The Mode of Action on the Vitelline Envelope of Xenopus Hatching Enzyme as Studied by Its Two Molecular Forms

Summary5,-5'-diphenyl-2-thiohydantoin (DPTH) is an effective inhibitor of thyroxine (T4) stimulation of α-glycerophosphate dehydrogenase in rat liver mitochondria. Because this finding indicated a possible tool for future study of the mode of action of thyroxine, the ultrastructural and biochemical effects of DPTH and/or thyroxine on rat liver mere investigated.Rats were fed either standard or DPTH (0.06%) diet for 30 days before T4 (250 ug/kg/day) was injected. Injection of T4 occurred daily for 10 days prior to sacrifice. After removal of the liver and kidneys, part of the tissue was frozen at -50°C for later biocheailcal analyses, while the rest was prefixed in buffered 3.5X glutaraldehyde (390 mOs) and post-fixed in buffered 1Z OsO4 (376 mOs). Tissues were embedded in Araldlte 502 and the sections examined in a Zeiss EM 9S.Hepatocytes from hyperthyroid rats (Fig. 2) demonstrated enlarged and more numerous mitochondria than those of controls (Fig. 1). Glycogen was almost totally absent from the cytoplasm of the T4-treated rats.

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Mode of action of calcimycin (A 23187) — II — A study of its interactions ith alkali and alkali-earth cations in methanol-water mixtures

Journal de Chimie Physique ◽

10.1051/jcp/1985820899 ◽

1985 ◽

Vol 82 ◽

pp. 899-906 ◽

Cited By ~ 5

Author(s):

C. Tissier ◽

J. Juillard ◽

D.W. Boyd ◽

A.M. Albrecht-Gary

Keyword(s):

Mode Of Action ◽

Alkali Earth ◽

A 23187

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Antibiotic mode of action profiling (Biomap) as a platform for the discovery of novel natural product antibiotics

Planta Medica ◽

10.1055/s-0032-1320307 ◽

2012 ◽

Vol 78 (11) ◽

Author(s):

W Ruh Wong ◽

AG Oliver ◽

RG Linington

Keyword(s):

Natural Product ◽

Mode Of Action

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Molecular Forms of Human Protein C: Comparison and Distribution in Human Adult Plasma

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651025 ◽

1989 ◽

Vol 62 (03) ◽

pp. 902-905 ◽

Cited By ~ 8

Author(s):

Brian S Greffe ◽

Marilyn J Manco-Johnson ◽

Richard A Marlar

Keyword(s):

Heavy Chain ◽

Protein C ◽

Molecular Forms ◽

Post Translational Modification ◽

Single Chain ◽

Beta Form ◽

Sds Page ◽

Dependent Protein ◽

Adult Plasma ◽

The Difference

SummaryProtein C (PC) is a vitamin K-dependent protein which functions as both an anticoagulant and profibrinolytic. It is synthesized as a single chain protein (SC-PC) and post-transla-tionally modified into a two chain form (2C-PC). Two chain PC consists of a light chain (LC) and a heavy chain (HC). The present study was undertaken to determine the composition of the molecular forms of PC in plasma. PC was immunoprecipitated, subjected to SDS-PAGE and Western blotting. The blots were scanned by densitometry to determine the distribution of the various forms. The percentage of SC-PC and 2C-PC was found to be 10% and 90% respectively. This is in agreement with previous work. SC-PC and the heavy chain of 2C-PC consisted of three molecular forms (“alpha”, “beta”, and “gamma”). The “alpha” form of HC is the standard 2C form with a MW of 40 Kd. The “beta” form of HC has also been described and has MW which is 4 Kd less than the “alpha” form. The “gamma” species of the SC and 2C-PC has not been previously described. However, its 3 Kd difference from the “beta” form could be due to modification of the “beta” species or to a separate modification of the alpha-HC. The LC of PC was shown to exist in two forms (termed form 1 and form 2). The difference between these two forms is unknown. The molecular forms of PC are most likely due to a post-translational modification (either loss of a carbohydrate or a peptide) rather than from plasma derived degradation.

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