PELATIHAN PENERAPAN SISTEM “APARTEMENT” BAGI BUDIDAYA CACING SUTRA PADA KELOMPOK PEMBUDIDAYA IKAN CUPANG DI DESA KAMPUNG BARU, KECAMATAN LANGSA LAMA, KOTA LANGSA

ABSTRAKCacing sutra merupakan pakan hidup yang disukai ikan khususnya larva ikan. Hal ini karena ukurannya yang kecil sehingga mudah dimakan. Kesesuaian pakan hidup yang diberikan akan semakin membuat besar larva ikan hidup dan tumbuh. Tujuan dari kegiatan pengabdian kepada masyarakat ini yaitu membantu mitra untuk meningkatkan hasil produksi ikan cupang dengan pemberian cacing sutra yang dibudidayakan dengan sistem apartement. Metode yang digunakan melalui beberapa tahap, yaitu sosialisasi, pendampingan, monitoring dan evaluasi. Sosialisasi dilakukan untuk memberikan informasi yang lebih jelas tentang maksud dan tujuan tim mengadakan pelatihan penerapan sistem Apartement. Pendampingan dilakukan berupa pembuatan sistem apartemen dan pembuatan pakan cacing sutra dengan menggunakan ampas tahu yang difermentasikan menggunakan larutan EM 4 dan molase selama 3 hari, kemudian pakan siap digunakan. Hasil monitoring dan evaluasi mitra mampu serta terampil membuat wadah sistem apartemen serta pakan cacing sutra dari ampas tahu. Selanjutnya mitra juga sudah sangat faham bagaimana pola kerja dalam melakukan budidaya cacing serta dengan menggunakan sistem apartemen. Kata kunci: cacing sutra; sistem apartement; ikan cupang; desa kampung baru. ABSTRACTTubifex sp. are live food that is preferred by fish, especially fish larvae. This is because of its small size so it is easy to eat. The suitability of the live feed given will make the larger fish larvae live and grow. The purpose of this community service activity is to help partners to increase the production of betta fish by giving silk worms that are cultivated with the apartment system. The method used goes through several stages, namely socialization, mentoring, monitoring and evaluation. The socialization was carried out to provide clearer information about the goals and objectives of the team holding training on the application of the Apartment system. Assistance is provided in the form of building an apartment system and making feed for Tubifex sp. by using tofu dregs which was fermented using EM 4 solution and molasses for 3 days, then the feed was ready for use. The results of monitoring and evaluation of partners are capable and skilled in making apartment system containers and tubifex sp. feed. from tofu dregs. Furthermore, partners also understand very well how the work pattern in cultivating Tubifex sp. using the apartment system. Keywords: tubifex sp. apartment system; Betta splend; kampung baru village.

Features of Growing Live Food Artemia salina in Aquaculture

The problem of providing Russians with safe high quality aquaculture products remains essential. It is necessary to achieve the objective of developing the production of live food to cultivate baby fish in Russia, as well as in the Irkutsk region. The aim of the article is to study and introduce the technology of obtaining Artemia salina nauplii. An analysis of contemporary literature on biology, as far as the issues of aquaculture are concerned, proves the necessity of selecting a technology of cultivating nauplii to feed fish at certain age stages. The article summarizes the data on the habitat of Artemia salina in the Russian Federation. The data on the larval stage of the object of the research are specified. Scientific foundations of technological aspects with regard to hatching of hydrobionts Artemia salina larvae are presented. Environmental optimum conditions for swim-up of Artemia depending on the degree of water salinity, oxygen saturation and luminance during the incubation period of small crustaceans are specified. The reasons for giving live food to baby fish are substantiated.

Microalgal Diet Influences the Nutritive Quality and Reproductive Investment of the Cyclopoid Copepod Paracyclopina nana

Copepods represent an interesting alternative or a complement live food to brine shrimps and rotifers commonly used in aquaculture. They constitute the natural prey of many fish species and therefore do not require a potential nutritional enrichment. But an optimization of the microalgal diets used to feed copepods is essential to improve their mass culture. This study examined the effects of seven microalgal diets, namely single-species diets of Rhodomonas salina (R), Tisochrysis lutea (T), and Pavlova lutheri (=Diacronema lutheri) (P), two-species diets (R + T, T + P, and R + P), and a three-species diet (R + T + P), on the fatty acid and monosaccharide composition of the cyclopoid copepod Paracyclopina nana as well as its reproductive investment. Experiments were run during 15 days in 10-L beakers; starting with nauplii collected from a large 300-L batch culture. Copepods fatty acid contents were studied, particularly the relative amounts of docosahexaenoic acid (DHA) and eicosa-pentaenoic acid (EPA). The R + T, R, and T diets induced the highest total fatty acid amount in copepods. R + T and R also generated the lowest DHA/EPA ratios in copepods due to high EPA contents. The highest value of total monosaccharides was found in copepods fed with R + T + P. Diets R + T and R induced the greatest prosome volumes and clutch volumes in ovigerous females. Both prosome volume and clutch volume in P. nana ovigerous females were correlated to the individual EPA amount. The results demonstrated that all diets including R. salina enhanced the productivity of P. nana in mass culture, particularly when combined with T. lutea. R. salina, and T. lutea induced complementary fatty acid and monosaccharide profiles, confirming that R + T represents the best microalgae combination for productive culture of P. nana. Conversely, P. lutheri did not enhance the nutritional profile nor the fecundity of P. nana in the culture. This study is the first to demonstrate that R. salina is a suitable microalga for productive mass culture of P. nana for use as live food in aquaculture.

A Preliminary Survey on the Planktonic Biota in a Hypersaline Pond of Messolonghi Saltworks (W. Greece)

During a survey in 2015, an impressive assemblage of organisms was found in a hypersaline pond of the Messolonghi saltworks. The salinity ranged between 50 and 180 ppt, and the organisms that were found fell into the categories of Cyanobacteria (17 species), Chlorophytes (4 species), Diatoms (23 species), Dinoflagellates (1 species), Protozoa (40 species), Rotifers (8 species), Copepods (1 species), Artemia sp., one nematode and Alternaria sp. (Fungi). Fabrea salina was the most prominent protist among all samples and salinities. This ciliate has the potential to be a live food candidate for marine fish larvae. Asteromonas gracilis proved to be a sturdy microalga, performing well in a broad spectrum of culture salinities. Most of the specimens were identified to the genus level only. Based on their morphology, as there are no relevant records in Greece, there is a possibility for some to be either new species or strikingly different strains of certain species recorded elsewhere.

Non-Specific Immunity Associated Gut Microbiome in Aristichthys nobilis under Different Rearing Strategies

To understand the intestinal microbial diversity and community structure of bighead carp (Aristichthys nobilis) under different feeding strategies, 39 fish from three groups (A: 9 fish, natural live food only; B: 15 fish, natural live food + fish formulated feeds; C: 15 fish, natural live food + fish formulated feed + lactic acid bacteria) were obtained for the high throughput 16S rRNA gene sequencing. We first examined five non-specific immunity indications of the carp—lysozyme (LZM), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GSH-PX), and superoxide dismutase (SOD). Interestingly, the composition of gut microbiota and related non-specific immune indices were affected by the feeding treatment of the bighead carp. Notably, all enzyme activity indexes were significantly different (p < 0.01) in the spleen and three enzyme activity indexes (LZM, GSH-PX, and SOD) had significant differences in the hepatopancreas (p < 0.001) of the carp from the three groups. The 16S rRNA gene sequencing showed higher diversity in groups B and C. Compared to group A, the relative abundance of Actinobacteria increased significantly and the relative abundance of Proteobacteria and Firmicutes decreased significantly in groups B and C at the phylum level. Functional analysis revealed the association between non-specific immune indicators and import genera in the hepatopancreas and spleen of bighead carp. This study provides new insights into the gut microbiomes and non-specific immune of bighead carp.

A Preliminary Survey on the Planktonic Biota in a Hypersaline Pond of Messolonghi Saltworks (W. Greece)

During a survey in 2015 an impressive assemblage of organisms were found in a hypersaline pond of the Messolonghi saltworks. The salinity ranged between 50 and 180 ppt and the organisms recorded fell in the categories of Cyanobacteria (17 species), Chlorophytes (4 species), Diatoms (23 species), Dinoflagellates (1 species), Protozoa (40 species), Rotifers (8 species), Copepods (1 species), Artemia sp., one nematode and Alternaria sp. (Fungi). Fabrea salina was the most prominent protist in all samples and salinities. This ciliate has the potential to be a live-food candidate for marine fish larvae. Asteromonas gracilis proved a sturdy microalga performing excellently in a broad spectrum of culture salinities ies. Most of the specimens were identified only to the genus level and, based on their morphology, as there are no relevant records in Greece, there is a possibility for some of them to be either new species or strikingly different strains of certain species recorded elsewhere.