In Vitro Assessment of Fungicide and Biocontrol Agent against Alternaria Leaf Spot of Cauliflower (Brassica oleraceaea var. botrytis)

2018 ◽  
Vol 5 (9) ◽  
pp. 35-43
Author(s):  
Kartik Uniyal ◽  
Narendra Kumar ◽  
Zahoor Ahmad Shah
Keyword(s):  
Leaf Spot ◽  
Biocontrol Agent ◽  
Alternaria Leaf Spot ◽  
In Vitro Assessment

scholarly journals Alternaria leaf spot of broccoli caused by Alternaria alternata in Bangladesh

2021 ◽  
Author(s):  
Sayma T. Nira ◽  
Md. Farhad Hossain ◽  
Nur Uddin Mahmud ◽  
Oliul Hassan ◽  
Md. Tofazzal Islam ◽  
...  
Keyword(s):  
Alternaria Alternata ◽  
Leaf Spot ◽  
Maximum Growth ◽  
Pathogenicity Test ◽  
Culture Studies ◽  
Alternaria Leaf Spot ◽  
Cultural Methods ◽  
Molecular Phylogenetic ◽  
Alternaria Sp

  This study aimed to isolate and characterise the pathogen associated with Alternaria leaf spot on broccoli and to evaluate the inhibitory effects of fungicides against it. We isolated and identified the fungal pathogen as Alternaria sp. using morphological and cultural methods. Based on the aligned sequences of the internal transcribed spacer (ITS) and molecular phylogenetic analysis by the neighbour-joining method, the isolates (Ab1 and Ab2) were confirmed as Alternaria alternata. The conidia of the isolates were dark brown, cylindrical, obclavate to muriform. The conidiophores were olivaceous brown, septate, and branched. The conidial morphology of the isolates ranged from 52.4–92.4 × 10–20 μm with 2–6 transverse and 0–3 longitudinal septa. Both isolates yielded positive results in the pathogenicity test on broccoli leaves by developing brown and circular spots with concentric rings on the leaves surrounded by yellow halos. The culture studies revealed that the maximum growth of the pathogen was obtained at 30 °C and pH 6.0. Tilt 250 WC showed the highest potential in suppressing the mycelial growth of the A. alternata in vitro at a concentration as low as 50 µg/mL. The results from this study contributed to the positive identification of the pathogen and characterised A. alternata as a destructive pathogen of broccoli which may be successfully controlled by the fungicide Tilt.  

scholarly journals In vitro evaluation of fungicides against Alternaria leaf spot of cotton

2020 ◽  
Vol 8 (4) ◽  
pp. 3571-3575
Author(s):  
Kaki Pranaya ◽  
Bharati N Bhat ◽  
G Uma Devi ◽  
S Triveni
Keyword(s):  
Leaf Spot ◽  
In Vitro Evaluation ◽  
Alternaria Leaf Spot

The influence of cysteine, cysteine analogs, and other amino acids on spore germination of Alternaria species

1991 ◽  
Vol 69 (11) ◽  
pp. 2353-2356 ◽  
Author(s):  
Donald J. Daigle ◽  
Peter J. Cotty
Keyword(s):  
Amino Acids ◽  
Spore Germination ◽  
Leaf Spot ◽  
Acid Group ◽  
Carboxylic Acid Group ◽  
Potato Dextrose Broth ◽  
Alternaria Leaf Spot ◽  
Cotton Plants ◽  
Alternaria Species

The influence of amino acids on spore germination of Alternaria eassiae was investigated to develop a basis for improvement of mycoherbicide formulations. At a 1% concentration, the majority of amino acids exhibited little or no effect on spore germination in vitro. However, aspartic and glutamic acids stimulated germination, and serine, tryptophan, cysteine, and phenylalanine inhibited spore germination. Cysteine was the strongest inhibitor of germination and was active in the presence of potato dextrose broth, which is normally stimulatory. One percent cysteine reduced germination 96% after 4 h. Derivitization of the mercaptan or amino group but not the carboxylic acid group diminished the ability to inhibit spore germination. Cysteine also inhibited spore germination of Alternaria crassa and Alternaria macrospora. Application of cysteine reduced development of Alternaria leaf spot on cotton plants inoculated with A. macrospora. The results demonstrate diverse effects of amino acids on spore behavior and suggest cysteine or similar compounds may be useful in preventing diseases caused by Alternaria species. Key words: amino acids, biological control, cysteine, germination, potato dextrose broth, Alternaria leaf spot.

scholarly journals OCCURRENCE OF ALTERNARIA LEAF SPOT DISEASE ON ALOE VERA AND ITS MANAGEMENT

2019 ◽  
Vol 31 (1) ◽  
pp. 75-80
Author(s):  
Muhammad M Rasheed ◽  
Amer Habib ◽  
Mustansar Aslam ◽  
Zeeshan Mansha ◽  
Abdul Rehman ◽  
...  
Keyword(s):  
Disease Control ◽  
Plant Extracts ◽  
Leaf Spot ◽  
Mycelial Growth ◽  
Disease Incidence ◽  
Aloe Vera ◽  
Leaf Spot Disease ◽  
Dry Rot ◽  
Alternaria Leaf Spot

Aloe vera is a valuable medicinal crop of the world. It suffers from several diseases but among them alternaria leaf spot and dry rot is most serious fungal disease which effects the commercial production and quality losses to Aloe vera. A survey was conducted to estimate the prevalence of dry rot disease in different nurseries located in urban areas of Faisalabad. During this survey maximum disease incidence was recorded in Horti club nursery (100%) and minimum disease incidence recorded at Faiz baho nursery and Qadir baksh form (13.8%). Among in-vitro tested fungicides Score gives maximum mycelial growth inhibition (89.5%) and maximum disease control (17.7%) as compared to control and other tested chemicals. Among in-vitro tested plant extracts Neem gives maximum mycelial growth inhabitation (41.2%) and in green house gives 14.2% disease control. Out of four tested fungicides and plant extracts Score and Neem gives best results against A. alternata mycelial growth and disease control.

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