scholarly journals The Inhibitory Effects and Positive Contributions of Live Foods on Protease Activities of Meagre, Argyrosomus regius (Asso 1801) Larvae in vitro Assay

2018 ◽  
Author(s):  
Gürkan Diken ◽  
Orhan Demir ◽  
Mehmet Naz
Keyword(s):  
Marine Fish ◽  
Fish Larvae ◽  
Survival Ratio ◽  
Inhibitory Effects ◽  
Growth And Survival ◽  
In Vitro Techniques ◽  
Artemia Nauplii ◽  
Marine Fish Larvae ◽  
Argyrosomus Regius

The aim of this study was to gather preliminary data about the potential inhibitory effects and contributions of live foods used from 3 to 32 days after hatching (DAH) in routine feeding protocols on protease activities of meagre, Argyrosomus regius (Asso 1801) larvae, using in vitro techniques. Enriched rotifer, Artemia nauplii and Artemia metanauplii were tested in the present study. The highest values of protease activities of meagre larvae at 7 DAH in 2013 and 2014 years were established. The lowest values at 15 DAH in 2013 and at 20 DAH in 2014 were observed. Protease activities of enriched rotifer, Artemia nauplii, and Artemia metanauplii were 21.76±0.31, 36.00±1.48–29.33±0.93, and 416.44±19.7–403.53±11.85 U/mg protein, respectively (p< 0.05). The highest inhibitions of live foods were observed at 7 DAH. The positive contributions of live food Artemia metanauplii’s on protease activities of meagre larvae were significant (p< 0.05). The inhibitory effects and positive contributions of live foods on survival and growth rates of meagre larvae, should be taken into account in meagre and other marine fish larvae’s future studies. Cysteine protease activities of Artemia sp. should be investigated to provide the higher growth and survival ratio from the feeding protocols used in marine fish larvae.

scholarly journals The Inhibitory Effects and Positive Contributions of Live Foods on Protease Activities of Meagre, Argyrosomus regius (Asso 1801) Larvae in vitro Assay

2018 ◽  
Author(s):  
Gürkan Diken ◽  
Orhan Demir ◽  
Mehmet Naz
Keyword(s):  
Marine Fish ◽  
Fish Larvae ◽  
Survival Ratio ◽  
Inhibitory Effects ◽  
Growth And Survival ◽  
In Vitro Techniques ◽  
Artemia Nauplii ◽  
Marine Fish Larvae ◽  
Argyrosomus Regius

The aim of this study was to gather preliminary data about the potential inhibitory effects and contributions of live foods used from 3 to 32 days after hatching (DAH) in routine feeding protocols on protease activities of meagre, Argyrosomus regius (Asso 1801) larvae, using in vitro techniques. Enriched rotifer, Artemia nauplii and Artemia metanauplii were tested in the present study. The highest values of protease activities of meagre larvae at 7 DAH in 2013 and 2014 years were established. The lowest values at 15 DAH in 2013 and at 20 DAH in 2014 were observed. Protease activities of enriched rotifer, Artemia nauplii, and Artemia metanauplii were 21.76±0.31, 36.00±1.48–29.33±0.93, and 416.44±19.7–403.53±11.85 U/mg protein, respectively (p< 0.05). The highest inhibitions of live foods were observed at 7 DAH. The positive contributions of live food Artemia metanauplii’s on protease activities of meagre larvae were significant (p< 0.05). The inhibitory effects and positive contributions of live foods on survival and growth rates of meagre larvae, should be taken into account in meagre and other marine fish larvae’s future studies. Cysteine protease activities of Artemia sp. should be investigated to provide the higher growth and survival ratio from the feeding protocols used in marine fish larvae.

Size-dependent foraging of larval Atlantic cod (Gadus morhua)

2004 ◽  
Vol 82 (8) ◽  
pp. 1380-1389 ◽  
Author(s):  
Velmurugu Puvanendran ◽  
Karine Salies ◽  
Benjamin Laurel ◽  
Joseph A Brown
Keyword(s):  
Marine Fish ◽  
Gadus Morhua ◽  
Atlantic Cod ◽  
Prey Selection ◽  
Fish Larvae ◽  
Growth And Survival ◽  
Size Dependent ◽  
Marine Fish Larvae ◽  
Key Factor ◽  
Foraging Environment

Most marine fish larvae are thought to be gape-limited predators, and the presence of suitably sized prey at the appropriate time in the foraging environment is a key factor for their growth and survival. Two experiments were carried out: in experiment 1, we investigated feeding of Atlantic cod (Gadus morhua L., 1758) larvae from 5 to 35 days post hatch on prey of three different sizes: two different-sized strains of rotifers, small rotifers (SR) and large rotifers (LR), and Artemia Leach, 1819 nauplii (AN), or an equal mixture of the three types of prey (MIX). In experiment 2, cod larvae were fed SR, LR, or a combination of SR and LR (MIX-R) at concentrations of 1500 and 4000 prey·L–1 from 4 to 20 days post hatch. Feeding incidence, number of prey in the gut, mouth gape, and prey selection were measured. In experiment 1, feeding incidence was initially higher in the SR treatment, while larvae failed to start feeding in either the LR or the AN treatment at 5 dph. Larvae in the LR treatment started feeding at 8 dph, and feeding incidence was comparable to that in the SR treatment, but the total number of prey eaten was higher in the SR than in the LR treatment until 20 dph. Larvae did not start feeding on AN until 26 dph, although they attacked them from 5 dph. In the MIX-R treatment in experiment 2, larvae fed selectively on LR at 4000 prey·L–1, whereas no selection was observed at 1500 prey·L–1. Our results suggest that cod larvae are gape-limited predators and the concentration of prey affects prey selection.

scholarly journals The Inhibitory Effects and Positive Contributions of Live Foods on Protease Activities of Meagre, Argyrosomus regius (Asso 1801), Larvae in vitro Assay

Fishes ◽  
2019 ◽  
Vol 4 (1) ◽  
pp. 5
Author(s):  
Gürkan Diken ◽  
Orhan Demir ◽  
Mehmet Naz
Keyword(s):  
Live Food ◽  
Larval Feeding ◽  
Inhibitory Effects ◽  
Vitro Assay ◽  
In Vitro Techniques ◽  
Exogenous Enzyme ◽  
Live Feeds ◽  
Argyrosomus Regius

The determination of exogenous enzyme contributions in live food is important in larval feeding. This study investigated the potential inhibitory effects and contributions of live foods used from 3 to 32 days after hatching (DAH) on protease activities of meagre (Argyrosomus regius) larvae (ML), using in vitro techniques. Enriched rotifer (R), Artemia nauplii (A0), and Artemia metanauplii (A1) were tested. The highest values of protease activities of ML were determined to occur at 7 DAH for the years 2013 and 2014. The lowest values were observed at 15 DAH in 2013 and at 20 DAH in 2014. Protease activities of R, A0, and A1 were 21.76 ± 0.31, 36.00 ± 1.48–29.33 ± 0.93, and 416.44 ± 19.7–403.53 ± 11.85 U/mg protein, respectively (p < 0.05). The highest inhibitions of live foods were observed at 7 DAH. This situation was related to the inadequacy of protease contributions of live feeds , despite the highest protease value of larvae at 7 DAH (p < 0.05). The positive contributions of A1 live food on the protease activities of ML were significant (p < 0.05). The inhibitory effects and positive contributions of live foods on the survival and growth rates of ML should be taken into account for future studies considering the larvae of ML and other marine fish.

Isolation, morphometry, and culture of Colurella sp. (Rotifera: Ploimida)

2013 ◽  
Vol 1 (2) ◽  
pp. 143
Author(s):  
Petrus P Letsoin ◽  
Henneke Pangkey ◽  
Julius Sampekalo ◽  
Inneke F.M Rumengan ◽  
Stenly Wullur ◽  
...  
Keyword(s):  
Body Size ◽  
Marine Fish ◽  
Fish Larvae ◽  
Nannochloropsis Oculata ◽  
Tropical Fish ◽  
Sampling Location ◽  
Larval Rearing ◽  
Brachionus Rotundiformis ◽  
Marine Fish Larvae ◽  
Significant Difference

The rotifer Brachionus rotundiformis (total body length 240.59±10.24 μm, lorica length 175.28±9.18 μm, and lorica width 124.28±7.76μm) is commonly used as starter food in the larval rearing of marine fish. But, larvae of some marine tropical fish species required starter food with body size smaller than B. rotundiformis. The present study was aimed to isolate minute rotifers from nature and to assess the possibility of culturing these rotifers. Sampling of rotifers was conducted in an estuary of Mangket (Kema-Minut), using plankton net (mesh size 40 µm). A trial of culturing the rotifers was conducted at salinities of 10, 20 and 30 ppt by using a microalga, Nannochloropsis oculata. A species of rotifer identified as Colurella sp. (family Lepadellidae) was successfully isolated from the sampling location. Body size of Colurella sp. was extremely small (Total length 123.22±5.45 μm, lorica length 95.96±3.81 μm, and lorica width 53.57±3.11 μm), which were smaller than Brachionus rotundiformis SS-type as a conventional starter food for marine fish larvae.  Results of culturing the minute rotifer Colurella sp. showed that the species grew well at salinities of 10, 20 and 30 ppt with no significant difference among treatments (ANOVA, p>0.05), indicating a potential use of minute rotifer Colurellasp. as starter food for marine fish larvae. Rotifera Branchionus rotundiformis (ukuran tubuh: panjang total 240,59±10,24 μm, panjang lorika 175,28±9,18 μm, dan lebar lorika 124,28±7,76μm) sering digunakan sebagai pakan awal pemeliharaan larva ikan laut. Namun, larva beberapa spesis ikan laut tropis membutuhkan pakan awal berukuran tubuh lebih kecil dari Branchionus rotundiformis. Penelitian ini bertujuan untuk mendapatkan minute rotifer dari alam (berukuran tubuh lebih kecil dari B. rotundiformis) dan menguji kemungkinan pemeliharaannya. Sampling rotifer dilakukan di perairan estuari Desa Mangket (Kema-Minut), menggunakan plankton net (ukuran mata jaring 40 µm). Uji coba pemeliharaan dilakukan pada salinitas (10, 20, dan 30 ppt) dengan menggunakan Nannochloropsis oculata. Satu spesies minute rotifer yang teridentifikasi sebagai Colurella sp. (family Lepadellidae) berhasil diisolasi dari lokasi sampling. Colurella sp. memiliki ukuran tubuh sangat kecil (panjang total [PT] 123,22±5,45 µm, panjang lorika [PL] 95,96±3,81 µm, dan lebar lorik [LL] 53,57±3,11 µm) yang mana lebih kecil dari Branchionus rotundiformis tipe-SS sebagai pakan awal larva ikan laut. Hasil uji coba pemeliharaan minute rotifer Colurella sp. menunjukkan bahwa spesis ini dapat tumbuh pada salinitas 10, 20, dan 30 ppt dengan perbedaan kepadatan populasi yang tidak signifikan antar perlakuan (Uji ANOVA, p > 0.05) mengindikasikan potensi pemanfaatan minute rotifer Colurella sp. sebagai pakan awal larva ikan laut.

scholarly journals A Preliminary Survey on the Planktonic Biota in a Hypersaline Pond of Messolonghi Saltworks (W. Greece)

Diversity ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 270
Author(s):  
George N. Hotos
Keyword(s):  
New Species ◽  
Marine Fish ◽  
Broad Spectrum ◽  
Fish Larvae ◽  
Live Food ◽  
Preliminary Survey ◽  
Genus Level ◽  
Marine Fish Larvae ◽  
Alternaria Sp ◽  
Different Strains

During a survey in 2015, an impressive assemblage of organisms was found in a hypersaline pond of the Messolonghi saltworks. The salinity ranged between 50 and 180 ppt, and the organisms that were found fell into the categories of Cyanobacteria (17 species), Chlorophytes (4 species), Diatoms (23 species), Dinoflagellates (1 species), Protozoa (40 species), Rotifers (8 species), Copepods (1 species), Artemia sp., one nematode and Alternaria sp. (Fungi). Fabrea salina was the most prominent protist among all samples and salinities. This ciliate has the potential to be a live food candidate for marine fish larvae. Asteromonas gracilis proved to be a sturdy microalga, performing well in a broad spectrum of culture salinities. Most of the specimens were identified to the genus level only. Based on their morphology, as there are no relevant records in Greece, there is a possibility for some to be either new species or strikingly different strains of certain species recorded elsewhere.

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