Effect of Intermittent Administration of Teriparatide (Parathyroid Hormone 1-34) on Bone Morphogenetic Protein-Induced Bone Formation in a Rat Model of Spinal Fusion

2014 ◽  
Vol 96 (13) ◽  
pp. e107-1-8 ◽  
Author(s):  
Tokimitsu Morimoto ◽  
Takashi Kaito ◽  
Masafumi Kashii ◽  
Yohei Matsuo ◽  
Tsuyoshi Sugiura ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Bone Formation ◽  
Spinal Fusion ◽  
Bone Morphogenetic Protein ◽  
Rat Model ◽  
Intermittent Administration ◽  
Morphogenetic Protein

10:4666. Intermittent Administration of Human Parathyroid Hormone (1-34) Accelerates the Maturity of Spinal Fusion Mass in Rat Model

2006 ◽  
Vol 6 (5) ◽  
pp. 33S-34S
Author(s):  
Yuichiro Abe ◽  
Masahiko Takahata ◽  
Manabu Ito ◽  
Kuniyoshi Abumi ◽  
Akio Minami
Keyword(s):  
Parathyroid Hormone ◽  
Spinal Fusion ◽  
Rat Model ◽  
Human Parathyroid Hormone ◽  
Intermittent Administration

scholarly journals Enhanced Bone Morphogenetic Protein-2-Induced Ectopic and Orthotopic Bone Formation by Intermittent Parathyroid Hormone (1–34) Administration

2010 ◽  
Vol 16 (12) ◽  
pp. 3769-3777 ◽  
Author(s):  
Diederik H.R. Kempen ◽  
Lichun Lu ◽  
Theresa E. Hefferan ◽  
Laura B. Creemers ◽  
Andras Heijink ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Bone Formation ◽  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein

Bone morphogenetic protein gene therapy for the induction of spinal arthrodesis

1998 ◽  
Vol 4 (2) ◽  
pp. E14 ◽  
Author(s):  
Tord D. Alden ◽  
Gerald R. Hankins ◽  
Elisa J. Beres ◽  
David F. Kallmes ◽  
Gregory A. Helm
Keyword(s):  
Gene Therapy ◽  
Bone Formation ◽  
Spinal Fusion ◽  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 2 ◽  
Lumbosacral Spine ◽  
Ct Reconstruction ◽  
Morphogenetic Protein ◽  
Adenoviral Construct

Gene therapy has many potential applications in neurosurgery. One application involves bone morphogenetic protein-2 (BMP-2), a low-molecular-weight glycoprotein that induces bone formation in vivo. Numerous studies have demonstrated that the BMP-2 protein can enhance spinal fusion. This study was undertaken to determine whether direct injection of an adenoviral construct containing the BMP-2 gene can be used for spinal fusion. Twelve athymic nude rats were used in this study. Recombinant, replication-defective type-5 adenovirus with a universal promoter and BMP-2 gene (Ad-BMP-2) was used. A second adenovirus constructed with a universal promoter and ß-galactosidase (ß-gal) gene (Ad-ß-gal) was used as a control. Seven and one-half microliters of virus was injected percutaneously and paraspinally at the lumbosacral junction in three groups (four animals each): 1) Ad-BMP-2 bilaterally, 2) Ad-BMP-2 on the right, Ad-ß-gal on the left, and 3) Ad-ß-gal bilaterally. Computerized tomography (CT) scans of the lumbosacral spine were obtained at 3, 5, and 12 weeks. At 12 weeks, the animals were killed for histological inspection. Ectopic bone formation was seen both on three-dimensional CT reconstruction and histologically in all rats at sites treated with Ad-BMP-2. Histological analysis revealed bone at different stages of maturity adjacent to the spinous processes, laminae, and transverse processes. This study clearly demonstrated that it is possible to produce in vivo endochondral bone formation by using direct adenoviral construct injection into the paraspinal musculature, which suggests that gene therapy may be useful for spinal fusion in the future.

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