scholarly journals In-vitro Efficacy of Bio-agents against Sudden Death Syndrome (wilt) Disease of Soybean Caused by Fusarium oxysporum f. sp. virguliforme

2021 ◽  
Vol 10 (2) ◽  
pp. 2808-2812
Author(s):  
S. S. Gote P. H. Ghante ◽  
U. A. Asalkar V. S. Mete ◽  
S. K. Deshmukh
Keyword(s):  
Sudden Death ◽  
Fusarium Oxysporum ◽  
Wilt Disease ◽  
Sudden Death Syndrome

scholarly journals Tomato Endophytic Bacteria Composition and Mechanism of Suppressiveness of Wilt Disease (Fusarium oxysporum)

2021 ◽  
Vol 12 ◽  
Author(s):  
Zeyu Zhang ◽  
Ji Li ◽  
Zengqiang Zhang ◽  
Youzhou Liu ◽  
Yuquan Wei
Keyword(s):  
Fusarium Oxysporum ◽  
Endophytic Bacteria ◽  
High Throughput Sequencing ◽  
High Efficiency ◽  
Abiotic Factors ◽  
Wilt Disease ◽  
Agricultural Practice ◽  
Pathogen Invasion ◽  
Disease Diagnostics

Tomato wilt disease, caused by the Fusarium oxysporum is an ever-increasing threat for agricultural production, and unreasonable fertilization and pesticide abuse caused environmental challenge. Increasing evidence suggested that microbiomes or those associated with crops, played key roles on plant health. Plant disease dynamics were affected by multiple biotic and abiotic factors including phytopathogen population density, the genetic type of the pathogen and the host, in particular, the composition and assembly of the host-associated microbiome. However, it was unclear how pathogen invasion interaction and correlate with endophytic bacterial communities in natural field conditions. To study this, we sampled temporally the tomato plants that were exposed to F. oxysporum invasions over one crop season. High-throughput sequencing were performed to explore the correlation between agricultural practice, pathogen invasion, and endophytic microbiota communities. Results showed that pathogen invasion had clear effect on the endophytic and a strong link between increased pathogen densities and reduced abundance of Bacillus sp., which are crucial taxonomy for suppressiveness to F. oxysporum in vitro and in greenhouse condition. In summary, monitoring the dynamics of endophytic bacteria communities and densities of pathogen could thus open new avenue for more accurate disease diagnostics and high-efficiency screening antagonisms methods in the future, and our results will broaden the agricultural view of beneficial microbiota as biological control agents against plant pathogen.

scholarly journals Transcriptome analysis of mycorrhizal and non-mycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome

2018 ◽  
Author(s):  
N. Marquez ◽  
M. L. Giachero ◽  
A. Gallou ◽  
H. J. Debat ◽  
S. Declerck ◽  
...  
Keyword(s):  
Sudden Death ◽  
Mycorrhizal Fungi ◽  
Severe Disease ◽  
Arbuscular Mycorrhizal ◽  
Sudden Death Syndrome ◽  
Transcriptional Analysis ◽  
Experimental Conditions ◽  
Cell Wall Modification ◽  
Transcriptional Changes

ABSTRACTSoilborne pathogens represent a threat to agriculture causing important yield losses. The “Sudden Death Syndrome” (SDS), a severe disease in soybean is caused by a complex of Fusarium species. This pathosystem has been widely investigated and several strategies were proposed to manage SDS. Although a decrease in symptoms and in the level of root tissue infection particularly by F. virguliforme was observed in presence of arbuscular mycorrhizal fungi (AMF), biological control based on AMF has received less attention. Here we report the results, under strict in vitro culture experimental conditions, a transcriptional analysis in mycorrhizal versus non-mycorrhizal soybean plantlets upon infection by F. virguliforme. An important transcriptional reprogramming was detected following infection by the pathogen. Results revealed 1768 and 967 differentially expressed genes in the AMF-colonized (+AMF+Fv) and non-colonized (−AMF+Fv) plants, respectively. Major transcriptional changes, corresponded to defence response related genes belonging to secondary metabolism, stress and signalling categories. The +AMF+Fv treatment showed the largest number of upregulated genes related to defence, as those encoding for disease resistance proteins, WRKY transcription factors, auxins, receptors kinases, and proteases. Only few genes had primed expression in +AMF+Fv treatment, as those coding for a thaumatin-like protein (TLP) and a pleiotropic drug resistance (PDR) protein. Moreover, +AMF+Fv showed a significant number of downregulated genes related to cell wall modification and peroxidases than – AMF+Fv treatment. This detailed insight increases our knowledge on the transcriptional changes and the potential metabolic pathways involved in the enhanced resistance/tolerance of mycorrhizal plants upon infection with F. virguliforme.

Pengembangan Teknologi Pengendalian Penyakit Layu Tomat (Fusarium oxysporum f. sp. lycopersici) yang Ramah Lingkungan dengan Menggunakan Mikroorganisme Saprofit

Agrotek ◽  
2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Derek Kornelis Erari ◽  
Eko Agus Martanto
Keyword(s):  
Biological Control ◽  
Fusarium Oxysporum ◽  
Growth And Development ◽  
Wilt Disease ◽  
Common Disease ◽  
Microbial Antagonism ◽  
The Earth ◽  
Tomato Wilt

Fusarium wilt disease is a common disease for tomatoes plant which damages plant although it is intensively cultivated. Biological control by microbial antagonism is environmentally friendly compare to the use of fungicide. This research was aimed to study the capability of Bacillus subtilis, Pseudomonas fluorescens, Gliocladium fimbriatum and Trichoderma viridae that are locally specific in controll Fusarium oxysporum f.sp. lycopersici� cause tomato wilt disease. Evaluation of F.o. f.sp. lycopersici� antagonism capability was done by in vitro and in vivo.� In vitro antagonism treatment used the ouble plating treatment on Potato Dextose Agar.� In vivo antagonism treatment was done by gave antagonism agent and F.o. f.sp. lycopersici� together to the earth medium. The result of the study revealed that (1) B. subtilis, G. fimbriatum and T. viridae can pressure the growth and development �F.o. f.sp. lycopersici which cause tomato wilt disease both in vitro and in vivo, (2) In vitro the average of the prevented pathogen growth was as follow: B. subtilis (55.52%), G. fimbriatum (36.77%) and� T. viridae (35.87%), (3) In vivo treatment by the earth medium was not significantly different, and (4) In vivo the ability of prevent disease intensity was as follow : B. subtilis (62.35%), G. fimbriatum (49.95%) and� T. viridae (49.95%).

scholarly journals Evaluation of Different Fungicides and Bioagents for the Management of Chickpea Wilt (Fusarium oxysporum f. sp. ciceri)

2020 ◽  
pp. 19-30
Author(s):  
Seethiya Mahajan ◽  
Deepak Kumar ◽  
Santosh Kumar Singh ◽  
Deepak Mahajan ◽  
Devendra Kumar ◽  
...  
Keyword(s):  
Grain Yield ◽  
Fusarium Oxysporum ◽  
Disease Incidence ◽  
Maximum Growth ◽  
Wilt Disease ◽  
Copper Oxychloride ◽  
Maximum Increase ◽  
Chickpea Wilt ◽  
Highly Sensitive

The incidence of the chickpea wilt disease caused by Fusarium oxysporum f. sp. ciceri ranged between 8.11 - 21.67 and 10.98 - 23.99 per cent with an overall mean disease incidence of 15.64 and 16.86 per cent respectively during Rabi seasons of 2016-17 and 2017-18 in surveys conducted fortnightly in the different chickpea growing areas of Jammu sub-tropics. The maximum growth inhibition of pathogen i.e., 78.44 per cent was observed by local isolate T. harzianum (Th-III) and P. fluorescens (Pf-III) was least effective in controlling the growth of pathogen i.e., 53.00 per cent in In vitro studies. Among chemicals, carbendazim at 100 ppm was significantly effective in inhibiting the growth of pathogen (98.67%), while copper oxychloride and mancozeb showed inhibition of 83.11 and 82.22 per cent, respectively. Both the antagonists were highly sensitive to propiconazole (Tilt), carbendazim (Bavistin), difenoconazole (Score), iprodione + carbendazim (Quintal) and metalaxyl (Ridomil), giving no growth of T. viride (TV-III) and T. harzianum (TH-III). TMTD (Thiram) recorded least inhibition of both the bioagents. Under field conditions, bioagents recorded maximum seed germination of 90.21 and 90.07 per cent, whereas least germination was recorded in mancozeb (84.17 and 83.10%). Carbendazim recorded lowest disease incidence (14.92 and 14.97%) over untreated control (44.42 and 45.77%). However, maximum grain yield was recorded in azoxystrobin + T. harzianum-III (14.30 and 14.57 q/ha) and azoxystrobin + T. viride-III (14.15 and 14.38 q/ha) and the least grain yield was recorded in mancozeb (10.58 and 10.64 q/ha) during Rabi 2016-17 and 2017-18. Maximum increase in grain yield was recorded in azoxystrobin + T. harzianum-III (62.31 and 62.43%) followed by azoxystrobin + T. viride-III (62.61 and 60.87%) during Rabi 2016-17 and 2017-18.

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