Phylogenetic Analysis of 28S rRNA Gene of Indigenous  Xiphinema pachydermum

Abstract During a parasitological survey of fishes at Iguazu National Park, Argentina, specimens belonging to the allocreadiid genus Auriculostoma were collected from the intestine of Characidium heirmostigmata. The erection of the new species is based on a unique combination of morphological traits as well as on phylogenetic analysis. Auriculostoma guacurarii n. sp. resembles four congeneric species – Auriculostoma diagonale, Auriculostoma platense, Auriculostoma tica and Auriculostoma totonacapanensis – in having smooth and oblique testes, but can be distinguished by a combination of several morphological features, hosts association and geographic distribution. Morphologically, the new species can be distinguished from both A. diagonale and A. platense by the egg size (bigger in the first and smaller in the last); from A. tica by a shorter body length, the genital pore position and the extension of the caeca; and from A. totonacapanensis by the size of the oral and ventral sucker and the post-testicular space. Additionally, one specimen of Auriculostoma cf. stenopteri from the characid Charax stenopterus (Characiformes) from La Plata River, Argentina, was sampled and the partial 28S rRNA gene was sequenced. The phylogenetic analysis revealed that A. guacurarii n. sp. clustered with A. tica and these two as sister taxa to A. cf. stenopteri. The new species described herein is the tenth species in the genus and the first one parasitizing a member of the family Crenuchidae.

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Description of a New Species ofSternomoera(Crustacea: Amphipoda: Pontogeneiidae) from Japan, with an Analysis of the Phylogenetic Relationships Among the Japanese Species Based on the 28S rRNA Gene

ZOOLOGICAL SCIENCE ◽

10.2108/zs140026 ◽

2014 ◽

Vol 31 (7) ◽

pp. 475-490 ◽

Cited By ~ 14

Author(s):

Ko Tomikawa ◽

Norio Kobayashi ◽

Masaki Kyono ◽

Shin-ichi Ishimaru ◽

Mark J. Grygier

Keyword(s):

New Species ◽

Phylogenetic Relationships ◽

Rrna Gene ◽

28S Rrna ◽

Japanese Species ◽

28S Rrna Gene ◽

A New Species

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Intra-species sequence variability in 28s rRNA gene of Oesophagostomum venulosum isolated from goats of West Bengal, India

Asian Pacific Journal of Tropical Medicine ◽

10.1016/s1995-7645(10)60124-1 ◽

2010 ◽

Vol 3 (7) ◽

pp. 515-518 ◽

Cited By ~ 1

Author(s):

Subhasish Bandyopadhyay ◽

Asit Kumar Bera ◽

Sourav Sikdar ◽

Sumanta De ◽

Subhashree Ghosh ◽

...

Keyword(s):

West Bengal ◽

Rrna Gene ◽

28S Rrna ◽

Sequence Variability ◽

28S Rrna Gene

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Molecular and morphological characterisation of Xiphinema granatum n. sp. and Longidorus pisi Edward, Misra & Singh, 1964 (Dorylaimida: Longidoridae) from Iran

Nematology ◽

10.1163/156854112x638406 ◽

2012 ◽

Vol 14 (8) ◽

pp. 949-960 ◽

Cited By ~ 7

Author(s):

Majid Pedram ◽

Ebrahim Pourjam ◽

Juan E. Palomares-Rius ◽

Razieh Ghaemi ◽

Carolina Cantalapiedra-Navarrete ◽

...

Keyword(s):

New Species ◽

Species Group ◽

Rrna Gene ◽

28S Rrna ◽

Morphometric Data ◽

Molecular Analyses ◽

Morphological Characterisation ◽

28S Rrna Gene ◽

Close Relationship ◽

Female Genital

Xiphinema granatum n. sp. from pomegranate in Saveh, Markazi province, central Iran, is described and illustrated. The new species belongs to species group 8 of the genus Xiphinema sensu Loof & Luc, 1990. The new species is characterised by a body 3.5-4.1 mm long, 37-49 μm diam., odontostyle and odontophore 118-132 and 65-74 μm long, respectively, presence of sperm in the female genital tracts, uterus devoid of any Z-differentiation or spines, and abundant males with spicules 45-58 μm long. The polytomous identification codes of the new species are: A4, B4, C5a, D5, E56, F4, G23, H2, I3, J4, K2, L2. Beside morphological and morphometric data, molecular analyses of D2-D3 fragments of 28S rRNA gene placed the new species in close relationship with X. vuittenezi and X. italiae and some species belonging to Xiphinema morphospecies group 6, but clearly separated from X. index. In addition, an Iranian population of Longidorus pisi from the same locality is morphologically and molecularly characterised.

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Placement of Cacoceria and phylogenetic relationships of the xylotine genera of the tribe Milesiini (Diptera, Syrphidae: Eristalinae) based on molecular characters

Zootaxa ◽

10.11646/zootaxa.1171.1.2 ◽

2006 ◽

Vol 1171 (1) ◽

pp. 17 ◽

Cited By ~ 1

Author(s):

GUNILLA STÅHLS

Keyword(s):

Intraspecific Variation ◽

Phylogenetic Relationships ◽

Rrna Gene ◽

28S Rrna ◽

Large Fragment ◽

Mitochondrial Coi ◽

Phylogenetic Placement ◽

28S Rrna Gene ◽

Molecular Characters

The phylogenetic relationships of traditional xylotine genera of tribe Milesiini (Diptera, Syrphidae: Eristalinae) were explored using molecular character of a large fragment of the mitochondrial COI and the D2-3 region of the nuclear ribosomal 28S rRNA gene. Of particular interest was the phylogenetic placement of the enigmatic Neotropical genus Cacoceria. The ingroup included 48 taxa, and Cheilosia illustrata (Rhingiini, Eristalinae) was used as outgroup. Multiple specimens of more common taxa were sequenced for surveying levels of intraspecific variation. The combined dataset was analysed using parsimony and optimisation alignment, using the program POY. Cacoceria was resolved within the Chalcosyrphus clade. Earlier hypotheses placed the taxon in the tribe Myoleptini or Chrysogasterini, or in Xylotini without stating a closer relationship to any particular xylotine taxon. The representatives of traditional xylotine taxa were resolved as ((Hadromyia + (Brachypalpoides + Blera + Lejota) + (Xylota sg. Hovaxylota + Xylota sg. Sterphoides) + (Xylota (including Sterphus)) while Neplas and Brachypalpus were resolved in the Myoleptini albeit with very low support.

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