Hollow Gold Nanoshell Modified Electrode as Electrochemical Biosensor for Hemoglobin and Its Analytical Application

A new electrochemical biosensor based on the immobilization of horseradish peroxidase(HRP) on TiO2 nanoneedles modified electrodes has been fabricated. The direct electrochemical response of HRP immobilized on the modified electrode was dramatically enhanced. The immobilized HRP displayed a couple of stable and well-defined redox peaks with a formal potential of -0.379 V (vs. SCE). The HRP/TiO2 nanoneedles modified electrode exhibited a remarkable electrocatalytic activity toward the oxidation of H2O2. The amperometric response to H2O2 showed a linear range of 4–700μmol/L, with the calculated detection limit of 0.78 μmol/L at a signal-to-noise ratio of three. The modified electrode displayed an acceptable reproducibility and good stability. The new HRP/TiO2 nanoneedles matrix is expected to have wide applications for enzymes and proteins immobilization and direct electron transfer study and opened a way for low conductivity electrode biosensors.

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Interaction of prednisone with dsDNA at silver nanoparticles/poly(glyoxal-bis(2-hydroxyanil))/dsDNA modified electrode and its analytical application

Bioelectrochemistry ◽

10.1016/j.bioelechem.2018.11.003 ◽

2019 ◽

Vol 126 ◽

pp. 56-63 ◽

Cited By ~ 5

Author(s):

Gözde Aydoğdu Tığ ◽

Derya Koyuncu Zeybek ◽

Bülent Zeybek ◽

Şule Pekyardımcı

Keyword(s):

Silver Nanoparticles ◽

Modified Electrode ◽

Analytical Application

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Gold nanoparticles modified electrode via simple electrografting of in situ generated mercaptophenyl diazonium cations for development of DNA electrochemical biosensor

Biosensors and Bioelectronics ◽

10.1016/j.bios.2010.07.076 ◽

2011 ◽

Vol 26 (5) ◽

pp. 1947-1952 ◽

Cited By ~ 27

Author(s):

Feng Li ◽

Yan Feng ◽

Pingjun Dong ◽

Limin Yang ◽

Bo Tang

Keyword(s):

Gold Nanoparticles ◽

Modified Electrode ◽

Electrochemical Biosensor ◽

Dna Electrochemical Biosensor

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Optimization Design of Electrochemical Biosensors Based on Gold Nanomaterials

Nanoscience and Nanotechnology Letters ◽

10.1166/nnl.2020.3219 ◽

2020 ◽

Vol 12 (9) ◽

pp. 1079-1086

Author(s):

Haitao Hao

Keyword(s):

Modified Electrode ◽

Optimization Design ◽

Electrochemical Biosensor ◽

Design Method ◽

High Sensitivity ◽

Dna Biosensor ◽

Gold Nanorod ◽

Simple Preparation ◽

Optimal Design Method ◽

Gold Nanomaterials

At present, chemical biosensors still have poor reproducibility and stability due to that the density of nanomaterials particles is not ideal. Therefore, an optimal design method of electrochemical biosensor based on gold nanomaterials is proposed. Two types of sensors, namely DNA biosensor and immune biosensor, were designed in this work. A DNA biosensor with gold nanorod nanocomposite modified electrode was prepared, and its electrical activity was measured by Methylene blue. In contrast, an electrochemical immune sensor with gold nanorod-modified electrode was prepared and used for detection of alpha fetoprotein. The experimental results show that the designed DNA biosensor and electrochemical immune sensor have the advantages of simple preparation, high sensitivity, good selectivity and good stability.

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