scholarly journals Analysis of Genetic Diversity of 12 Genotypes of Glycine max by Using RAPD Marker

Author(s):  
Shashank A. Tidke ◽  
D. Ramakrishna ◽  
S. Kiran ◽  
Georgina Kosturkova ◽  
G.A. Ravishankar
2021 ◽  
Vol 19 (1) ◽  
pp. 20-28
Author(s):  
Abush Tesfaye Abebe ◽  
Adesike Oladoyin Kolawole ◽  
Nnanna Unachukwu ◽  
Godfree Chigeza ◽  
Hailu Tefera ◽  
...  

AbstractSoybean (Glycine max (L.) Merr.) is an important legume crop with high commercial value widely cultivated globally. Thus, the genetic characterization of the existing soybean germplasm will provide useful information for enhanced conservation, improvement and future utilization. This study aimed to assess the extent of genetic diversity of soybean elite breeding lines and varieties developed by the soybean breeding programme of the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria. The genetic diversity of 65 soybean genotypes was studied using single-nucleotide polymorphism (SNP) markers. The result revealed that 2446 alleles were detected, and the indicators for allelic richness and diversity had good differentiating power in assessing the diversity of the genotypes. The three complementary approaches used in the study grouped the germplasm into three major clusters based on genetic relatedness. The analysis of molecular variance revealed that 71% (P < 0.001) variation was due to among individual genotypes, while 11% (P < 0.001) was ascribed to differences among the three clusters, and the fixation index (FST) was 0.11 for the SNP loci, signifying moderate genetic differentiation among the genotypes. The identified private alleles indicate that the soybean germplasm contains diverse variability that is yet to be exploited. The SNP markers revealed high diversity in the studied germplasm and found to be efficient for assessing genetic diversity in the crop. These results provide valuable information that might be utilized for assessing the genetic variability of soybean and other legume crops germplasm by breeding programmes.


Crop Science ◽  
2011 ◽  
Vol 51 (3) ◽  
pp. 1080-1088 ◽  
Author(s):  
Jeong-Dong Lee ◽  
Tri D. Vuong ◽  
H. Moon ◽  
Ju-Kyung Yu ◽  
R.L. Nelson ◽  
...  

2015 ◽  
Vol 2 (1) ◽  
pp. 139
Author(s):  
Danny Laurent ◽  
Nesti F. Sianipar ◽  
Chelen _ ◽  
Listiarini _ ◽  
Ariandana Wantho

<p>Rodent tuber (Typhonium flagelliforme Lodd.) is a plant from Araceae family. The plant has high medical potential as anti-cancer agent. The information regarding Indonesian rodent tuber’s genetic diversity is not available yet. Genetic information is very important for the development of rodent tuber as medicinal plant. In this research, genetic diversity and genetic distance of three Indonesian rodent tuber’s cultivars, from Bogor, Pekalongan, and Medan, were analyzed by using RAPD molecular markers. The data obtained was analyzed by NTsys software. Out of 16 primers used in the study, the 12 primers were found to be polymorphic. There were 83 bands of DNA obtained and 31 of them were polymorphic. Dendogram analysis of the three rodent tuber cultivars showed that these cultivars were clustered into two clusters. The first cluster consists of rodent tuber Bogor and Medan. The second cluster consists of rodent tuber Pekalongan. The coefficient of similarity ranged from 0.81 to 0.87. The highest coefficient of similarity was 0.87, which was detected between rodent tuber Pekalongan and Medan. The lowest coefficient of similarity was 0.81, which was detected between rodent tuber Bogor and Pekalongan. Among these three cultivars of rodent tuber, cultivar Bogor was exclusively different.</p><p><br /><strong>Keywords</strong>: Indonesia-rodent tuber, genetic diversity, RAPD-marker</p>


2003 ◽  
Vol 180 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Gustavo Salda�a ◽  
Virginia Martinez-Alc�ntara ◽  
Jos� M. Vinardell ◽  
Ram�n Bellog�n ◽  
Jos� E. Ru�z-Sainz ◽  
...  

2012 ◽  
Vol 38 (No. 2) ◽  
pp. 69-74 ◽  
Author(s):  
M. Baránek ◽  
M. Kadlec ◽  
J. Raddová ◽  
M. Vachůn ◽  
M. Pidra

The random amplified polymorphic DNA (RAPD) technique was used to evaluate both genetic diversity among 19&nbsp;soybean accessions included in the Czech National Collection of Soybean Genotypes and their potential as a new source of genetic variations for soybean breeding programs. Only 22 of all the 40 random primers used in RAPD reactions showed polymorphism acceptable for an effective characterisation of these accessions. Altogether 122 highly reproducible RAPD fragments were generated, 55 of them were polymorphic (46%). However, because of the previously observed low degree of RAPD polymorphism in the case of Glycine max, fragments with low level of informativeness were evaluated, too. Presented results enable the selection of genetically distinct individuals. Such information may be useful to breeders willing to use genetically diverse introductions in soybean improvement process.&nbsp;


2020 ◽  
Vol 67 (6) ◽  
pp. 1587-1600
Author(s):  
Maja Žulj Mihaljević ◽  
Hrvoje Šarčević ◽  
Ana Lovrić ◽  
Zoe Andrijanić ◽  
Aleksandra Sudarić ◽  
...  

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