Phylogenetic Analysis Using the 28S rRNA Gene Reveals That the GenusParacreptotrema(Digenea: Allocreadiidae) Is Not Monophyletic; Description of Two New Genera and One New Species

Abstract During a parasitological survey of fishes at Iguazu National Park, Argentina, specimens belonging to the allocreadiid genus Auriculostoma were collected from the intestine of Characidium heirmostigmata. The erection of the new species is based on a unique combination of morphological traits as well as on phylogenetic analysis. Auriculostoma guacurarii n. sp. resembles four congeneric species – Auriculostoma diagonale, Auriculostoma platense, Auriculostoma tica and Auriculostoma totonacapanensis – in having smooth and oblique testes, but can be distinguished by a combination of several morphological features, hosts association and geographic distribution. Morphologically, the new species can be distinguished from both A. diagonale and A. platense by the egg size (bigger in the first and smaller in the last); from A. tica by a shorter body length, the genital pore position and the extension of the caeca; and from A. totonacapanensis by the size of the oral and ventral sucker and the post-testicular space. Additionally, one specimen of Auriculostoma cf. stenopteri from the characid Charax stenopterus (Characiformes) from La Plata River, Argentina, was sampled and the partial 28S rRNA gene was sequenced. The phylogenetic analysis revealed that A. guacurarii n. sp. clustered with A. tica and these two as sister taxa to A. cf. stenopteri. The new species described herein is the tenth species in the genus and the first one parasitizing a member of the family Crenuchidae.

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Description of a New Species ofSternomoera(Crustacea: Amphipoda: Pontogeneiidae) from Japan, with an Analysis of the Phylogenetic Relationships Among the Japanese Species Based on the 28S rRNA Gene

ZOOLOGICAL SCIENCE ◽

10.2108/zs140026 ◽

2014 ◽

Vol 31 (7) ◽

pp. 475-490 ◽

Cited By ~ 14

Author(s):

Ko Tomikawa ◽

Norio Kobayashi ◽

Masaki Kyono ◽

Shin-ichi Ishimaru ◽

Mark J. Grygier

Keyword(s):

New Species ◽

Phylogenetic Relationships ◽

Rrna Gene ◽

28S Rrna ◽

Japanese Species ◽

28S Rrna Gene ◽

A New Species

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Phylogenetic Analysis of 28S rRNA Gene of Indigenous Xiphinema pachydermum

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2019.807.233 ◽

2019 ◽

Vol 8 (07) ◽

pp. 1960-1968

Author(s):

Anju Sharma ◽

Satish K. Sharma ◽

Kiran Rana ◽

Anil Kumar Verma

Keyword(s):

Phylogenetic Analysis ◽

Rrna Gene ◽

28S Rrna ◽

28S Rrna Gene

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Molecular and morphological characterisation of Xiphinema granatum n. sp. and Longidorus pisi Edward, Misra & Singh, 1964 (Dorylaimida: Longidoridae) from Iran

Nematology ◽

10.1163/156854112x638406 ◽

2012 ◽

Vol 14 (8) ◽

pp. 949-960 ◽

Cited By ~ 7

Author(s):

Majid Pedram ◽

Ebrahim Pourjam ◽

Juan E. Palomares-Rius ◽

Razieh Ghaemi ◽

Carolina Cantalapiedra-Navarrete ◽

...

Keyword(s):

New Species ◽

Species Group ◽

Rrna Gene ◽

28S Rrna ◽

Morphometric Data ◽

Molecular Analyses ◽

Morphological Characterisation ◽

28S Rrna Gene ◽

Close Relationship ◽

Female Genital

Xiphinema granatum n. sp. from pomegranate in Saveh, Markazi province, central Iran, is described and illustrated. The new species belongs to species group 8 of the genus Xiphinema sensu Loof & Luc, 1990. The new species is characterised by a body 3.5-4.1 mm long, 37-49 μm diam., odontostyle and odontophore 118-132 and 65-74 μm long, respectively, presence of sperm in the female genital tracts, uterus devoid of any Z-differentiation or spines, and abundant males with spicules 45-58 μm long. The polytomous identification codes of the new species are: A4, B4, C5a, D5, E56, F4, G23, H2, I3, J4, K2, L2. Beside morphological and morphometric data, molecular analyses of D2-D3 fragments of 28S rRNA gene placed the new species in close relationship with X. vuittenezi and X. italiae and some species belonging to Xiphinema morphospecies group 6, but clearly separated from X. index. In addition, an Iranian population of Longidorus pisi from the same locality is morphologically and molecularly characterised.

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Revision of the South American genus Gaujonia Dognin (Noctuidae, Pantheinae) with descriptions of five new genera and twenty-one new species

ZooKeys ◽

10.3897/zookeys.985.51622 ◽

2020 ◽

Vol 985 ◽

pp. 71-126

Author(s):

Jose I. Martinez

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Morphological Characters ◽

The Other ◽

South American ◽

New Combinations ◽

New Genera ◽

The South ◽

Molecular Analyses ◽

One New Species

The endemic Neotropical genus Gaujonia Dognin is revised. Morphological characters and a phylogenetic analysis demonstrate paraphyletic relationships among the species. Four different groups are interpreted to represent four different genera. The G. arbosi group is the only remaining clade in the genus Gaujonia, and the other groups have been arranged into three new genera: Milleranagen. nov., Oculicattusgen. nov., and Cicadoformagen. nov. Additionally, two other genera Cicadomorphusgen. nov., and Gaujopteragen. nov. were found using morphological and molecular analyses based on some specimens that were misidentified as Gaujonia spp. A total of five new genera, three new combinations (Cicadoforma vau-nigrum Hampson, comb. nov., Oculicattus renifera Hampson, comb. nov., and Millerana arbosioides Dognin, comb. nov.) and 21 new species (Cicadoforma ocelotussp. nov., Cicadomorphus chicharrasp. nov., Cicadomorphus chuyasp. nov., Cicadomorphus falkasiskasp. nov., Cicadomorphus lilianaesp. nov., Gaujonia bichusp. nov., Gaujonia chiqyaqsp. nov., Gaujonia kanakusikasp. nov., Gaujonia sourakovisp. nov., Gaujoptera amsasp. nov., Millerana austinisp. nov., Millerana cajassp. nov., Millerana cundinamarquensissp. nov., Millerana matthewsaesp. nov., Millerana tigrinasp. nov., Oculicattus bolivianasp. nov., Oculicattus brehmisp. nov., Oculicattus incasp. nov., Oculicattus raizaesp. nov., Oculicattus schmidtisp. nov., and Oculicattus uturunkusp. nov.) are established.

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Morphological and molecular characterisation of Tylencholaimellus zeinabadensis sp. n. (Dorylaimida: Tylencholaimellidae) from Iran, with additional data on five known species of the genus

Nematology ◽

10.1163/15685411-00003238 ◽

2019 ◽

Vol 21 (6) ◽

pp. 597-612

Author(s):

Nasir Vazifeh ◽

Gholamreza Niknam ◽

Habibeh Jabbari ◽

Arezoo Naghavi ◽

Reyes Peña-Santiago

Keyword(s):

New Species ◽

Additional Data ◽

Rrna Gene ◽

28S Rrna ◽

Molecular Characterisation ◽

Gene Sequences ◽

Pharyngeal Bulb ◽

Molecular Analyses ◽

28S Rrna Gene ◽

Region 10

Summary Six species of the genus Tylencholaimellus, one new and five known, collected in virgin and cultivated soils of East Azarbaijan province, Iran, are studied. Tylencholaimellus zeinabadensis sp. n. is morphologically characterised by its 1.2-1.7 mm long body, lip region 10-13 μm wide and offset by deep and broad constriction, odontostyle 16-20 μm long, stylet 22-30 μm long, neck 186-234 μm long, pharyngeal bulb 30-43 μm long, anterior uterine sac 55-103 μm long or 0.9-1.7 corresponding body diam. long, V = 34-41, tail short and rounded to conoid (20-30 μm, c = 45-83, c′ = 0.5-1.0), spicules 39-50 μm long, and one or two ventromedian supplements with hiatus. Molecular analyses using the D2-D3 expansion regions of the 28S rRNA gene sequences of the new species confirmed the monophyly of this genus based upon currently available data. A population of T. affinis is described and illustrated, and new data (morphometrics, pictures and distribution) are provided for T. loofi, T. paracinctus, T. polonicus and T. striatus.

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Review of the New World genera of the Leafhopper Tribe Erythroneurini (Hemiptera: Cicadellidae: Typhlocycbinae)

Illinois Natural History Survey Bulletin ◽

10.21900/j.inhs.v37.121 ◽

2006 ◽

Vol 37 (1-6) ◽

pp. 118-190 ◽

Cited By ~ 7

Author(s):

Christopher H. Dietrich ◽

Dmitry A. Dmitriev

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Type Species ◽

New World ◽

New Genus ◽

Morphological Characters ◽

New Genera ◽

Previously Treated ◽

One New Species

The genus-level classification of New World Erythroneurini is revised based on results of a phylogenetic analysis of 100 morphological characters. The 704 known species are placed into 18 genera. Erasmoneura Young and Eratoneura Young, previously treated as subgenera of Erythroneura Fitch, and Erythridula Young, most recently treated as a subgenus of Arboridia Zachvatkin, are elevated to generic status. Three species previously included in Erasmoneura are placed in a new genus, Rossmoneura (type species, Erythroneura tecta McAtee). The concept of Erythroneura is thereby narrowed to include only those species previously included in the nominotypical subgenus. New World species previously included in Zygina Fieber are not closely related to the European type species of that genus and are therefore placed in new genera. Neozygina, n. gen., based on type species Erythroneura ceonothana Beamer, includes all species previously included in the “ceonothana group”, and Zyginama, n. gen., based on type species Erythroneura ritana Beamer, includes most species previously included in the “ritana group” of New World Zygina. Five additional new genera are described to include other previously described North American Erythroneurini: Hepzygina, n. gen., based on type species Erythroneura milleri Beamer and also including E. aprica McAtee; Mexigina, n. gen., based on type species Erythroneura oculata McAtee; Nelionidia, n. gen., based on type species N. pueblensis, n. sp., three additional new species, and Erythroneura amicis Ross; Neoimbecilla, n. gen., based on type species Erythroneura kiperi Beamer and one new species; and Illinigina, n. gen., based on type species Erythroneura illinoiensis Gillette. Five new genera, based on previously undescribed species, are also recognized: Aztegina, n. gen, based on A. punctinota, n. sp., from Mexico; Amazygina, n. gen., based on type species A. decaspina, n. sp., and three additional new species from Ecuador; Hamagina, n. gen., based on type species H. spinigera, n. sp., and two additional new species from Peru and Ecuador; Napogina, n. gen., based on type species N. recta, n. sp., and one additional new species from Ecuador; Perugina, n. gen., based on type species P. denticula, n. sp., from Peru; and Spinigina, n. gen., based on type species S. hirsuta, n. sp., and an additional new species from Peru. Phylogenetic analysis suggests that the New World Erythroneurini consist of three lineages resulting from separate invasions from the Old World.

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Descriptions of Trichodorus lownsberyi sp. n., Nanidorus minor (Colbran, 1956) Siddiqi, 1974 (Nematoda: Trichodoridae) and Longidorus quercus sp. n. (Nematoda: Longidoridae) from Mexico

Nematology ◽

10.1163/15685411-bja10092 ◽

2021 ◽

pp. 1-15

Author(s):

Ignacio Cid del Prado Vera ◽

Sergei A. Subbotin ◽

Wilfrida Decraemer

Keyword(s):

New Species ◽

Phylogenetic Relationships ◽

Phylogenetic Trees ◽

The Body ◽

Rrna Gene ◽

28S Rrna ◽

Gene Sequences ◽

28S Rrna Gene ◽

Buxus Sempervirens ◽

Stubby Root Nematode

Summary A new species of stubby root nematode, Trichodorus lownsberyi sp. n., collected from soil around Buxus sempervirens at Montecillo Campus, Colegio de Postgraduados, Mexico, is described and illustrated. Trichodorus lownsberyi sp. n. is characterised by the abundant males with a characteristic spicule shape, the narrower part about mid-blade having visible bristles in most specimens, and the presence and position of the three ventromedian cervical papillae, which are all anterior to the secretory-excretory pore. Females possess a rhomboid-shaped vagina and oval to rounded triangular vaginal sclerotised pieces with a pore-like vulva. The phylogenetic relationships of T. lownsberyi sp. n. with related species were constructed using the ITS2 rRNA and the D2-D3 expansion segments of 28S rRNA gene sequences. Trichodorus lownsberyi sp. n. was a sister species to T. viruliferus in the phylogenetic trees. A Mexican population of Nanidorus minor from a peach orchard is also described and illustrated. The new needle nematode, Longidorus quercus sp. n., was recovered from soil around roots of oak, Quercus crassipes, from Cerro Jusda ‘El Diablo’ in Mexico State. Longidorus quercus sp. n. females are characterised by the C-shaped posterior end of the body after fixation, L = 4.9 (3.9-5.6) mm, a = 71 (60.8-93.2), lip region rounded, sometimes slightly flattened, marked by depression, odontostylet 170 (144-206) μm long, hemispherical to bluntly conoid tail and no males. The new species was characterised using the D2-D3 of 28S rRNA and COI gene sequences. The phylogenetic relationships of L. quercus sp. n. with other Longidorus species were reconstructed using the D2-D3 of 28S rRNA gene sequences.

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