Comparison of Genetic Variation of Anthurium (Anthurium andraeanum) Cultivars Using SCoT, CDDP and RAPD Markers

To evaluate the genetic diversity among 10 cultivars of anthurium were performed using three molecular markers such as Start Codon Targeted (SCoT) and Conserved DNA-derived Polymorphism (CDDP), and Random Amplification of Polymorphic DNA (RAPD). Polymorphism index content (PIC) was calculated 0.39, 0.42 and 0.37 for RAPD, SCoT and CDDP, respectively. This result showed all the three molecular markers had almost an identical potential in estimating genetic diversity. Cluster analysis using SCoT, CDDP and RAPD divided the cultivars to three distinct clusters. The similarity matrix obtained through SCoT and CDDP was positively significantly correlated (r = 0.76, p < 0.01). This is the first report in which the efficiency of two targeted DNA region molecular markers (SCoT and CDDP) together with RAPD technique have been compared with each other in a set of anthurium cultivras. Results suggested that SCOT, CDDP and RAPD fingerprinting techniques are of sufficient ability to detect polymorphism in anthurium cultivars. Plant Tissue Cult. & Biotech. 28(2): 171-182, 2018 (December)

Characterization of morphological traits and RAPD polymorphism in selected forms of Kentucky bluegrass (Poa pratensis L.)

Measurements were taken of seven morphological characteristics of various forms of Poa pratensis, including ecotypes (12), cultivars (4), and breeding lines (2). Statistically significant differences relating to the average number and length of vegetative shoots and the leaf width of the forms studied were determined using Tukey’s HSD test. Evaluation of genetic polymorphism was carried out using the RAPD-PCR method. 18 primers were used for molecular analysis and 473 amplification products were obtained, in which high levels of polymorphism (84.2%) and specificity (63) were discovered. The mean value of Dice’s coefficient, the genetic similarity indicator, was about 0.7. These results indicate a significant genetic similarity between most of the forms of Poa pratensis studied. A correlation was also observed between genetic variation and phenotypic diversity of the studied forms of Poa pratensis. Convergence was found to occur between phenotype and genetic similarity among the forms. Only the dos01 468 ecotype showed discrepancies in the assessment of phenotypic and genomic variability.