scholarly journals Identification of Blood Parasite on Sacrifical Cattle Slaughtered during Idul Adha 1438 H in Surabaya City and Sidoarjo Regency

2019 ◽  
Vol 3 (2) ◽  
pp. 77
Author(s):  
Dhimar Maulud Dyahningrum ◽  
Mufasirin Mufasirin ◽  
Nenny Harijani ◽  
Poedji Hastutiek ◽  
Setiawan Koesdarto ◽  
...  
Keyword(s):  
Blood Smear ◽  
Blood Parasite ◽  
Thin Blood Smear ◽  
Blood Samples ◽  
Centrifugation Technique

The aim of this research is to detect the presence of blood parasite that infects sacrificial cattle slaughtered during idul adha 1438 H in Surabaya City and Sidoarjo Regency. This research used 147 blood samples of sacrificial cattle and used two methods, those are thin blood smear stained with Giemsa 20% and Microhematocrit Centrifugation Technique. Based on the result of the examination using a microscope with 1000x magnification, there are positive samples infected with blood parasite, that consist of Babesia sp., and Anaplasma sp.

scholarly journals Searching Before It Is Too Late: A Survey of Blood Parasites in Ctenosaura melanosterna, a Critically Endangered Reptile of Honduras

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Andrew K. Davis ◽  
Andrew C. Benz ◽  
Leslie E. Ruyle ◽  
Whitney M. Kistler ◽  
Barbara C. Shock ◽  
...  
Keyword(s):  
Blood Smear ◽  
Critically Endangered ◽  
Blood Parasites ◽  
Species At Risk ◽  
Thin Blood Smear ◽  
Blood Samples ◽  
Molecular Analyses ◽  
Critically Endangered Species ◽  
Risk Of Extinction ◽  
Host Parasite

For species at risk of extinction, any parasites they have would be expected to face a similar fate. In such cases, time is running out for efforts to identify and study their parasitic fauna before they are gone. We surveyed the hemoparasite fauna of 50 black-chested, spiny-tailed iguanas (Ctenosaura melanosterna), a critically-endangered species, on an island off the coast of Honduras. Blood samples from captured animals were tested for hemoparasites by thin blood smear and molecular analyses. Based on microscopy, two parasites were identified, a Plasmodium sp. in 14% of iguanas and a Hepatozoon sp. in 32%. For both parasites, parasitemia levels were <0.1%. Prevalence and parasitemias of Hepatozoon declined with increasing host size, a pattern differing from most prior studies of saurian reptiles. From a subset of iguanas with microscopy-confirmed Plasmodium infections, sequence analysis of 454 bp of the cytochrome b gene indicated that the Plasmodium species was distinct from known Plasmodium and was most closely related to P. chiricahuae (96.5% similarity) followed by P. mexicanum (95.8% similarity). Efforts to amplify the Hepatozoon parasite using PCR were not successful. Additional surveys and studies of this host-parasite system would be valuable, both to science and to the management of this endangered animal.

Malaria infected erythrocyte classification based on a hybrid classifier using microscopic images of thin blood smear

2016 ◽  
Vol 77 (1) ◽  
pp. 631-660 ◽  
Author(s):  
Salam Shuleenda Devi ◽  
Amarjit Roy ◽  
Joyeeta Singha ◽  
Shah Alam Sheikh ◽  
Rabul Hussain Laskar
Keyword(s):  
Blood Smear ◽  
Infected Erythrocyte ◽  
Thin Blood Smear ◽  
Hybrid Classifier ◽  
Microscopic Images

scholarly journals Diagnosis of Carrion’s Disease by Direct Blood PCR in Thin Blood Smear Negative Samples

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e92283 ◽  
Author(s):  
Juana del Valle Mendoza ◽  
Wilmer Silva Caso ◽  
Carmen Tinco Valdez ◽  
Maria J. Pons ◽  
Luis J. del Valle ◽  
...  
Keyword(s):  
Blood Smear ◽  
Thin Blood Smear ◽  
Smear Negative

scholarly journals Evaluation on the presence of Anaplasma, Ehrlichia, and Babesia spp. in goats (Capra hircus) in Cebu, the Philippines

2019 ◽  
Vol 12 (6) ◽  
pp. 774-777 ◽  
Author(s):  
Adrian P. Ybañez ◽  
Orgil V. Arrabis ◽  
Dennis Justin M. Alvarez ◽  
Eloiza May S. Galon ◽  
Rhea Mae P. Jayag ◽  
...  
Keyword(s):  
Blood Smear ◽  
Peripheral Blood Smear ◽  
The Philippines ◽  
Capra Hircus ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Blood Samples ◽  
Blood Smear Examination ◽  
Polymerase Chain ◽  
Babesia Spp

Background: Tick-borne diseases are caused by a wide variety of viruses, pathogens, and diseases. Anaplasma, Ehrlichia, and Babesia spp. are among the most known tick-borne pathogens in Asia. In the Philippines, these pathogens were already reportedly present in dogs and large ruminants, but no study has been reported yet evaluating their presence in goats. Aim: The present study aimed to evaluate the presence of Anaplasma, Ehrlichia, and Babesia spp. in goats in Cebu, the Philippines. Materials and Methods: A total of 100 blood samples from goats were collected in Cebu, the Philippines. Profile of sampled goats including age, body score, and sex was obtained. Peripheral blood smear examination and DNA extraction were performed. Nested polymerase chain reaction assay was used to evaluate the presence of Anaplasma, Ehrlichia, and Babesia spp. Results: None of the samples were found positive with Anaplasma, Ehrlichia, and Babesia spp. infection. Conclusion: Tested goats were negative with Anaplasma, Ehrlichia, and Babesia spp. and calls for continuous surveillance of these pathogens due to the reported detection of these pathogens in other livestock animals in the area.

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