scholarly journals Micropropagation of White Palash tree (Butea monosperma (Lam.) Taub. Var. lutea (Witt.)).

2017 ◽  
Vol 9 ◽  
Author(s):  
Rathnaprabha D ◽  
Muralikrishna N ◽  
Raghu E ◽  
Yashodhara V ◽  
Sadanandam A
Keyword(s):  
High Frequency ◽  
Multiple Shoots ◽  
Time Interval ◽  
Ms Medium ◽  
Direct Shoot Regeneration ◽  
Regeneration Medium ◽  
Butea Monosperma ◽  
Cotyledonary Nodes ◽  
Mature Seeds

<p class="Default"><span>An efficient and reproducible protocol is established for rapid <em>in vitro</em> multiplication of an endangered, valuable medicinal plant, <em>Butea monosperma </em>(Lam.) Taub. Var. <em>lutea</em>, through cotyledonary nodes of mature seeds. Among various cytokinins tested, high frequency of direct shoot regeneration was induced on Murashige and skoog (MS) medium supplemented with BAP, which found to be more effective and showed optimal response at 2 mg/L with a maximum number of </span><span>8.35±0.32 multiple shoots per explant. Proliferation of shoots was established by repeated subculturing on to same regeneration medium with 2-3 weeks of time interval. Rooting of regenerated shoots was achieved after 3 weeks of culture on MS medium containing 1 mg/L IBA. <em>In vitro</em> raised plantlets were transferred to pots containing sterilized soil and vermiculate mixture in 1:1 ratio and then shifted to greenhouse. Well established plantlets exhibited 75% survival rate.</span></p>

scholarly journals In vitro propagation of muskmelon (Cucumis melo L.) from nodal segments, shoot tips and cotyledonary nodes

2015 ◽  
Vol 41 ◽  
pp. 71-77
Author(s):  
S. Parvin ◽  
M. Kausar ◽  
M. Enamul Haque ◽  
M. Khalekuzzaman ◽  
B. Sikdar ◽  
...  
Keyword(s):  
Cucumis Melo ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Ms Medium ◽  
Cotyledonary Nodes ◽  
Cucumis Melo L

A rapid and efficient protocol is outlined for in vitro propagation of muskmelon(Cucumis melo L.) Shoot tips, nodal segments and cotyledonary nodes from invitro grown seedlings were used as explants. The explants were inoculated on MS medium fortified with different combinations and concentrations of growthregulators viz., BAP, NAA, GA3 and IBA for multiple shoot regeneration.Effective result was found on MS medium supplemented with 2.0 mg/l BAP, inwhich 90% and 70% cultures induced multiple shoots from nodal segments andshoot tip explants, respectively. Whereas, 70% cultures of cotyledonary nodeswere found to induced shoots on MS medium with 1.5 mg/l BAP + 0.1 mg/l GA3. In vitro regenerated shoots were subcultured on half strength MS mediumsupplemented with different concentrations of IBA and NAA for successful rootinduction and the effective result (up to 70%) was found in medium with 1 mg/lIBA. Well rooted in vitro grown plantlets were acclimatized in sandy soil, whereas 70% plantlets survived

scholarly journals Effects of N-Benzyl -9-(2-tetrahydropyranyl and Indole –3-Acetic Acid In Vitro Culture of Bauhinia purpurea L.

2019 ◽  
pp. 238-242
Author(s):  
Belai Meeta Suwal Singh
Keyword(s):  
Analytical Procedure ◽  
Multiple Shoots ◽  
Nodal Explants ◽  
Leguminous Plant ◽  
Ms Medium ◽  
Half Strength ◽  
Bauhinia Purpurea ◽  
Mature Seeds ◽  
Indole 3 Acetic Acid

<p>Bauhinia purpurea L. is a leguminous plant moderate sized tree with multipurpose value. It is distributed in sub-Himalayan tracts. It has been cultivated in the plain region up to the elevation of 1350 m. Mature seeds of Bauhinia purpurea L. were cultured on half strength Murashige and Skoog (1962) (MS) medium. Nodal explants obtained from germinated seedlings were cultured on MS medium containing 0.5 M BAP produced multiple shoots which were used for experimental purposes. Nodal explants obtained from cultured were subcultured on different concentrations of N-Benzyl -9-(2-tetrahydropyranyl) (BPA) and Indole-3acetic acid (IAA). The best proliferation of nodes and shoots were observed on the MS medium supplemented with 0.5 M BPA and 0.1 M IAA. After 8 weeks of culture, the propagated plants were acclimatized and transferred to the sand box containing 1:1 soil and sand. Well rooted plants were then established in the field. All the data collected were worked out statistically with SPSS, a system of analytical procedure.</p>

scholarly journals In vitro clonal multiplication of Aegle marmelos (L.) Corr. through cotylodonary node culture

2013 ◽  
Vol 48 (1) ◽  
pp. 13-18 ◽  
Author(s):  
S Akter ◽  
TA Banu ◽  
MA Habib ◽  
S Afrin ◽  
A Khatun ◽  
...  
Keyword(s):  
Multiple Shoots ◽  
Poor Response ◽  
Induction Medium ◽  
Root Induction ◽  
Ms Medium ◽  
Aegle Marmelos ◽  
Clonal Multiplication ◽  
Cotyledonary Nodes ◽  
Half Strength

Clonal multiplication of Aegle marmelos was achieved through in vitro culture. Cotyledonary nodes from one month old in-vitro grown seedlings of Aegle marmelos were cultured on MS medium supplemented with BAP, Kn, and IBA either alone or in combination. The highest regenerative response was observed on medium containing 2.5 mg/L BAP and 0.5 mg/L Kn from node and shoot tip within 8-10 days. When regenerated shoots were subcultured on MS medium supplemented with 0.2 mg/L BAP and 0.02 mg/L Kn, maximum number of multiple shoots were observed after third phase of subculture. Poor response was found using MS medium supplemented with Kn only. In vitro induced shoots were transferred into root induction medium consisting of half-strength MS supplemented with auxins, IAA, IBA or NAA. Rooting was best in medium supplemented with 1.0 mg/L IBA. Rooted plantlets were acclimatized and transferred to the soil with 96% survival rate. DOI: http://dx.doi.org/10.3329/bjsir.v48i1.15408 Bangladesh J. Sci. Ind. Res. 48(1), 13-18, 2013

scholarly journals In vitro Plant Regeneration from Axillary Buds of Asparagus racemosus Wild, a Medicinal Plant

1970 ◽  
Vol 45 (3) ◽  
pp. 255-260
Author(s):  
F Afroz ◽  
MAA Jahan ◽  
AKM Sayeed Hassan ◽  
R Khatun
Keyword(s):  
Medicinal Plant ◽  
High Frequency ◽  
Shoot Proliferation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Asparagus Racemosus ◽  
Ms Medium ◽  
Multiple Shoot Induction ◽  
Multiple Shoot Regeneration

An efficient method was developed for regeneration of Asparagus racemosus Wild, from axillary explants. MS media supplemented with different concentrations of cytokinin (BAP) alone or in combination of different concentrations of auxin (NAA or IBA) was tested for their efficiency in multiple shoot induction. High frequency of multiple shoot regeneration was achieved on MS medium supplemented with BAP (0.1mg/l) and NAA (0.05 mg/l). When shoots were well developed they were dissected and subcultured on the same medium to promote more multiple shoots. Eighteen to twenty shoots were obtained on this medium. The shoots were rooted best on half MS medium supplemented with 0.05 mg/l BAP and 1.0 mg/l IBA. Among the five levels of pH tested, 5.7 was the best for multiple shoot proliferation. The result presented here proved to be suitable for the rapid propagation system of A. racemosus. Key words: Asparagus racemosus; Medicinal plant; Rapid proliferation; Multiple shoots; Auxins and Cytokinins DOI: 10.3329/bjsir.v45i3.6534Bangladesh J. Sci. Ind. Res. 45(3), 255-260, 2010

scholarly journals In vitro Plant Regeneration from Mature Seed Explants of Withania somnifera (L.) Dunal, an Important, Rare and Endangered Medicinal Plant

2019 ◽  
Vol 11 (4) ◽  
pp. 387-391
Author(s):  
Sape Subba TATA ◽  
Geddam JYOTHIRMAYEE ◽  
Owk Aniel KUMAR
Keyword(s):  
Medicinal Plant ◽  
Withania Somnifera ◽  
Multiple Shoots ◽  
Mature Seed ◽  
Direct Organogenesis ◽  
Ms Medium ◽  
Regeneration System ◽  
Time Saving ◽  
Mature Seeds

Withania somnifera (L.) Dunal a member of the Solanaceae family, is a traditional medicinal plant commonly known in India as Ashwagandha. It is used for different diseases such as hiccup, cough, rheumatism, tuberculosis, and exhibits excellent antitumor and anti-bacterial activities as well. Direct organogenesis of plants using mature seeds provides faster response and is also a time saving approach, thus the present study was conducted to investigate the optimal concentrations and combinations of plant growth regulators with MS medium for the establishment of an efficient regeneration system in W. somnifera using mature seed as an explant. Therefore, an efficient in vitro protocol for high frequency regeneration has been developed using mature seeds as explant. In the present study, the multiple shoots along with embryogenic callus induction was best seen in MS medium supplemented with BAP (1.5 mg/L) and IAA (0.5 mg/L). Furthermore, MS medium fortified with GA3 (0.3 mg/L) and IBA (3.0 mg/L) alone was suited for shoot elongation and rhizogenesis respectively. The rooted plantlets were hardened and successfully established in the soil. The establishment of a highly reproducible regeneration system would greatly influence the efforts of improvement of the hereby studied medicinal plant species through useful gene transfer technology.

Intensification de la régénération du pois (Pisum sativum L.), par le thidiazuron, via la formation de structures caulinaires organogènes

1997 ◽  
Vol 75 (3) ◽  
pp. 492-500 ◽  
Author(s):  
Delphine Popiers ◽  
Frédéric Flandre ◽  
Brigitte S. Sangwan-Norreel
Keyword(s):  
Pisum Sativum ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Cotyledonary Nodes ◽  
Pisum Sativum L ◽  
Embryo Axes ◽  
Initial Medium ◽  
Second Wave ◽  
Mature Seeds

In vitro regeneration of pea (Pisum sativum L.), a regeneration recalcitrant legume, was optimised using thidiazuron. Buds were initiated from the meristems of the cotyledonary nodes of embryo axes, isolated from mature seeds, and subcultured on Murashige and Skoog medium supplemented with 13.3 μM 6-benzylaminopurine, 16.1 μM α-naphthaleneacetic acid, and 0.2 μM 2,3,5-triiodobenzoic acid. Proliferation of buds was preceded by the formation of white nodular-like protrusions. These structures were cut transversally in fine slices and subcultured on the same medium or in presence of thidiazuron that produces a second wave of secondary budding. The best results (90–110 buds per expiant) were obtained with 10 μM thidiazuron. The capacity of regeneration was genotype independent and reproducible. Buds elongated on the initial medium, then formed roots in presence of 5.37 μM α-naphthaleneacetic acid. and developed into viable plants. Key words: Pisum sativum L., regeneration, meristems, embryo axes, thidiazuron.

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

scholarly journals In vitro Flowering of Shoots Regenerated from Cultured Nodal Explants

2011 ◽  
Vol 39 (1) ◽  
pp. 84 ◽  
Author(s):  
Kantamaht KANCHANAPOOM ◽  
Suttinee JINGJIT ◽  
Kamnoon KANCHANAPOOM
Keyword(s):  
Multiple Shoots ◽  
Shoot Formation ◽  
In Vitro Flowering ◽  
Nodal Explants ◽  
Callus Growth ◽  
Ms Medium ◽  
Old Field ◽  
Gypsophila Paniculata ◽  
Initial Culture

A protocol for the regeneration of Gypsophila paniculata L. using nodal explants from 2-month-old field grown plants was established. The induction of multiple shoots was best obtained on Murashige and Skoog (MS) medium supplemented with 13.3 μM BA. Callus growth was observed on MS medium containing 44.3 μM BA. Calluses were transferred to MS medium supplemented with 2, 4-D (4.5, 13.5, 22.6 μM), NAA (5.3, 16.1, 26.8 μM) or BA (4.4, 13.3, 22.1 μM) for 2 months to induce shoot formation. After 6 weeks of initial culture, multiple shoots were regenerated from calluses cultured on MS medium supplemented with 13.3 μM BA. All regenerated shoots produced roots on 16.1 μM NAA containing MS medium within 4 weeks. Rooted plantlets were hardened and established in pots at 100% survival. For induction of in vitro flowering, regenerated shoots could be induced to flower efficiently when cultured on MS medium containing 13.3 μM BA and 50 g/l sucrose.

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals In vitro bulblet regeneration from immature embryos of endangered and endemic Muscari azureum

2011 ◽  
Vol 63 (1) ◽  
pp. 209-215 ◽  
Author(s):  
S. Uranbey
Keyword(s):  
Callus Induction ◽  
High Frequency ◽  
Ornamental Plant ◽  
Immature Embryos ◽  
Induction Medium ◽  
Ms Medium ◽  
Culture Initiation ◽  
Mineral Salts ◽  
Font Color

A high frequency of bulblet regeneration was achieved for the endemic and endangered ornamental plant Muscari azureum using immature embryos. Immature embryos of M. azureum were cultured on a callus induction medium consisting of N6 mineral salts and vitamins, 400 gL-1 casein + 40 gL-1 sucrose + 2 mgL-1 L-proline, 2 mgL-1 2,4-D and 2 gL-1 Gelrite. Then the embryogenic callus clusters were transferred to a bulblet induction medium consisting of MS mineral salts and vitamins containing different concentrations and combinations of BAP, KIN, TDZ, Zeatin, IAA, NAA, 30 gL-1 sucrose and 7 gL-1 agar. Prolific bulblet multiplication (over 13 bulblets/embryo) was achieved from immature embryos after 5-6 months of culture initiation. Well-developed bulblets were excised and individually rooted on ? strength MS medium supplemented with 1 mgL-1 IBA, 0.5 gL-1activated charcoal, 20 gL-1sucrose and 6 gL-1agar and acclimatized. <br><br><font color="red"><b> This article has been retracted. Link to the retraction <u><a href="http://dx.doi.org/10.2298/ABS150608072E">10.2298/ABS150608072E</a><u></b></font>

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