scholarly journals In vitro bulblet regeneration from immature embryos of endangered and endemic Muscari azureum

2011 ◽  
Vol 63 (1) ◽  
pp. 209-215 ◽  
Author(s):  
S. Uranbey
Keyword(s):  
Callus Induction ◽  
High Frequency ◽  
Ornamental Plant ◽  
Immature Embryos ◽  
Induction Medium ◽  
Ms Medium ◽  
Culture Initiation ◽  
Mineral Salts ◽  
Font Color

A high frequency of bulblet regeneration was achieved for the endemic and endangered ornamental plant Muscari azureum using immature embryos. Immature embryos of M. azureum were cultured on a callus induction medium consisting of N6 mineral salts and vitamins, 400 gL-1 casein + 40 gL-1 sucrose + 2 mgL-1 L-proline, 2 mgL-1 2,4-D and 2 gL-1 Gelrite. Then the embryogenic callus clusters were transferred to a bulblet induction medium consisting of MS mineral salts and vitamins containing different concentrations and combinations of BAP, KIN, TDZ, Zeatin, IAA, NAA, 30 gL-1 sucrose and 7 gL-1 agar. Prolific bulblet multiplication (over 13 bulblets/embryo) was achieved from immature embryos after 5-6 months of culture initiation. Well-developed bulblets were excised and individually rooted on ? strength MS medium supplemented with 1 mgL-1 IBA, 0.5 gL-1activated charcoal, 20 gL-1sucrose and 6 gL-1agar and acclimatized. <br><br><font color="red"><b> This article has been retracted. Link to the retraction <u><a href="http://dx.doi.org/10.2298/ABS150608072E">10.2298/ABS150608072E</a><u></b></font>

scholarly journals PENGARUH JENIS EKSPLAN DAN KOMBINASI ZAT PENGATUR TUMBUH (ZPT) TERHADAP INDUKSI KALUS Begonia bimaensis Undaharta & Ardaka SECARA IN VITRO

2020 ◽  
Vol 23 (1) ◽  
pp. 82-90
Author(s):  
Ema Hendriyani ◽  
Tri Warseno ◽  
Ni Kadek Erosi Undaharta
Keyword(s):  
Plant Growth ◽  
Callus Induction ◽  
Plant Growth Regulator ◽  
Ornamental Plant ◽  
Induction Medium ◽  
Ms Medium ◽  
Leaf Lamina ◽  
Leaf Base ◽  
Callus Production

Begonia bimaensis Undaharta & Ardaka is a potential ornamental plant, and currently known only from one population in Sumbawa. Propagation programs, both conventional and in vitro culture are necessary to ensure its conservation. The aim of this research is to observe the effects of explant types and plant growth regulator combination (2,4-D and kinetin) in inducing callus from B. bimaensis leaf in vitro. Callus induction was initiated from three parts of leaf explant, namely petiole, leaf base, and leaf lamina. The explants were planted on Murashige & Skoog (MS) medium with addition of 2,4-D and kinetin. Concentrations of 2,4-D were 0, 0.5, and 1 ppm, while kinetin concentrations were 0, 1, and 2 ppm. Each treatment was replicated 10 times. Results showed that leaf base was the best explant used for callus induction. Medium D1K2 (MS + 1 ppm kinetin) showed the fastest time for callus induction that was at 20 days after planting. The highest percentage of callus production (100%) was found on D1K3 (MS + 2ppm kinetin); D2K2 (MS + 0.5ppm 2,4-D + 1 ppm kinetin); D2K3 (MS + 0.5ppm 2,4-D + 2ppm kinetin) and D3K2 (MS + 1ppm 2,4-D + 1ppm kinetin).

scholarly journals In vitro Regeneration of Dalbergia sissoo Roxb. and the Potential for Genetic Transformation

2012 ◽  
Vol 40 (2) ◽  
pp. 140 ◽  
Author(s):  
Hafiz Mamoon REHMAN ◽  
Iqrar Ahmad RANA ◽  
Siddra IJAZ ◽  
Ghulam MUSTAFA ◽  
Faiz Ahmad JOYIA ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Genetic Transformation ◽  
Callus Induction ◽  
In Vitro Regeneration ◽  
Induction Medium ◽  
Native Forest ◽  
Ms Medium ◽  
Dalbergia Sissoo ◽  
Callus Induction Medium

Dalbergia sissoo Roxb. ex DC. (Sissoo) is a native forest tree species in Pakistan. Many ecological and economical uses are associated with this premier timber species, but dieback disease is of major concern. The objective of this study was to develop a protocol for in vitro regeneration of Sissoo that could serve as target material for genetic transformation, in order to improve this species. Callus formation and plantlet regeneration was achieved by culturing cotyledons, immature seeds, and mature embryos on a modified Murashige and Skoog (1962) (MS) medium supplemented with plant growth regulators. Callus induction medium containing 2.71 ?M 2, 4-dichlorophenoxyacetic acid (2,4-D) and 0.93 ?M kinetin produced better callus on all explants tested compared to other treatments, such as 8.88 ?M 6-benzylaminopurine (BA) and 2.69 ?M ?-naphthalene acetic acid (NAA), or 2.71 ?M 2, 4-D and 2.69 ?M NAA. Shoot regeneration was best on MS medium containing 1.4 ?M NAA and 8.88 ?M BA compared to other treatments, such as 1.4 ?M NAA and 9.9 ?M kinetin, or 2.86 ?M indole-3-acetic acid and 8.88 ?M BA. Murashige and Skoog medium containing 1.4 NAA ?M and 8.88 ?M BA was better in general for regeneration regardless of callus induction medium and the type of explant used. Rooting was best on half-strength MS medium with 7.35 ?M indole-3-butyric acid. Regenerated plantlets were acclimatized for plantation in the field. Preliminary genetic transformation potential of D. sissoo was evaluated by particle bombardment of callus explants with a pUbiGus vector. The bombarded tissue showed transient Gus activity 1week after bombardment. Transformation of this woody tree is possible provided excellent regeneration protocols. The best combination for regeneration explained in this study is one of such protocols.

scholarly journals Mature Embryo-Based in vitro Regeneration of Indica Rice Cultivars for High Frequency Plantlets Production

2017 ◽  
Vol 20 (2) ◽  
pp. 81-87
Author(s):  
HN Barman ◽  
ME Hoque ◽  
RK Roy ◽  
PL Biswas ◽  
MAI Khan ◽  
...  
Keyword(s):  
Callus Induction ◽  
High Frequency ◽  
Indica Rice ◽  
In Vitro Regeneration ◽  
Mature Embryo ◽  
Ms Medium ◽  
Rice Varieties ◽  
Growing Medium ◽  
Regeneration Efficiency

The study was conducted at Biotechnology Division of Bangladesh Rice Research Institute (BRRI) to investigate the effects of plant growing medium and plant growth regulator (PGR) for the callus induction and high frequency plantlets regeneration of indica rice. Ten indica rice varieties viz. BR5, BR11, BRRI dhan28, BRRI dhan29, BRRI dhan33, BRRI dhan41, BRRI dhan47, BRRI dhan48, BRRI dhan49 and BRRI dhan50 were cultured on MS, N6 and LS media. The MS medium was found better for callus induction as compared to N6 and LS media. Among the tested varieties BRRI dhan48 induced the highest percent and best quality callus. Interaction effects of BRRI dhan48 to MS medium yielded 71.55% callus induction. The regeneration efficiency of BRRI dhan48 was tested on MS medium supplemented with different combination of NAA plus BAP and NAA plus kinetin. MS medium supplemented with 2.0 mg L-1 NAA and 2.0 mg L-1 Kn was found the best in respect of percent regenerated (76.67%) plantlet as well as for the growth of plantlets in vitro.Bangladesh Rice j. 2016, 20(2): 81-87

The Effects of Cytokinins on Plant Regeneration of Vetiver Grass (Vetiveria nemoralis A. Camus cv. Roiet)

2015 ◽  
Vol 804 ◽  
pp. 259-262
Author(s):  
Chonnikarn Khunchuay ◽  
Kanokporn Sompornpailin
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Induction Medium ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Regeneration Medium ◽  
Shoot Number ◽  
Induction Experiment

The optimum ratios of auxin and cytokinin are necessary for callus induction and plant regeneration. This ratio is a key function involving in the promoting cell division and proliferation in tissue culture. The axillary buds of in vitro plantlets fromVetiveria nemoralisA. Camuscv. Roiet were used as explants for the callus induction experiment. These explants were cultured on Murashige & Skoog (MS) medium [1] supplemented with various combinations of auxins and cytokinins. Under this experimental study, the highest frequency of callus induction was found on MS medium supplemented with 2 mgL-1α-naphthalene acetic acid (NAA) and 1 mgL-12-furanylmethyl-1H-purine-6-amine (kinetin) (62.5%). On the other hand the combination of 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 6-benzylaminopurine (BAP) was toxicity to this explants. All culturing explants were dead and no calli appearance. The calli derived from each medium were transferred into the same regeneration medium (MS with 1 mgL-1NAA and 2 mgL-1BAP). After culturing on regeneration medium, calli induced from the highest callus induction medium have shown high frequencies of regeneration and also shoot number per callus (58.33% and 7.1 shoots).

scholarly journals In vitro propagation of Codonopsis javania (Blume) Hook. f. et Thomson through the callus induction

2019 ◽  
Vol 2 (4) ◽  
pp. 56-61 ◽  
Author(s):  
Vi Thi Tuong Nguyen ◽  
Trinh Le Diem Ho ◽  
Kim Thi A Phan
Keyword(s):  
Callus Induction ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Induction Medium ◽  
Ms Medium ◽  
Root Yield ◽  
Yield Quality ◽  
Shoot Induction Medium

Codonopsis javania (Blume) Hook.f. et Thomson a traditional medicine plant and now an endangered species in Vietnam is grown for roots. The research was carried out to establish the plant propagation for the purpose of concerving and exploting this endangered medicinal herbs. In vitro shoot tip explants (1 – 1.5 cm) were induced to form callus on MS medium containing NAA (0.5 – 2 mg /L) with TDZ 0.1 mg/L. After four weeks of culture, in the MS medium combine with NAA 1 mg/L and TDZ 0.1 mg/L the explant induced compact callus (green, solid) wsa achieved 85.33%. The callus induction to form shoots on medium MS containing BA (0.5 – 2.0 mg/L) with NAA 0.2 mg/L. After 4 weeks of culture, shoot formation was higher in the MS medium containing BA 1.0 mg /L and NAA 0.2 mg/L and achieved of 82.67 % with 9.92 shoots/explant. The best shoot proliferation (2 – 3 cm) was excised and transferred to a medium shoot multiplication with the same composition as the shoot induction medium in which NAA 0.2 mg/L was replaced by NAA 0.5 mg/L. When compared the shoot multiplication between the two mediums at the same BA concentration (2 mg/L), all shoots increased and reached 5.87 times after 60 days cultured. On rooting MS medium with IBA 1 mg/L, 88.67 % in vitro rooting was observed with the average root yield of 4.33 roots/shoot and the length of 8.27 cm. Root length and their yield quality were highly improved when using of coconut fiber (30 %) and earthworms compost (70 %) (v/v) in the transfer medium after acclimatisation stages.

Establishment of a highly efficient regeneration system for in vitro culture of young wheat spikes

2006 ◽  
Vol 3 (2) ◽  
pp. 147-154
Author(s):  
Zhao Lin-Shu ◽  
Liu Lu-Xiang ◽  
Wang Jing ◽  
Zheng Qi-Cheng ◽  
Guo Hui-Jun ◽  
...  
Keyword(s):  
Callus Induction ◽  
Shoot Formation ◽  
Immature Embryos ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Regeneration System ◽  
Differentiation Medium ◽  
Efficient Regeneration ◽  
Young Spike

AbstractThis study used three winter wheat (Triticum aestivum L.) genotypes (H6756, H311 and SP8581) to compare the effects of sampling time, callus induction media, differentiation media and rooting media on in vitro culture of young spikes in wheat. In all these three genotypes, the frequencies of green plantlet differentiation were high when their young spikes were cultured between the stages of protective glume primordium formation and pistil and stamen primordium formation, but low at other stages. The optimum medium for callus induction was Murashige and Skoog (MS) medium+2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The optimum green plantlet differentiation medium was MS medium. Some abnormal plantlets regenerated from calli. When these plantlets were transferred to another differentiation medium [MS+1.0 mg/l 1-naphthaleneacetic acid (NAA)+0.2 mg/l 6-benzylaminopurine (6-BA)], shoot formation and elongation were induced. This allowed 90.91% of them to develop into normal green plantlets. The optimum rooting medium was 1/2MS+0.2 mg/l 3-Indolylacetonitrile (IAA)+80 g/l sucrose. An efficient regeneration system for young spike culture of wheat was set up based on such methods. Using this wheat-regeneration system, young spikes and immature embryos of 17 genotypes of wheat were in vitro cultured to study and compare the callus induction frequencies and green plantlet differentiation frequencies. The results of two successive years showed that in 15 out of the 17 genotypes (88.24%) the green plantlet differentiation frequencies were higher than those of immature embryos by 6.2–65.1%. These results showed that the regeneration system established in this trial for young spike culture of wheat was effective.

scholarly journals In vitro Micropropagation of Pomegranate (Punica granatum L.) Derived from Cotyledon

2021 ◽  
Vol 31 (1) ◽  
pp. 61-69
Author(s):  
MH Kabir ◽  
Pronabananda Das ◽  
ANK Mamun ◽  
Md Monirul Islam ◽  
Md Aminul Islam
Keyword(s):  
High Frequency ◽  
Punica Granatum ◽  
Ms Medium ◽  
In Vitro Plant Regeneration ◽  
Culture Initiation ◽  
In Vitro Micropropagation ◽  
Half Strength ◽  
Acclimatization Period ◽  
Punica Granatum L

A high frequency in vitro plant regeneration of pomegranate was established on MS medium supplemented with different concentrations and combinations of plant growth regulators. As explant cotyledons were employed for this study. Ninety percent of the cultured explants responded to form shoots from 30 days old in vitro raised seedlings after 90 days of culture initiation in MS containing 1.0 mg/l IBA + 0.1 mg/l NAA. The average number of shoots per explant was 10.0 ± 2.20, shoot length of 12.0 ± 2.40 cm, node per regenerated shoot was 9.0 ± 1.60 and the leaf number was14.0 ± 1.40. Well developed shoots were cultured on half strength of MS medium supplemented with 0.5 mg/l IBA, in which 90% shoot induced roots implanted after one month. The average number of root per shoot was 8.0 ± 0.90 and the average root length of 6.5 ± 0.40 cm was observed in this medium. Eighty percent plantlets were survived in the outdoor condition during the acclimatization period of seven days. Plant Tissue Cult. & Biotech. 31(1): 61-69, 2021 (June)

scholarly journals Callus induction in papaya (Carica papaya L.) and synseed production for low temperature storage and cryopreservation

2014 ◽  
Vol 26 (2) ◽  
pp. 155-162 ◽  
Author(s):  
Jaime A. Teixeira da Silva
Keyword(s):  
Low Temperature ◽  
Callus Induction ◽  
Carica Papaya ◽  
Alginate Beads ◽  
Induction Medium ◽  
Zygotic Embryos ◽  
Ms Medium ◽  
Low Temperature Storage ◽  
Temperature Storage

ABSTRACT The mid- to long-term preservation of papaya (Carica papaya L.) would allow for the safeguarding of important germplasm. In this study, soft friable callus (SFC) and hard callus (HC) were induced from the first two true leaves of 10-day-old seedlings containing a midrib derived from the germinated seed of two cultivars (‘Rainbow’ and ‘Sunrise Solo’). Following germination on a Murashige and Skoog (MS) medium that contained 3% sucrose and was free of plant growth regulators (PGRs), sections of the first true leaves from 10-day-old seedlings were exposed to seven published callus or somatic embryogenesis protocols for zygotic embryos, leaves or hypocotyls. Optimal SFC and HC induction was carried out on a half-strength MS medium following the Fitch (1993) or the Ascêncio-Cabral et al. (2008) protocol, respectively. SFC formed shoots that could then convert to plants when transferred to a full-strength MS medium devoid of PGRs. Plantlets 10-cm tall were acclimatised in two steps: first by in vitro acclimatisation in aerated vessels, the Vitron, under CO2-enriched (3000 ppm CO2), then by the transfer of individually rooted plantlets in Rockwool® blocks to a substrate of soil: pine bark : perlite (1:1:1, v/v/v). SFC and HC were then encapsulated in alginate beads, which were exposed to low temperature storage (LTS) at 10°C and 15°C, and also cryopreserved for 30 days. All encapsulated alginate beads that contained SFC, HC or leaf tissue that had been stored under LTS or cryopreserved were able to regenerate callus when placed on an optimal callus induction medium. Plants derived from the control, LTS and cryopreservation protocols, either from SFC or HC, were successfully acclimatised.

scholarly journals Callus induction and plant regeneration by Brazilian triticale and wheat genotypes

1997 ◽  
Vol 20 (1) ◽  
pp. 41-44 ◽  
Author(s):  
A.L.C. Dornelles ◽  
F.I.F. Carvalho ◽  
L.C. Federizzi ◽  
C.L. Handel ◽  
F. Bered ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Culture Media ◽  
Rio Grande ◽  
Immature Embryos ◽  
Ms Medium ◽  
Rio Grande Do Sul ◽  
Wheat Genotypes ◽  
The One

In order to determine the in vitro behavior of Brazilian triticale, 16 triticale genotypes, and three wheat genotypes used as checks, were sown in June 1994. The explants used were immature embryos. In addition to the genotype tests, two culture media for callus induction were also evaluated, i.e., MS (Murashige and Skoog, Physiol. Plant. 15: 473-497, 1962) medium containing 2.0 mg 2,4D/l, and MS medium containing 4.0 mg 2,4D/l. The plant regeneration protocol used was the one employed at the Laboratório de Cultura de Tecidos, Departamento de Plantas de Lavoura, Universidade Federal do Rio Grande do Sul, for wheat. Differences in plant regeneration were observed both among triticale and wheat genotypes, with triticale usually showing better regeneration than wheat. No differences were observed between the callus induction media.

scholarly journals In Vitro Propagation of Alexandrian Laurel (Danae racemosa L. Moench), a Valuable Ornamental Plant

HortScience ◽  
2013 ◽  
Vol 48 (10) ◽  
pp. 1301-1303
Author(s):  
Xiuli Shen ◽  
Guochen Yang ◽  
Zhongge (Cindy) Lu
Keyword(s):  
Morphological Variation ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Ornamental Plant ◽  
Efficient Production ◽  
Ms Medium ◽  
Culture Initiation ◽  
Shoot Number ◽  
Alternative Means

To overcome the limitations of traditional propagation, this research was initiated to develop an alternative means for efficient production of Alexandrian laurel (Danae racemosa L. Moench). An in vitro propagation protocol has been developed for Danae racemosa L. Moench using seeds as a source of material for culture initiation. Seedlings were produced after seeds were cultured for 3 month on MS (Murashige and Skoog, 1962) medium. Shoot multiplication occurred on MS medium with or without 6-benzylaminopurine (BAP) with 100% multiplication percentage. However, shoot number was significantly increased from an average of 2.8 to more than six with the addition of 5 or 25 μM BAP. Among two indole-3-butyric acid (IBA) treatments tested for rooting of seedlings, incorporation of 5 μM IBA in MS medium significantly increased rooting percentage to 86.4% compared with 71.2% without IBA. The greatest number of roots (three) was produced by 5-minute IBA pulse. However, both IBA treatments significantly reduced root length. The longest root (12.8 mm) was observed on MS medium without any IBA treatment and the shortest (6.1 mm) was produced by IBA pulse. In vitro-propagated plantlets grew well after transfer to a substrate of peat and pine bark (1:1) in the greenhouse. No morphological variation was observed.

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