Rodolfo Zeledón DDS, Esp ◽  
Carolina Ballestero DDS, Esp ◽  
Erick Hernández DDS. Esp ◽  
Tatiana Ramírez DDS, MSD ◽  
Marianela Benavides DDS, Esp ◽  
...  

The objective of this clinical study was to evaluate by polymerase chain reaction (PCR) the antimicrobial efficacy and the outcome of an endodontic protocol treatment (EPT) performed by under-graduated dental students on infected root canals associated with periapical lesions. Fifty-six patients attending for treatment of pulp necrosis and apical periodontitis were included. A specific EPT approach was performed and DNA extracts were taken at the baseline (S1), after a disinfection-neutralization procedure (S2), post-chemomechanical preparation (S3) and after Intracanal medication (S4) for the presence of specific bacteria. The outcome of the EPT was assessed radiographically using the Periapical Index-system (PAI) after 18-month follow-up. An intergroup evaluation shows that when comparing S1 to S3, S1 to S4, S2 to S3, and S2 to S4, the presence of bacteria were significantly reduced (P < 0.05). Finally, S4 was not significantly reduced when compared with S3 (P > 0.05). The most prevalent species in their respect order were Actinomyces Israelii > Enterococcus Faecalis > Fusobacterium Nucleatum/Prevotella Nigrescens > Phorphyromonas Endodontalis. After the 18-month follow-up, the overall success rate of root canal – treated teeth was of 88% (PAI 1 or 2). This In Vivo study demonstrated that EPT significantly reduced the number of cases with positive results for the studied bacteria, showing a profound positive impact in the outcome of endodontic treatment of teeth diagnosed with Pulp Necrosis and Apical Periodontitis. 


2016 ◽  
Vol 50 (9) ◽  
pp. 910-918 ◽  
Author(s):  
İ. Kaşıkçı Bilgi ◽  
İ. Köseler ◽  
P. Güneri ◽  
M. Hülsmann ◽  
M. K. Çalışkan

2014 ◽  
Vol 508 ◽  
pp. 012026
Author(s):  
E Pedullà ◽  
C Genovese ◽  
C Scolaro ◽  
M Cutroneo ◽  
G Tempera ◽  
...  
Keyword(s):  
Ex Vivo ◽  

Author(s):  
Luciana Viti Betti ◽  
Clovis Monteiro Bramante ◽  
Ivaldo Gomes de Moraes ◽  
Norberti Bernardineli ◽  
Roberto Brandão Garcia

2003 ◽  
Author(s):  
Marcelo Sampaio Moura ◽  
Fatima A. A. Zanin ◽  
Aldo Brugnera, Jr. ◽  
Cyntia Rodrigues de Araujo Estrela ◽  
Carlos Estrela ◽  
...  

BDJ Open ◽  
2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Maayan Shacham ◽  
Avi Levin ◽  
Avi Shemesh ◽  
Alex Lvovsky ◽  
Joe Ben Itzhak ◽  
...  

AbstractThe aim of the current study was to determine the accuracy of electronic apex locator (EAL) measurements when using files of different sizes in roots with wide apical foramina while considering a new parameter of stability of EAL reading. Ten teeth with straight roots were subjected to a sequential widening of the apical foramen to 0.6, 0.7, and 0.8 mm. The roots were embedded after each enlargement stage in an alginate mold and subjected to EAL readings. Measurements were done using sequential K-file sizes and the self-adjusting file (SAF). Measurement stability was introduced as a new additional parameter. As the difference between the file size used and the apical diameter of the canal decreases, the results obtained were more accurate and stable. The stability and accuracy of the measurements coincided with each other in a statistically significant manner. Within the limitations of the present ex vivo study, it may be concluded that in straight canals with wide apical foramina of 0.6–0.8 mm, both SS K-files which fit snugly to the walls of apical foramen and the SAF file may offer both accurate and stable EAL measurements.


Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Fauzia Ashraf ◽  
Pushpa Shankarappa ◽  
Abhinav Misra ◽  
Asheesh Sawhney ◽  
Nandamuri Sridevi ◽  
...  

Introduction. The aim of the present study was to evaluate the dentinal cracks after root canal preparation with rotary files: Gates Glidden, ProTaper Universal, ProTaper Next, and HyFlex CM at different instrumentation lengths.Methodology. Sixty-five mandibular premolars were mounted in the acrylic tube with simulated periodontal ligaments and the apex was exposed. The root canals were instrumented with different rotary files, namely, ProTaper Universal, ProTaper Next, and HyFlex CM, to the major apical foramen (AF), short AF, and beyond AF. The root apex was stained with 1% methylene blue dye and digital images of apical surface of every tooth were taken and development of dentinal defects was determined by using stereomicroscope. Multinomial logistic regression test was performed to identify influencing factors.Results. Instrumentation with rotary files terminated 2 mm short AF and did not cause any cracks. Significantly less cracks were seen when instrumentation with rotary files terminated 1 mm short apical foramen when compared with the instrumentation terminated at or beyond apical foramen (p<0.05).Conclusion. ProTaper Universal rotary files caused more dentinal cracks than ProTaper Next and HyFlex CM. Instrumentation short AF reduced the risk of dentinal defects.


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